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Functional comprehensive-element biological fertilizer, and preparation method thereof

A biological fertilizer and functional technology, applied in the preparation of organic fertilizers, animal carcass fertilizers, alkaline orthophosphate fertilizers, etc., can solve problems in the initial stage, reduce usage, improve product quality, and increase crop types Effect

Inactive Publication Date: 2019-10-15
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the utilization of most of the straw directly returned to the field and the creation of bio-fertilizers is still in its infancy

Method used

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  • Functional comprehensive-element biological fertilizer, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Step 1: Activate Aureobasidium pullulans in potato dextrose agar medium for 5 days at 28°C, activate Bacillus in LB medium for 37°C for 48 hours, and set aside.

[0032] Step 2: Mix the Aureobasidium pullulans and Bacillus that have been cultivated to the logarithmic growth phase at a ratio of 3:4, and set aside.

[0033] Step 3: Take the seed solution of the mixed bacteria system according to the inoculum size of 4% (v / w) and insert it into the fermentation medium (20g of straw, 5% of xylan, 3% of urea, KH2PO4 0.5%, VB2 0.01%, Distilled water 40mL, pH natural) Erlenmeyer flask, do 3 parallel experiments under every kind of same condition, place 28 ℃ and ferment culture 5d.

Embodiment 2

[0035] Step 1: Activate Aureobasidium pullulans in potato dextrose agar medium for 5 days at 28°C, activate Bacillus in LB medium for 37°C for 48 hours, and set aside.

[0036] Step 2: Mix the Aureobasidium pullulans and Bacillus that have been cultivated to the logarithmic growth phase at a ratio of 3:4, and set aside.

[0037] Step 3: Take the mixed bacteria system seed solution and insert it into the fermentation medium (crayfish processing waste 20g, xylan 5%, urea 3%, KH2PO4 0.5% by 4% (v / w) inoculation amount) , VB2 0.01%, distilled water 40mL, pH natural) in the Erlenmeyer flask, do 3 parallel experiments under each same condition, place 28 ℃ to ferment and cultivate 5d.

Embodiment 3

[0039] Step 1: Activate Aureobasidium pullulans in potato dextrose agar medium for 5 days at 28°C, activate Bacillus in LB medium for 37°C for 48 hours, and set aside.

[0040] Step 2: Mix the Aureobasidium pullulans and Bacillus that have been cultivated to the logarithmic growth phase at a ratio of 3:4, and set aside.

[0041] Step 3, fermentation: take the seed solution of the mixed bacteria system according to the inoculation amount of 10% by volume and mass, and insert it into the fermentation medium containing straw and the fermentation medium containing crayfish processing waste, and ferment at 28°C for 5 days to obtain ;

[0042] Step 4, inoculate the mixed bacteria system fermented in step 3 into the mixture of straw and crayfish processing waste, and ferment to prepare whole-element bio-organic fertilizer; wherein, 40Kg of the mixed bacteria system fermented in step 3 is added to each ton of the mixture; ferment The temperature is 28°C, and the fermentation time is ...

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Abstract

The invention discloses a functional comprehensive-element biological fertilizer, and a preparation method thereof. According to the preparation method, straw and crayfish processing waste are taken as main raw materials, and Aureobasidium pullulans and Bacillus spp are adopted for fermentation so as to obtain a finished product. The preparation method comprises following specific steps: 1, Aureobasidium pullulans is subjected to activation culture in potato glucose agar culture-medium for 5d, Bacillus spp is subjected to activation culture in LB culture medium for 24h; 2, the Aureobasidium pullulans and Bacillus spp cultured to be at logarithmic phase are mixed at a ratio of 2:5-5:2; 3, a straw-containing fermentation culture medium, and a crayfish processing waste containing fermentationculture medium are inoculated with, by volume, 4 to 20% of an obtained mixed bacterium seed solution respectively, and fermentation culture is carried out for 2 to 10d at 28 DEG C; and 4, after fermentation in step 3, a mixture of straw and crayfish processing waste is inoculated with a mixed bacterium system obtained in step 3, and fermentation is carried out to prepare the functional comprehensive-element biological fertilizer, wherein 40 to 100kg of the mixed bacterium system obtained in step 3 is added into each ton of the mixture of straw and crayfish processing waste.

Description

technical field [0001] The invention specifically relates to a functional whole-element biological fertilizer and a preparation method thereof. Background technique [0002] Biological fertilizer is made from crop straw, livestock and poultry manure, agricultural by-products and organic waste from food processing as raw materials, and fermented with multifunctional compound bacterial agents. Biological fertilizers not only contain a high proportion of organic matter, but also contain functional microorganisms that improve fertilizer or nutrient release capabilities. They can not only provide a variety of inorganic and organic nutrients to crops, but also fertilize the soil, store water and preserve fertilizers, and have quick-acting inorganic fertilizers. Sexuality and long-term effect of organic fertilizer. Existing studies have shown that plant hormones and cytokinins produced by plant symbiotic microorganisms are positively correlated with the good growth and development...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F15/00C05F17/00C05G1/00
CPCC05B7/00C05F1/005C05F17/00C05F11/00C05F11/08C05C3/00C05D3/00C05D9/02Y02W30/40
Inventor 余晓红商曰玲李凤伟王笃军张志义余学鹏沈丹蓉王海洋
Owner YANCHENG INST OF TECH
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