Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

NPC culture medium, preparation method for exosome and application

A culture medium and exosome technology, applied in biochemical equipment and methods, culture process, tissue culture, etc., can solve problems such as adverse immune reactions, diseases or injuries, and achieve no immune rejection, simple treatment methods, and low cost Effect

Inactive Publication Date: 2019-10-11
广州瑞臻再生医学科技有限公司
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Potentially even causing disease or injury, stem cell therapy can also induce adverse immune responses

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • NPC culture medium, preparation method for exosome and application
  • NPC culture medium, preparation method for exosome and application
  • NPC culture medium, preparation method for exosome and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]An embodiment of NPC (neural progenitor cell) culture medium in the present invention includes DMEM / F12 medium, human transferrin (Human Transferrin), recombinant human insulin (Human Recombinant Insulin), progesterone (Progesterone), corticosteroids Corticosterone, T3 (triodo-I-thyronine), Glutathione, Catalase, Superoxide Dismutase, Vitamin E (DL Alpha Tocopherol Acetate), Natural vitamin E oil (tocopherol, DL Alpha-Tocopherol), linoleic acid (Linoleic Acid), lipoic acid (lipoic acid), linolenic acid (Linolenic Acid), L-carnitine hydrochloride (L-Carnitine HCl), rotten Amine (Putrescine 2HCl), Ethanolamine HCl, Sodium Selenite, D-Galactose, Lactic Acid, Sodium Pyruvate, Glutamine Amide (glutamax, Thermo Fisher Cell Culture Supplement), vitamin H (Biotin), lipidated bovine serum albumin (lipidated BSA), ascorbic acid vitamin C (AscorbicAcid), sodium chloride (NaCl), dorsomorphin, basic fibroblast Growth factor (bFGF) and insulin (insulin);

[0033] Calculated on the b...

Embodiment 2

[0039] A kind of embodiment of NPC (neural progenitor cell) culture medium of the present invention, compared with the NPC culture medium of embodiment 1, difference only lies in the content of each component in every 1L medium respectively:

[0040]

Embodiment 3

[0042] A kind of embodiment of NPC (neural progenitor cell) culture medium of the present invention, compared with the NPC culture medium of embodiment 1, difference only lies in the content of each component in every 1L medium respectively:

[0043]

[0044]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a neural progenitor cell culture medium. The neural progenitor cell culture medium comprises a DMEM / F12 culture medium, human transferrin, recombinant human insulin, progesterone, corticosterone, T3 (Triodo-I-thyronine), glutathione, catalase, superoxide dismutase, vitamin E acetate, tocopherol, linoleic acid, lipoic acid, linolenic acid, L-carnitine hydrochloride, putrescine, ethanolamine hydrochloride, sodium selenite, D-galactose, lactic acid, sodium pyruvate, glutamine, vitamin H, lipidated bovine serum albumin, vitamin C, sodium chloride, dorsomorphin, a basic fibroblast growth factor and insulin. The invention further provides a preparation method for an exosome and application of the exosome. The subcells (acells) of the exosome prepared by using the method have the advantages of being low in cost, safe, free of immunological rejection, capable of penetrating through a blood brain barrier, accurate in target point, simple in treatment means and the like,and the culture medium has a great potential in acellular regeneration medicine.

Description

technical field [0001] The invention relates to drugs for treating neurodegenerative diseases, in particular to a preparation method and application of NPC culture medium and exosomes. Background technique [0002] Neurodegenerative diseases, including Alzheimer's and Parkinson's, are currently treated primarily with drugs. Alzheimer's disease is a degenerative disease of the central nervous system of unknown cause, characterized by progressive memory impairment and loss of cognitive function accompanied by decreased ability of daily living and behavioral changes. The prevalence of Alzheimer's disease is 6.6% for those over 65 years old, and over 22% for those over 80 years old. According to the 2018 medical statistics report of the United States, Alzheimer's disease ranks sixth among the top ten deadly diseases. Ebison, Aricept, Exelon and Liyilin are currently the main drugs for the treatment of Alzheimer's disease. [0003] Parkinson's disease is the second most common...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0797A61K35/30A61P25/28
CPCA61K35/30A61P25/28C12N5/0618C12N5/0623C12N2500/12C12N2500/25C12N2500/30C12N2500/34C12N2500/38C12N2500/46C12N2501/115C12N2501/30C12N2501/39C12N2501/71C12N2501/998C12N2506/45
Inventor 张凌何翠敏
Owner 广州瑞臻再生医学科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com