A Strain of Stenotrophomonas and Its Application on Codonopsis Codonopsis

A technology of Stenotrophomonas and Codonopsis pilosula, applied in the field of microorganisms, can solve the problems of discounted effect, long half-life, damage, etc., and achieve the effects of improving quality safety, good ecological safety, and solving pollution problems

Active Publication Date: 2020-08-28
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bifenthrin is a broad-spectrum insecticide with a long half-life in soil, which not only pollutes agricultural ecosystems and water bodies, but also poses potential hazards to the quality and safety of Codonopsis pilosula and human health
At present, most of the domestic and foreign studies focus on the degrading bacteria of a single pesticide, but there are few studies on the degrading bacteria that degrade different types of pesticides simultaneously.
[0005] At the same time, the additional application of microorganisms other than the original soil environment will affect and damage the original soil micro-ecological environment, and the colonization of foreign strains is poor, requiring long-term application, and a large amount of application can ensure the effectiveness of microbial agents, resulting in the application of The cost of microbial agents increases, but the effect is discounted, and the ecological safety still needs to be evaluated

Method used

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  • A Strain of Stenotrophomonas and Its Application on Codonopsis Codonopsis
  • A Strain of Stenotrophomonas and Its Application on Codonopsis Codonopsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation of Stenotrophomonas ZBD-a-03-I CGMCC No.17102

[0033] Take 5g of soil sample (collected from Zhang County, Gansu Province) into a triangular flask, add 50ml of sterile water, seal it and shake it on a shaker for 20 minutes to make a suspension, absorb 5ml and add it to 100ml of nitrogen-fixing bacteria enrichment medium, 28 Cultivate at ℃, and after 72 hours, absorb 5ml of the bacterial solution and add 100ml of nitrogen-fixing bacteria enrichment medium to continue the cultivation, repeat the cultivation three times, and then isolate a single colony. Separation adopts the plate streaking method and gradient dilution method: the plate streaking method is to use the inoculation loop to dip the bacterial liquid on the solid medium of Ashubei for streaking and separation; the gradient dilution method is to divide the cultured bacterial liquid at 10 -2 、10 -4 、10 -6 、10 -8 Dilution After making a bacterial suspension with sterile water, draw 0.1ml an...

Embodiment 2

[0034] Example 2 Identification of Stenotrophomonas ZBD-a-03-I CGMCC No.17102.

[0035] The isolated and purified ZBD-a-03-I strain was identified from the following three aspects.

[0036] 1. Morphological identification

[0037]Cultivate the ZBD-a-03-I isolated in Example 1 and observe the colony, mainly including the size, color, transparency, humidity, and surface state of the colony (whether it is flat, convex, wrinkled, depressed, etc.) , Colony edge state (whether neat, irregular, radial, etc.). Microscopic examination was performed after staining with biological stains.

[0038] The results showed that the colony of strain ZBD-a-03-I obtained after separation and purification: the diameter of the colony was 6-7 mm, the color of the colony was light yellow, the colony was opaque, the surface of the colony was moist and slightly raised, and the edges of the colony were neat. Microscopic examination results were rod-shaped, Gram-negative, with flagella and no spores. ...

Embodiment 3

[0047] Example 3 Nitrogenase activity test of Stenotrophomonas ZBD-a-03-I CGMCC No.17102

[0048] The Stenotrophomonas ZBD-a-03-I CGMCC No.17102 obtained by the separation and purification of Example 1 was tested for nitrogenase activity, and the acetylene reduction method was used. The specific method is as follows: ZBD-a-03-I was prepared and obtained Strain suspension (cells, 1 x 10 8 ml -1 ), take 1ml and inoculate it in a serum bottle filled with 5ml of semi-solid Ashubei culture medium, and incubate at 28°C for 48h. Replace the cap of the serum bottle with a rubber stopper, draw out 10% of the gas with a sterile syringe, then inject 10% acetylene, and incubate at 28°C for 36h. Use a sterile syringe to extract 0.2ml of the mixed gas from the serum bottle and inject it into the gas chromatograph, measure the amount of ethylene produced, and calculate the nitrogenase activity according to the following formula. N=hxCV / (24.9hst). where N is the nitrogenase activity, that...

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Abstract

The invention provides a strain of Stenotrophomonas ( Stenotrophomonas sp. ) ZBD‑a‑03‑I, the strain is now preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the registration number of the preservation center is: CGMCC No.17102. ZBD‑a‑03‑I has high nitrogenase activity, can degrade carbendazim and bifenthrin, and has strong tolerance to Codonopsis radix extract, ZBD‑a‑03‑I bacterial solution or The microbial agent with ZBD‑a‑03‑I as the core strain can be used as the exclusive nitrogen-fixing bacterial fertilizer of Codonopsis codonopsis, and it can also be used to repair the soil with residual carbendazim and bifenthrin.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a strain of Stenotrophomonas and its application on Radix Codonopsis. Background technique [0002] Codonopsis Codonopsis is a traditional tonic medicine commonly used in China. It has the effects of invigorating the middle and Qi, strengthening the spleen and lungs. Modern research shows that Codonopsis pilosula contains various sugars, phenols, sterols, volatile oils, baicalein glucoside, saponins and trace alkaloids, which have the functions of enhancing immunity, dilating blood vessels, lowering blood pressure, improving microcirculation, and enhancing hematopoietic function. In addition, it can improve the leukopenia caused by chemotherapy and radiotherapy. Gansu is one of the main producing areas of Codonopsis ginseng. The annual output of Baitiao Codonopsis ginseng in Weiyuan accounts for more than 60% of the total output of Codonopsis ginseng in the country, and Weiyuan is known ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/80B09C1/10C12R1/01
CPCB09C1/10B09C2101/00C05G3/00C12N1/205C12R2001/01
Inventor 杜文静丁品刘锦霞李晶李娜武建荣张建军
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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