Method for establishing intestinal health state diagnosis model based on fat-soluble metabolite factor in serum and application

Abstract

The invention relates to a method for establishing an intestinal health state diagnosis model based on a fat-soluble metabolite factor in serum and an application. The diagnosis model is established by using a feedforward back propagation neural network algorithm, a joint factor is determined, and a screening method for the construction of the fat-soluble metabolite factor in the intestinal healthstate diagnosis model is proposed. The method of the invention comprises the steps of establishing a liquid phase tandem mass spectrometry combined detection method for the simultaneous detection ofthe content of 110 lipid-soluble metabolites in a human serum, ie, screening for 110 lipid-soluble metabolites with better peak shapes, and then performing serum-targeted fat-soluble metabolite metabolomics study to determine a best differential fat-soluble metabolite as the fat-soluble metabolite factor. The intestinal health state diagnosis model constructed in the invention provides an effective and reliable method for the clinical diagnosis of intestinal health and has a good value of intestinal health assisted diagnosis.

Description

Technical field [0001] The invention belongs to the technical field of intestinal health diagnosis, and particularly relates to a method for establishing an intestinal health status diagnosis model based on fat-soluble metabolite factors in serum and its application. Background technique [0002] Intestinal health, especially colorectal cancer, is a common cancer that occurs in the gastrointestinal tract. The cause of the disease is mostly related to poor living habits and aging. The risk of disease in people over 40 has risen sharply. Colorectal cancer has the third highest incidence and second mortality among global cancers. In China, the incidence of colorectal cancer ranks third among cancers, and the mortality rate ranks fifth. Colorectal cancer is a relatively slow-developing cancer. It may take ten years to develop from early stage to stage IV. The 5-year survival rate of stage 0 / I colorectal cancer can reach 90%, while the survival rate of stage IV is only 5-7%. . Earl...

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Problems solved by technology

[0015] The third category is similar to the present invention in that the detection methods are similar, but there is no application for specific tumors, such as:

[0018] 1. For the third category of applications, although the detection methods are similar, the detected metabolites may overlap. The third category of applications is to patent the detection method, and then must rely on the use of all the information of all selected metabolites. Differentiate and identify different disease states, the detection method itself is the method and data published by the instrument manufacturer, and the creativity is not good;

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