A polymer film potential type anti-biofouling sensor based on graphene oxide antifouling layer and its application

A polymer film and biofouling technology, which is applied to instruments, scientific instruments, and material analysis through electromagnetic means, can solve problems such as shortened service life of electrodes, electrode biofouling, and decreased detection performance of electrodes, so as to reduce damage , simple operation, avoid the effect of chemical modification process

Active Publication Date: 2022-04-05
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Application Information

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Problems solved by technology

However, in practical applications, the electrode surface of this type of electrode is prone to biofouling, which greatly affects the thermodynamic response process of the electrode, resulting in longer response time, lower detection performance of the electrode, and shorter service life of the electrode.
In order to reduce the damage caused by biofouling in environmental water bodies, researchers have developed a variety of antifouling technologies, such as: ultrasonic method, radiation method, applied current method, etc., but most of the technologies are aimed at marine artificial equipment (such as: ships, buoys, etc.), unable to expand to the field of small detection equipment-sensors
In addition, most of the current antifouling research on polymer membrane ion-selective electrodes focuses on how to improve the anti-protein adhesion of ion-selective electrodes in biological samples, and has not yet involved how to improve the anti-biological properties of electrodes in environmental water Adhesion
Improving the resistance of electrodes to biofouling in the environment without affecting the detection performance of polymer membrane potential sensors is still a big problem
In addition, the functional modification of ion-selective electrodes is often used to immobilize biomolecules and construct biosensors, but there is little research on the surface modification of ion-selective electrodes based on inorganic nanomaterials.

Method used

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  • A polymer film potential type anti-biofouling sensor based on graphene oxide antifouling layer and its application
  • A polymer film potential type anti-biofouling sensor based on graphene oxide antifouling layer and its application
  • A polymer film potential type anti-biofouling sensor based on graphene oxide antifouling layer and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Taking the detection of calcium ions as an example, the specific preparation steps of the polymer membrane potential type anti-biofouling sensor based on the graphene oxide antifouling layer are as follows:

[0033] a. The configuration of the calcium ion selective membrane solution: a total of 360mg of the mixture of PVC particles, o-nitrophenyloctyl ether, calcium ionophore and tetrakis (3,5-two (trifluoromethyl) phenyl) sodium borate, wherein For 31.8wt% PVC particles, 63.7wt% o-nitrophenyloctyl ether, 2.3wt% calcium ionophore and 2.2wt% tetrakis (3,5-bis(trifluoromethyl)phenyl) sodium borate, moved to 3.6 mL tetrahydrofuran solution, stirred for 6h to disperse evenly, and the electrode sensitive membrane solution was obtained.

[0034] b. The pretreatment process of the disk electrode: taking the glassy carbon disk electrode as an example, after the polished disk electrode is ultrasonically cleaned with absolute ethanol and deionized water, the surface of the electr...

Embodiment 2

[0037] Taking the detection of calcium ions as an example, using the above embodiments to obtain the potential detection of the sensor for calcium ions:

[0038] A. the antifouling calcium ion selective electrode prepared as described in Example 1 is used as a working electrode, and the Ag / AgCl electrode is used as a reference electrode to detect the potential of different concentrations of calcium ions;

[0039] b. place the antifouling calcium ion selective electrode obtained above in 10 -3 M CaCl 2 Activation in solution overnight. Use CaCl 2 1M calcium ion stock solution (containing 0.5M NaCl) configured with NaCl, add calcium ion stock solution to the 0.5M NaCl bottom solution, so that the calcium ion in the bottom solution is 10 -4 、10 -3.5 、10 -3 、10 -2.5 、10 -2 、10 -1.5 、10 -1 M, and then use the above-mentioned detection system to detect, and record the potential values ​​produced by different concentrations of calcium ions (see figure 2 ). After correctin...

Embodiment 3

[0041] Taking the detection of calcium ions as an example, the above-mentioned embodiment is used to obtain the potential response of the sensor to calcium ions after being soaked in high-concentration bacterial solution for several days:

[0042] a. Cultivation and preparation of high-concentration bacterial solution: mix 1mL seawater and 9mL LB medium evenly and place in a shaker at 37°C for overnight culture, then take 1mL overnight culture medium containing bacteria and mix evenly with 9mL LB medium. Incubate at 37°C for 2 hours in a shaker. The bacteria were separated from the medium by centrifugation, and the bacteria were washed 3 times with sterile saline, and then the bacteria were dispersed in sterile saline to obtain a concentration of 10 8 or 10 9 CFU mL -1 The bacterial suspension was used for later use.

[0043] b. the polymer film potential type antifouling sensor obtained in embodiment 1 is placed in above-mentioned step a) bacterial suspension (10 9 CFU mL...

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Abstract

The invention relates to a potential type anti-biofouling sensor, in particular to a polymer film potential type anti-biofouling sensor based on a graphene oxide antifouling layer and an application thereof. The polymer film potential type anti-biofouling sensor is composed of a conventional disc electrode, a polymer sensitive film and a graphene oxide layer; one end of the conventional disc electrode is adhered to the polymer sensitive film and the graphene oxide layer in sequence. The polymer membrane potential type anti-biofouling sensor based on the graphene oxide antifouling layer of the present invention can improve the hydrophilicity and killing properties of the polymer sensitive membrane by means of the graphene oxide material, so as to prevent the formation of biological adhesion and biofilm Function, realize the anti-biofouling performance of the polymer membrane potential sensor, so as to solve the biofouling problem that the polymer membrane potential sensor often faces when it is applied in complex water bodies. The polymer membrane potential type anti-biofouling sensor based on the graphene oxide antifouling layer of the present invention has the advantages of simple preparation and long duration of antifouling performance.

Description

technical field [0001] The invention relates to a potential type anti-biofouling sensor, in particular to a polymer film potential type anti-biofouling sensor based on a graphene oxide antifouling layer and an application thereof. Background technique [0002] Among the various environmental detection methods that have been developed so far, the electrochemical method has been widely used in environmental, medical and food safety detection due to its advantages of simple operation, fast detection speed, and easy miniaturization. Polymer sensitive membrane electrode is a relatively common electrochemical sensor, which is not affected by the color, turbidity and viscosity of the sample, and has the advantages of continuous and rapid detection of the analyte. It is widely used in the actual sample site and in situ analysis. wide. However, in practical applications, the electrode surface of this type of electrode is prone to biofouling, which greatly affects the thermodynamic r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/333
CPCG01N27/3335
Inventor 江天甲秦伟戚龙斌
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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