Fluorescent carbon dot nano-probe for detecting hydrogen sulfide and imaging hydrogen sulfide in living cells based on inner filter effect, and using method thereof

A technology of fluorescent carbon dots and nano-probes, which is applied in the field of biochemical analysis, can solve the problems such as the inability to continuously detect the content of hydrogen sulfide, and achieve the effect of high selectivity and low cost

Active Publication Date: 2019-10-22
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing fluorescent probes can only detect whether the sample to be tested contai

Method used

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  • Fluorescent carbon dot nano-probe for detecting hydrogen sulfide and imaging hydrogen sulfide in living cells based on inner filter effect, and using method thereof
  • Fluorescent carbon dot nano-probe for detecting hydrogen sulfide and imaging hydrogen sulfide in living cells based on inner filter effect, and using method thereof
  • Fluorescent carbon dot nano-probe for detecting hydrogen sulfide and imaging hydrogen sulfide in living cells based on inner filter effect, and using method thereof

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Experimental program
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Effect test

Embodiment 1

[0033] Preparation method of fluorescent carbon dot nanoprobe for detection of hydrogen sulfide and intracellular hydrogen sulfide imaging based on inner filter effect:

[0034] 1. Synthesis of Fluorescent Carbon Nanodots

[0035] Fluorescent carbon nanodots (CDs) were prepared by pyrolyzing tomatoes, as follows: Weigh 5.0 g of fresh tomatoes in a mortar, grind them fully, filter them through a 150-mesh filter, take 3 mL of the filtrate into a hydrothermal reaction kettle, and add 20 mL of ultrapure water was heated to 250 °C and stirred. After 10 hours, the heating was stopped and the solution was naturally cooled to room temperature. After filtration, the filtrate was collected in a dialysis bag (MWCO: 1 kDa, poresize: ca. 1.0 nm) in an ultrapure water environment. After photodialysis for 24 h, the supernatant was taken and stored at 4°C in the dark. figure 1 The TEM images and particle size distribution diagrams of the synthesized green carbon quantum dots are given. It ca...

Embodiment 2

[0043] 1. Synthesis of Fluorescent Carbon Nanodots

[0044] Fluorescent carbon nanodots (CDs) were prepared by pyrolyzing tomatoes, as follows: Weigh 5.0 g of fresh tomatoes in a mortar, grind them fully, filter them through a 150-mesh filter, take 3 mL of the filtrate into a hydrothermal reaction kettle, and add 21 mL of ultrapure water was heated to 250 °C and stirred. After 10 hours, the heating was stopped and the solution was naturally cooled to room temperature, filtered, and the filtrate was taken in a dialysis bag (MWCO: 1 kDa, poresize: ca. 1.0 nm) in an ultrapure water environment. After photodialysis for 24 h, the supernatant was taken and stored at 4°C in the dark.

[0045] 2. Synthesis of DMI

[0046] 2,3,3-Trimethyl-3H-indole (1.6g, 10mmol) and methyl iodide (2.84g, 20mmol) were mixed and dissolved in 10mL of acetonitrile, refluxed at 60°C for 11h under argon protection, and cooled to room temperature naturally , a light pink precipitate was obtained, which was...

Embodiment 3

[0052] 1. Synthesis of Fluorescent Carbon Nanodots

[0053] Fluorescent carbon nanodots (CDs) were prepared by pyrolyzing tomatoes, as follows: Weigh 5.0 g of fresh tomatoes in a mortar, grind them fully, filter them through a 150-mesh filter, take 3 mL of the filtrate into a hydrothermal reaction kettle, and add 22 mL of ultrapure water was heated to 250 °C and stirred. After 10 hours, the heating was stopped and the solution was naturally cooled to room temperature, filtered, and the filtrate was taken in a dialysis bag (MWCO: 1 kDa, poresize: ca. 1.0 nm) in an ultrapure water environment. After photodialysis for 24 h, the supernatant was taken and stored at 4°C in the dark.

[0054] 2. Synthesis of DMI

[0055] 2,3,3-Trimethyl-3H-indole (4.8g, 30mmol) and iodomethane (8.52g, 60mmol) were mixed and dissolved in 10mL of acetonitrile, refluxed at 60°C for 11h under argon protection, and cooled to room temperature naturally , a light pink precipitate was obtained, which was f...

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Abstract

The invention proposes a fluorescent carbon dot nano-probe for detecting hydrogen sulfide and imaging hydrogen sulfide in living cells based on the inner filter effect, and a using method thereof. A simple green method for fluorescence detection of hydrogen sulfide and tracing of hydrogen sulfide in living cells is established. Fluorescent carbon dots are prepared from tomatoes, the carbon dots (CDs) have strong green fluorescence at 520 nm under the irradiation of 410 nm ultraviolet light, red shift of the fluorescence emission peak gradually occurs with the increase of the wavelength of theexcitation light, the relative fluorescence quantum yield is 53%, the carbon dots acting as a fluorescence donor and an organic small-molecular receptor (DMI) form the probe based on the inner filtereffect, and the probe is used for detecting exogenous and endogenous hydrogen sulfide. A sensing system is successfully applied to hydrogen sulfide detection and live cell imaging analysis as the fluorescent probe, and a sensing method has broad application prospects in the fields of environmental analysis, biochemical analysis and cell imaging analysis.

Description

technical field [0001] The invention relates to the technical field of biochemical analysis, in particular to a fluorescent carbon dot nanoprobe for detecting hydrogen sulfide and imaging hydrogen sulfide in living cells based on an inner filter effect, and a method for using the same. Background technique [0002] Carbon quantum dots (CDs), also known as carbon dots or carbon nanodots, are a new type of carbon nanomaterials, generally below 10 nm in size, with excellent optoelectronic properties, uniform particles, good dispersibility and low preparation cost. The toxicity, good biocompatibility, adjustable excitation and emission wavelengths, good photostability, no light flicker phenomenon and so on. Compared with traditional metal quantum dots, CDs have many advantages, and their unique luminescence properties and biocompatibility have good application prospects in the fields of light-emitting devices, photocatalysis, biochemical analysis, optoelectronics, cell imaging a...

Claims

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Application Information

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IPC IPC(8): C09K11/65C09K11/02G01N21/64
CPCC09K11/025C09K11/65G01N21/64
Inventor 张旭陈云云方东张超杨婷周航
Owner HENAN UNIVERSITY
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