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A method for extracting and separating pigment from purple lucidum waste

A waste and pigment technology, applied in food extraction, function of food ingredients, chemical instruments and methods, etc., can solve the problems of inability to meet the quality requirements of food or health food, difficult dissolution of purple zhi pigment complex, and high cost. The effect of simple and easy extraction and separation methods

Active Publication Date: 2021-08-31
福建仙芝楼生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the cell wall of Zizhi fruiting body is composed of cellulose and lignin, which is very tough, the Zizhi pigment complex is difficult to dissolve and the extraction is incomplete.
[0004] The purple lucidum pigment complex is soluble in polar and weak polar solutions such as water or ethanol, and easily soluble in alkaline solutions; CN201010572833.7 "Extraction and preparation method of Ganoderma lucidum heteromelanin and its use" uses industrial ethanol for degreasing, acid extraction, and alkali extraction , acid hydrolysis, organic solvent washing, water washing and other steps to obtain Ganoderma lucidum heteromelanin, which is used to prepare medicines: its disadvantages are 1), the raw material used is Ganoderma lucidum fruiting body, and the process steps are loaded down with trivial details, resulting in high cost; 2), the obtained heteromelanin is pure Too high, insoluble in water and common organic reagents such as ethanol, and also insoluble in vegetable oil, resulting in difficulty in utilization; 3), some reagents used such as industrial ethanol and chloroform do not belong to the food additives stipulated by the state (GB 2760-2014 National Food Safety Standard Food Additive Use Standards), cannot guarantee the safety of ganoderma lucidum isomelanin, and did not provide the test results of solvent residues; these factors lead to the narrow application range of ganoderma lucidum isomelanin, which cannot meet the quality requirements of food or health food, and cannot be used in food or health food

Method used

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  • A method for extracting and separating pigment from purple lucidum waste
  • A method for extracting and separating pigment from purple lucidum waste
  • A method for extracting and separating pigment from purple lucidum waste

Examples

Experimental program
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Effect test

specific Embodiment 1

[0036] 1. 5 kg of the waste extracted from the fruiting body of Zizhi lucidum was subjected to steam explosion pretreatment to obtain explosives. The pressure during steam explosion was 2Mpa, and the treatment time was 600s;

[0037] 2 The explosives are extracted with 100kg of alkali-alcoholic solution, the alkali is food additive sodium hydroxide or potassium hydroxide, the solvent is 20% ethanol solution prepared by 95% edible grade ethanol and purified water, the final pH of the alkali-alcohol solution is 10, and the extraction temperature is 50°C, the extraction time is 2 hours; the filter mesh is 200 mesh, and about 100kg of alkali extraction solution is obtained by filtration;

[0038] 3 Adjust the pH of the alkali extraction solution to neutral with food additive hydrochloric acid, the final pH is 6, centrifuge to separate the solid phase and the liquid phase, the separation factor is 10,000 during centrifugation, the turbidity of the liquid phase is below 100NTU, and t...

specific Embodiment 2

[0043] 1. 5kg of the wastes extracted from the fruiting body of Zizhi lucidum were subjected to steam explosion pretreatment to obtain explosives. The pressure during steam explosion was 3Mpa, and the treatment time was 450s;

[0044] 2 The explosives are extracted with 100kg of alkali-alcoholic solution, the alkali is food additive sodium hydroxide or potassium hydroxide, the solvent is 20% ethanol solution prepared by 95% edible grade ethanol and purified water, the final pH of the alkali-alcohol solution is 10, and the extraction temperature is 55°C, the extraction time is 1.5h; the filter mesh is 200 mesh, and about 100kg of alkali extraction solution is obtained by filtration;

[0045] 3 Adjust the pH of the alkali extraction solution to neutral with food additive hydrochloric acid, the final pH is 6, centrifuge to separate the solid phase and the liquid phase, the separation factor is 10,000 during centrifugation, the turbidity of the liquid phase is below 100NTU, and the...

Embodiment 3

[0049] 1. 5 kg of the waste extracted from the fruiting body of Zizhi lucidum was subjected to steam explosion pretreatment to obtain explosives. The pressure during steam explosion was 3.5Mpa, and the treatment time was 300s;

[0050] 2 The explosives are extracted with 100kg of alkali-alcoholic solution, the alkali is food additive sodium hydroxide or potassium hydroxide, the solvent is 20% ethanol solution prepared by 95% edible grade ethanol and purified water, the final pH of the alkali-alcohol solution is 10, and the extraction temperature is 60°C, the extraction time is 1h; the filter mesh is 200 mesh, and about 100kg of alkali extraction solution is obtained by filtration;

[0051] 3. Adjust the pH of the alkali extraction solution to neutral with food additive hydrochloric acid, and the final pH is 7. Centrifuge to obtain a solid phase and a liquid phase. The separation factor during centrifugation is 10,000, and the turbidity of the liquid phase is below 100 NTU. The ...

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Abstract

The present invention relates to a method for extracting and separating pigments from Zizhi wastes: extract polysaccharides from Zizhi fruiting bodies with water, pretreat with steam explosion, extract with alkali-alcohol solution, filter, adjust the pH of the filtrate to neutral, Centrifuge to obtain the solid phase, that is, the alkali-soluble pigment extract; the liquid phase is desalted, dehydrated and concentrated by nanofiltration, added ethanol to dissolve, and centrifuged to obtain the solid phase, that is, the water-soluble pigment extract, and the liquid phase is recovered to obtain the alcohol-soluble pigment extract. This method utilizes the waste after extracting polysaccharides from Zizhi water to obtain three kinds of natural pigment extracts with different properties in Zizhi. The cost is very low, simple and easy to implement, and there is no environmental pollution. The prepared natural pigment extracts can be used in the fields of food and health products , together with Ganoderma lucidum extract can add color and flavor to the product.

Description

technical field [0001] The invention belongs to the field of natural product extraction and processing of agricultural by-products, relates to the technical field of processing and utilization of waste after extraction of Zizhi lucidum, and specifically relates to a method for extracting and separating Zizhi pigment from the waste produced after the polysaccharide is extracted from Zizhi water. Background technique [0002] Zizhi (Ganoderma sinense Zhao, Xu et Zhang) is a species of Ganoderma lucidum in the polyporaceae fungus (Chinese Pharmacopoeia 2015 edition one), sweet in taste and flat in nature, strengthening the body and strengthening the body. The main active ingredient is Ganoderma polysaccharide. At present, most of the utilization methods of Zizhi are traditional eating methods such as boiling and decocting, and modern processing methods such as direct crushing into powder or water extraction of Zizhi polysaccharides. It is then used as fuel for stoves. [0003]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C09B67/54A23L5/43
CPCA23V2002/00A23L5/43C09B61/00C09B67/0096A23V2200/044A23V2250/208A23V2300/14
Inventor 周岩飞陈桂秋林真李晔吴长辉姚渭溪
Owner 福建仙芝楼生物科技有限公司