The invention discloses a functional comprehensive-element biological
fertilizer, and a preparation method thereof. According to the preparation method,
straw and
crayfish processing waste are taken as main raw materials, and
Aureobasidium pullulans and Bacillus spp are adopted for
fermentation so as to obtain a finished product. The preparation method comprises following specific steps: 1,
Aureobasidium pullulans is subjected to activation culture in potato glucose
agar culture-medium for 5d, Bacillus spp is subjected to activation culture in LB culture medium for 24h; 2, the
Aureobasidium pullulans and Bacillus spp cultured to be at logarithmic phase are mixed at a ratio of 2:5-5:2; 3, a
straw-containing
fermentation culture medium, and a
crayfish processing waste containing fermentationculture medium are inoculated with, by volume, 4 to 20% of an obtained mixed bacterium seed solution respectively, and
fermentation culture is carried out for 2 to 10d at 28 DEG C; and 4, after fermentation in step 3, a mixture of
straw and
crayfish processing waste is inoculated with a mixed bacterium
system obtained in step 3, and fermentation is carried out to prepare the functional comprehensive-element biological
fertilizer, wherein 40 to 100kg of the mixed bacterium
system obtained in step 3 is added into each
ton of the mixture of straw and crayfish processing waste.