Method for purifying long-chain diacid
A technology of long-chain dibasic acid and purification method, which is applied in the field of purifying long-chain dibasic acid, can solve the problems of hindering the production of long-chain dibasic acid, difficult to handle sodium sulfate solution, etc. The effect of acid-base depletion
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Embodiment 1-5 and comparative example 1
[0060] The preparation method of secondary seed liquid in following embodiment 1-5 and comparative example 1:
[0061] Prepare secondary seed liquid according to the method of patent ZL200410018255.7 embodiment 1:
[0062] 1) Take the glycerol tube strain of Candida tropicalis (CCTCC NO: M203052) and inoculate it into 500 mL seeds filled with 200 mL liquid medium (10 g / kg peptone, 5 g / kg yeast extract, 10 g / kg glucose, natural pH) Cultivate in the bottle at 35°C, 300rpm shaker for 10-15 hours;
[0063] 2) Take the above-mentioned shaking flask seeds, insert them into a 10L fermenter equipped with 5L seed medium (10g / kg peptone, 10g / kg yeast extract, 10g / kg glucose, natural pH), and cultivate them at 35°C for 24 hours to produce Get the first-grade seed liquid for subsequent use;
[0064] 3) With 16M 3 Medium 20M 3 In the fermenter, the above-mentioned first-level seed liquid is inserted, and the second-level seed tank cultivation is started. The fermentation medium formul...
Embodiment 1
[0073] installed in 100M 3 200M of medium 3 In the fermenter, the secondary seed liquid added to the seed liquid starts to ferment. The composition of the fermentation medium is: glucose 30g / L, KH 2 PO 4 5g / L, yeast extract 20g / L, corn steep liquor 15g / L, urea 2.5g / L, NaCl2.0g / L, KNO 3 7g / L, prepared with pure water, natural pH, sterilized at 121℃. Dissociation of C12 alkanes and fed sugars. Cultivate at 29°C under the conditions of ventilation rate 0.5vvm and tank pressure 1.0Mpa. 20 hours before fermentation, the pH was natural, and the growth of bacteria was the main factor. When the growth optical density (OD 620 ) greater than 0.6 (diluted 30 times), start to add C12 alkanes 8% in batch mode, and then add an alkane once every 8 hours to control the concentration of alkane in the fermentation broth to maintain about 5% (V: V, relative to the initial volume of fermentation), At the same time, adjust the pH to 6.5, 48-120 hours until the tank is released, and adjust ...
Embodiment 2
[0080] The method for preparing the long-chain dibasic acid is the same as that of Example 1, the difference is that: in the above process, the pH value is adjusted to 7.2 with ammonia water every 4 hours until 48-120 hours into the tank, and the fermented dodecane dibasic acid is obtained. Liquid 1000L, wherein dibasic acid content 153g / L.
[0081] Add water to the above fermentation broth to adjust the mass concentration of the dibasic acid to 10.5%, adjust the pH to 8.5 with ammonia gas, heat to 60° C., and filter through a ceramic membrane with a pore size of 0.1 micron. The area of the ceramic membrane used is 0.84 square meters, the pressure in front of the membrane is set at 0.4MPa, and the membrane liquid is received.
[0082] The received membrane serum was decolorized by adding 5wt% powdered activated carbon (relative to the amount of long-chain dibasic acid contained in the solution) at 60° C., and filtered to obtain a clear liquid. The received clarified liquid ...
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