A new strain of Streptomyces producing daptomycin and its application

A technology of daptomycin and streptomyces, applied in the field of microorganisms, can solve the problems of complex secondary metabolites, difficulty in separating and purifying daptomycin, accumulation of by-product pigments, etc., achieve good growth conditions, convenient and direct microscopic examination , The effect of reducing the consumption level of carbon and nitrogen sources
CN110343638BActive Publication Date: 2020-08-11ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2020-08-11

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Abstract

The invention provides a new strain of daptomycin-producing Streptomyces and its application, which is a subspecies of Streptomyces griseus, which is named Streptomyces griseus L340 (Streptomyces griseus), and its preservation number is CGMCC17921. The genetic character and fermentation unit of the strain provided by the invention are relatively stable, the culture and fermentation conditions are suitable for industrial production of daptomycin, and the pigment interference in the later separation and purification is eliminated. The Streptomyces provided by the present invention obtains the secondary metabolite daptomycin through fermentation, which has the advantages of no interference from pigment accumulation, cleaner metabolic profile, and obvious advantages in later stage separation and purification.
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Description

technical field

[0001] The invention belongs to the field of microorganisms, and relates to a new daptomycin-producing streptomyces strain and application thereof. Background technique

[0002] Daptomycin is a cyclolipopeptide antibiotic with a new structure extracted from the fermentation broth of S. reseosporus. It was discovered by Eli Lilly and Company in the 1980s and developed by Cubist Pharmaceuticals in 1997. Successful, it not only has a novel chemical structure, but also its mode of action is different from any approved antibiotic: it hinders the biosynthesis of bacterial cell wall peptidoglycan by disrupting the transport of amino acids by the cell membrane, and changes the cytoplasmic membrane. It can destroy the function of bacterial cell membrane in many ways, and quickly kill Gram-positive bacteria. In addition to its ability to act on most clinically relevant Gram-positive bacteria, daptomycin is more important for isolates that have shown resistance to meth...

Claims

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