Preparation and application of a nano-immune preparation based on porous calcium carbonate

A technology of porous calcium carbonate and immune preparations, which can be applied to medical preparations without active ingredients, medical preparations containing active ingredients, and active ingredients of aluminum/calcium/magnesium, etc. Failure, limited immune response, etc., to achieve high immune activation efficiency, eliminate small lesions, and improve the efficiency of immunotherapy

Active Publication Date: 2022-05-27
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have shown that the commonly used immune vaccine method has the following problems: first, the immune response effect caused by the immune vaccine is limited, generally need to add immune adjuvant to strengthen the immune response, but the toxicity of immune adjuvant (such as aluminum hydroxide) is relatively large
And the immunosuppressive microenvironment in the tumor tissue is usually difficult to break, which can easily lead to the failure of tumor immunotherapy

Method used

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  • Preparation and application of a nano-immune preparation based on porous calcium carbonate
  • Preparation and application of a nano-immune preparation based on porous calcium carbonate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 is based on the preparation of porous calcium carbonate nano-formulations

[0031] (1) Synthesis of porous CaCO by gas diffusion reaction 3 Nanoparticles: 220 mg CaCl 2 · H 2 O and 8 g NH 4 HCO 3 CaCO obtained after simultaneously placing in a vacuum drying chamber at a control temperature of 25 ° C and keeping the entire system in a vacuum environment for 24 hours 3 The nanoparticles are separated by centrifugation at 12,000 rpm. CaCO will then be in absolute ethanol 3 The hole is loaded with IDOi to get CaCO 3 @IDOi (abbreviated as CaI).

[0032] (2) Modify the above nanoparticles with liposomes. First, an ethanol solution of 20 mg of CaI is mixed with 2 mg of DOPA. DPC, cholesterol and DSPE-PEG are then added to the above solution in a 4:4:2M ratio, the solvent is dried with a rotary evaporator, and the resulting nanoparticles are dissolved in an aqueous solution for further use. The obtained nanoparticles are then stirred with 200 mg PEI to prepare CaCO 3 @IDO...

Embodiment 2

[0036] Example 2 mouse molding experiment

[0037] 2.1 In vivo testing

[0038]Female Balb / c mice were selected as the research object, and a tumor-bearing mouse model was prepared by subcutaneous injection of mouse breast cancer cells, and the tumor was grown to 50 mm 3 Start the administration. The dosing groups were CaIPC (experimental group), LIPC, and normal saline. Tumor-bearing mice are administered intravenously, and tumor volume and weighing are measured every other day during the experimental cycle. The experimental results show that the CaIPC-treated mouse tumor growth rate in Example 1 is the slowest, indicating that the present invention can effectively inhibit tumor growth in a mouse model. Conversely, compared with the saline group, the LIPC group showed not very good tumor suppression effect, the results show that only the group treated with the present invention showed the optimal tumor treatment effect. Body weight is an important parameter to assess the systemic...

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Abstract

The invention belongs to the field of pharmaceutical preparations, in particular to a nano-immune preparation based on porous calcium carbonate, which uses porous CaCO 3 The nanoparticle is the carrier, and the inside of the hole is loaded with the immune blocking inhibitor IDOi, covered by the liposome layer, and the surface of the liposome layer carries the immune regulation nucleic acid CpG ODNs. The nano-immune preparation synthesized by the invention has good biocompatibility and high safety, has a good effect on treating breast cancer in mice and preventing cancer recurrence, the preparation process is simple and easy to realize, and has good application in the field of anti-tumor drug preparation. prospect.

Description

Technical field [0001] The present invention belongs to the field of pharmaceutical formulations, specifically relates to a nano immune formulation, to porous CaCO 3 Nanoparticles are carriers and loaded with immunoblocking inhibitor IDOi inside their pores, carrying the immunomodulatory nucleic acid CpG ODNs on the surface, their synthesis methods and their applications in the field of enhancing breast cancer efficacy and preventing its recurrence. Background [0002] Cancer is a malignant tumor that seriously threatens human health, and recurrence after treatment of primary tumors is the most important cause of death in most cancer patients. At present, the clinical and conventional treatment methods of tumors are mainly surgery, radiotherapy and chemotherapy, but after the treatment of these treatment methods, the body will still retain cancer cells (that is, tiny lesions), and these tiny lesions are the most important reasons for cancer recurrence. Therefore, the development ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K31/7105A61K45/00A61K33/10A61P35/00A61P37/04
CPCA61K31/7105A61K47/6915A61K47/6949A61K45/00A61P35/00A61P37/04A61K33/10A61K2300/00
Inventor 唐波李艳华李娜潘伟
Owner SHANDONG NORMAL UNIV
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