NPC culture medium, preparation method for exosome and application

A culture medium and exosome technology, applied in biochemical equipment and methods, culture process, tissue culture, etc., can solve problems such as adverse immune reactions, diseases or injuries, and achieve no immune rejection, simple treatment methods, and low cost Effect

Inactive Publication Date: 2019-10-11
广州瑞臻再生医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Potentially even causing disease or injury, stem cell therapy can also induce adverse immune responses

Method used

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  • NPC culture medium, preparation method for exosome and application
  • NPC culture medium, preparation method for exosome and application
  • NPC culture medium, preparation method for exosome and application

Examples

Experimental program
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Embodiment 1

[0032]An embodiment of NPC (neural progenitor cell) culture medium in the present invention includes DMEM / F12 medium, human transferrin (Human Transferrin), recombinant human insulin (Human Recombinant Insulin), progesterone (Progesterone), corticosteroids Corticosterone, T3 (triodo-I-thyronine), Glutathione, Catalase, Superoxide Dismutase, Vitamin E (DL Alpha Tocopherol Acetate), Natural vitamin E oil (tocopherol, DL Alpha-Tocopherol), linoleic acid (Linoleic Acid), lipoic acid (lipoic acid), linolenic acid (Linolenic Acid), L-carnitine hydrochloride (L-Carnitine HCl), rotten Amine (Putrescine 2HCl), Ethanolamine HCl, Sodium Selenite, D-Galactose, Lactic Acid, Sodium Pyruvate, Glutamine Amide (glutamax, Thermo Fisher Cell Culture Supplement), vitamin H (Biotin), lipidated bovine serum albumin (lipidated BSA), ascorbic acid vitamin C (AscorbicAcid), sodium chloride (NaCl), dorsomorphin, basic fibroblast Growth factor (bFGF) and insulin (insulin);

[0033] Calculated on the b...

Embodiment 2

[0039] A kind of embodiment of NPC (neural progenitor cell) culture medium of the present invention, compared with the NPC culture medium of embodiment 1, difference only lies in the content of each component in every 1L medium respectively:

[0040]

Embodiment 3

[0042] A kind of embodiment of NPC (neural progenitor cell) culture medium of the present invention, compared with the NPC culture medium of embodiment 1, difference only lies in the content of each component in every 1L medium respectively:

[0043]

[0044]

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Abstract

The invention discloses a neural progenitor cell culture medium. The neural progenitor cell culture medium comprises a DMEM/F12 culture medium, human transferrin, recombinant human insulin, progesterone, corticosterone, T3 (Triodo-I-thyronine), glutathione, catalase, superoxide dismutase, vitamin E acetate, tocopherol, linoleic acid, lipoic acid, linolenic acid, L-carnitine hydrochloride, putrescine, ethanolamine hydrochloride, sodium selenite, D-galactose, lactic acid, sodium pyruvate, glutamine, vitamin H, lipidated bovine serum albumin, vitamin C, sodium chloride, dorsomorphin, a basic fibroblast growth factor and insulin. The invention further provides a preparation method for an exosome and application of the exosome. The subcells (acells) of the exosome prepared by using the method have the advantages of being low in cost, safe, free of immunological rejection, capable of penetrating through a blood brain barrier, accurate in target point, simple in treatment means and the like,and the culture medium has a great potential in acellular regeneration medicine.

Description

technical field [0001] The invention relates to drugs for treating neurodegenerative diseases, in particular to a preparation method and application of NPC culture medium and exosomes. Background technique [0002] Neurodegenerative diseases, including Alzheimer's and Parkinson's, are currently treated primarily with drugs. Alzheimer's disease is a degenerative disease of the central nervous system of unknown cause, characterized by progressive memory impairment and loss of cognitive function accompanied by decreased ability of daily living and behavioral changes. The prevalence of Alzheimer's disease is 6.6% for those over 65 years old, and over 22% for those over 80 years old. According to the 2018 medical statistics report of the United States, Alzheimer's disease ranks sixth among the top ten deadly diseases. Ebison, Aricept, Exelon and Liyilin are currently the main drugs for the treatment of Alzheimer's disease. [0003] Parkinson's disease is the second most common...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0797A61K35/30A61P25/28
CPCA61K35/30A61P25/28C12N5/0618C12N5/0623C12N2500/12C12N2500/25C12N2500/30C12N2500/34C12N2500/38C12N2500/46C12N2501/115C12N2501/30C12N2501/39C12N2501/71C12N2501/998C12N2506/45
Inventor 张凌何翠敏
Owner 广州瑞臻再生医学科技有限公司
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