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Preparation method of cervus and cucumis polypeptide injection preparation

A technology for injection preparations and deer melon polypeptides, applied in the field of deer melon polypeptide injection preparations, can solve the problems of reduced active ingredient content, increased clinical allergic reactions, and reduced bioavailability, and achieves low product cost, excellent quality, and reduced medication costs. Effect

Inactive Publication Date: 2019-10-18
哈尔滨誉衡制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the peptides in Lugua Polypeptide Injection are easy to polymerize into macromolecules, and the alkali and tannins in it can accelerate this polymerization reaction, so Lugua Polypeptide Injection is easy to polymerize during storage, leading to increased clinical allergic reactions. It is not conducive to long-term storage; the freeze-dried powder injection of Lugua polypeptide also has poor stability during long-term storage, resulting in a decrease in the content of active ingredients and a decrease in bioavailability, which affects clinical application

Method used

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  • Preparation method of cervus and cucumis polypeptide injection preparation
  • Preparation method of cervus and cucumis polypeptide injection preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take the deer bone, remove the bone marrow, crush, weigh, add 1.5% deer bone weight compound protease and 2 times deer bone weight water for injection, compound protease is pepsin and papain with a weight ratio of 1.85:1; extract at 40°C for 3 hours, Filtrate, adjust the pH of the filtrate to 4.5 with citric acid, let stand, filter, adjust the pH to 8.5 with sodium citrate, add 0.1 times the weight of deer bone pancreatin, hydrolyze at 40°C for 3 hours, filter, and citrate the filtrate The pH value is adjusted by acid to 6.5, followed by an ultrafiltration system with a molecular weight cut-off of 100,000, a molecular weight cut-off ultrafiltration system with a molecular weight of 10,000, and a molecular weight cut-off ultrafiltration unit with a molecular weight of 8,000 to perform ultrafiltration, retain the permeate, and freeze-dry to obtain peptides extracted from deer bone .

[0025] The preparation method of the polypeptide extracted from melon seeds is:

[0026] Tak...

Embodiment 2

[0029] The preparation method of the polypeptide extracted from deer bone is: take deer bone, remove bone marrow, crush, weigh, add 2.5% deer bone weight compound protease and 4 times deer bone weight water for injection, compound protease is a pepsin and a weight ratio of 2.35:1 Papain: Extract for 1 hour at 50℃, filter, adjust the pH of the filtrate to 6.0 with citric acid, let stand, filter, adjust the pH to 9.0 with sodium citrate for the filtrate, add 0.3 times the weight of deer bone pancreatin, and hydrolyze at 50℃ Filter for 1 hour, adjust the filtrate to pH 7.5 with citric acid, and use the ultrafiltration system with a molecular weight cut-off of 100,000, an ultrafiltration system with a molecular weight of 10,000, and an ultrafiltration unit with a molecular weight of 8,000 to perform ultrafiltration and retain the permeate. , Freeze-drying, get the polypeptide extracted from deer bone.

[0030] The preparation method of the polypeptide extracted from melon seeds is: t...

Embodiment 3

[0033] The preparation method of polypeptide extracted from deer bone is: take deer bone, remove bone marrow, crush, weigh, add 2.0% deer bone weight compound protease and 3 times deer bone weight water for injection, compound protease is pepsin and weight ratio 2.05:1 Papain; extract for 1 to 3 hours at 40℃~50℃, filter, adjust the pH of the filtrate to 5.5 with citric acid, let stand, filter, adjust the pH of the filtrate to 8.8 with sodium citrate, add 0.2 times the weight of deer bone pancreas Enzyme, hydrolyze at 45°C for 2 hours, filter, adjust the filtrate to pH 7.0 with citric acid, and use the ultrafiltration system with a molecular weight cut-off of 100,000, a molecular weight cut-off ultrafiltration system with a molecular weight of 10,000, and a molecular weight cut-off ultrafiltration unit to perform ultrafiltration. , Retain the permeate, freeze-dry, and get the polypeptide extracted from deer bone.

[0034] The preparation method of the polypeptide extracted from me...

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Abstract

The invention belongs to the technical field of biological medicine, and more specifically relates to a preparation method of a cervus and cucumis polypeptide injection preparation. The injection is prepared from a polypeptide extracted from deer bone, a polypeptide extracted from melon seeds, and sodium alginate; the research shows that the injection preparation has simple process, is suitable for industrial production, removes activated carbon process method, and meets the requirements of consistency evaluation; the sodium alginate is used as an auxiliary material for the preparation to ensure the stability of the preparation product, and further, a product with better quality can be obtained. The injection preparation of the invention meets the requirements of the conventional preparation of the injection preparation, such as endotoxin, difference in the amount of the preparation, and the like, and has higher potential safety; the dosage of the preparation auxiliary materials is small, the product cost is lower, and the medication cost of the patient is lowered. Through the stability test for the preparation of the invention, the polypeptide content, the free amino acid content,and the high molecular substance content are hardly increased or increased, and the safety is better.

Description

Technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a deer melon polypeptide injection preparation. Background technique [0002] Deer Melon Polypeptides are made from the bones of the deer family animal Sika deer and the dried mature seeds of the Cucurbitaceae plant muskmelon. They mainly contain osteoinductive polypeptide biological factors, melon seed extracts, a variety of free amino acids, organic calcium, phosphorus, etc. ingredient. [0003] The medicinal preparations using deer bone and melon seeds as raw materials are mainly deer melon polypeptide injection preparations, which have the functions of regulating bone metabolism, stimulating the proliferation of osteoblasts, and promoting new bone formation. It can also regulate calcium scale metabolism and increase bone calcium deposition. , Prevent osteoporosis, and has anti-inflammatory, analgesic, anti-rheumatic effects, and has been widely used in clinical pract...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61K47/36A61K9/08A61P19/08A61P19/10A61P29/00A61P19/02A61P19/04C12P21/06C07K1/34
CPCA61K38/012A61K38/011A61K47/36A61K9/0019A61K9/08A61P19/08A61P19/10A61P29/00A61P19/02A61P19/04C12P21/06A61K2300/00
Inventor 朱吉满何利群夏瑞雪宁夏王帆
Owner 哈尔滨誉衡制药有限公司
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