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Fabrication method of PbS/Co3O4 compound signal reduction-type photoelectric chemical immunosensor

An immune sensor, photoelectrochemical technology, applied in scientific instruments, instruments, material analysis through electromagnetic means, etc., can solve the problems of limited application, achieve the effect of improving detection sensitivity, increasing roughness, and accelerating electron transfer rate

Active Publication Date: 2019-10-18
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a photoelectric conversion semiconductor material with excellent performance, titanium dioxide is widely used in photocatalysis, fuel cells and other fields, but its wide band gap (3.2 eV) makes it only absorb ultraviolet light and limits its application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 A kind of based on PbS / Co 3 o 4 The preparation method of the photoelectrochemical procalcitonin sensor of the composite, the preparation steps are as follows:

[0044] 1) Cut a large piece of ITO conductive glass into 2.0 cm × 0.8 cm, ultrasonically clean it with acetone, ethanol and ultrapure water for 30 min, and blow dry with nitrogen;

[0045] 2) Add 10 µL, 5 mg / mL B-TiO dropwise 2 NPs evenly disperse the suspension to the conductive surface of ITO conductive glass, and dry it at room temperature to obtain B-TiO 2 NPs electrode;

[0046] 3) B-TiO 2 NPs electrodes were in 5 mM Bi(NO) 3 and 5 mM KI solution for 10 s, rinsed with ultrapure water, and repeated the cycle 20 times to obtain B-TiO 2 NPs / BiOI NSs electrodes;

[0047] 4) Add 8 µL of Au NPs solution to B-TiO 2 NPs / BiOI NSs electrode surface, prepared B-TiO 2 NPs / BiOINSs / Au NPs electrodes;

[0048] 5) Drop 5 µL, 8 µg / mL procalcitonin capture antibody solution onto the surface of t...

Embodiment 2

[0052] Embodiment 2 A kind of based on PbS / Co 3 o 4 The preparation method of the photoelectrochemical procalcitonin sensor of the composite, the preparation steps are as follows:

[0053] 1) Cut a large piece of ITO conductive glass into 2.0 cm × 0.8 cm, ultrasonically clean it with acetone, ethanol and ultrapure water for 30 min, and blow dry with nitrogen;

[0054] 2) Add 10 µL, 7 mg / mL B-TiO dropwise 2 NPs evenly disperse the suspension to the conductive surface of ITO conductive glass, and dry it at room temperature to obtain B-TiO 2 NPs electrode;

[0055] 3) B-TiO 2 NPs electrodes were in 8 mM Bi(NO) 3 and 8 mM KI solution for 10 s, rinsed with ultrapure water, and repeated the cycle 25 times to obtain B-TiO 2 NPs / BiOI NSs electrodes;

[0056] 4) Add 8 µL of Au NPs solution to B-TiO 2 NPs / BiOI NSs electrode surface, prepared B-TiO 2 NPs / BiOINSs / Au NPs electrodes;

[0057] 5) Drop 5 µL, 10 µg / mL procalcitonin capture antibody solution onto the surface of ...

Embodiment 3

[0061] Example 3 B-TiO 2 The preparation of NPs, the steps are as follows:

[0062] 4.0 g P25 titania nanoparticles and 1.5 g NaBH 4 Mixed and ground for 30 min, calcined at 300 °C for 50 min in an argon atmosphere tube furnace, cooled to room temperature, centrifuged with ultrapure water and ethanol for 3 times, and placed in a vacuum oven at 35 °C for 12 h to obtain B-TiO 2 NPs.

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Abstract

The invention relates to fabrication method and application of a lead sulfide-cobalt oxide compound (PbS / Co3O4)-based photoelectric chemical procalcitonin sensor. Black titanium dioxide nanoparticle (B-TiO2 NPs), iodine oxygen bismuth nanosheet (BiOI NSs) and gold nanoparticle (Au NPs) are combined on indium tin oxide (ITO) conductive glass and are used as a substrate material, the light absorption range can be expanded by a formed sensitization structure, the photoproduction electron hole separation efficiency is facilitated, and meanwhile, the sensor is used for loading an antibody. The fabricated PbS / Co3O4 compound competes for a light source and a hole sacrifice agent with the substrate material, the signal reduction-type photoelectric chemical immunosensor is built, sensitive detection of the procalcitonin is achieved, and the method is of important significance to early diagnosis and monitoring of bacteria inflammatory disease infection.

Description

technical field [0001] The invention belongs to the technical field of nano functional materials, immune analysis and biosensing, and specifically combines black titanium dioxide nanoparticles, bismuth oxyiodine nanosheets and gold nanoparticles (B-TiO 2 NPs / BiOI NSs / Au NPs) as substrate material, PbS / Co 3 o 4 As a signal antibody marker, a photoelectrochemical immunosensor for detecting procalcitonin was constructed. Background technique [0002] Procalcitonin (PCT), the natural precursor of calcitonin, produced by thyroid C cells and proteolytically, is a promising disease marker for the diagnosis of bacterial infections that outperforms other known disease markers The diagnosis of sepsis is more sensitive. The content of procalcitonin in normal people is less than 0.1 ng / mL, but when bacterial infection or sepsis occurs, the content of procalcitonin will increase rapidly, and the amount of increase depends on the degree of infection. Therefore, the early diagnosis of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 钱彦荣魏琴冯金慧王欢刘蕾刘雪静冯瑞卿鞠熀先
Owner UNIV OF JINAN
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