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A kind of germination medium and germination method of chlamydospores of rhizoctonia rubrum

A germination medium and a technology of chlamydospores, which are applied in the field of plant pathogenic fungi research, can solve the problems of less research on chlamydospore germination and difficult germination of chlamydospores, and achieve convenient materials, strong operability, and simple ingredients Effect

Active Publication Date: 2021-04-20
INST OF PLANT PROTECTION FAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The conidia and chlamydospores of Verrucospora have pathogenic effects, and the conidia are very easy to germinate, while the chlamydospores are not easy to germinate. The germination rate of the chlamydospores of Verrucospora harmful on PDA medium is about 3% , while less research has been done on the germination of chlamydospores

Method used

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  • A kind of germination medium and germination method of chlamydospores of rhizoctonia rubrum
  • A kind of germination medium and germination method of chlamydospores of rhizoctonia rubrum
  • A kind of germination medium and germination method of chlamydospores of rhizoctonia rubrum

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Effect test

Embodiment 1

[0015] Embodiment 1: the influence of temperature on breaking the dormancy of rhizoma chlamydospores

[0016] (1) Transfer the test strain stored on the filter paper to a PDA medium plate, culture it at 28°C for 5 days, then transfer it to a new PDA medium plate, and cultivate it at 28°C for 10 days. Bacterial water Brush the chlamydospores of Rhizoctonia rubrum on the surface of the potato glucose medium, filter, and prepare 1.0×10 5 spores / mL of chlamydospore suspension, set aside.

[0017] (2) Preparation of Agaricus bisporus decoction medium (MuDA medium): Slice 200 g of Agaricus bisporus, add 1000 mL of water to boil for 30 min, filter with double gauze, add 16 g of agar powder to the supernatant, and heat to agar Dissolve the powder completely, add water to make up to 1000 mL, sterilize at 121°C for 25 min, and adjust the pH value to 7.0.

[0018] (3) Take 10 mL of the decoction medium of Agaricus bisporus for testing and pour it into a 9 cm petri dish to make a medium...

Embodiment 2

[0023] Embodiment 2: The influence of pH on the germination of rhizoctonia chlamydospores

[0024] (1) Transfer the test strain stored on the filter paper to a PDA medium plate, culture it at 28°C for 5 days, then transfer it to a new PDA medium plate, and cultivate it at 28°C for 10 days. Bacterial water Brush the chlamydospores of Rhizoctonia rubrum on the surface of the potato glucose medium, filter, and prepare 1 × 10 5 spores / mL of chlamydospore suspension, set aside.

[0025] (2) Use 0.1 mol / L hydrochloric acid solution and 0.1 mol / L sodium hydroxide solution to adjust the pH value of Agaricus bisporus decoction medium to 3, 4, 5, 6, 7, 8, 9 and 10, take Pour 10 mL of Agaricus bisporus decoction medium into a 9 cm petri dish to make a medium plate, and take 0.5 mL of the chlamydospore suspension of Rhizoctonia rubrum and evenly spread it on the Agaricus bisporus decoction medium plate above, air-dried the excess water, cultured in the dark at 4°C for 24 h, and then cul...

Embodiment 3

[0027] Embodiment 3: the influence of culture medium on the germination of Rhizoctonia chlamydospores

[0028](1) Transfer the test strain stored on the filter paper to a PDA medium plate, culture it at 28°C for 5 days, then transfer it to a new PDA medium plate, and cultivate it at 28°C for 10 days. Bacterial water Brush the chlamydospores of Rhizoctonia rubrum on the surface of the potato glucose medium, filter, and prepare 1 × 10 5 spores / mL of chlamydospore suspension, set aside.

[0029] (2) Culture medium preparation:

[0030] Water agar medium (WA medium): take 1000 mL of distilled water, add 16 g of agar powder, heat until the agar powder is completely dissolved, add water to make up to 1000 mL, and sterilize under high pressure at 121 °C for 25 min.

[0031] Agaricus bisporus decoction medium (MuDA medium): Slice 200 g of Agaricus bisporus, add 1000 mL of water to boil for 30 minutes, filter with double gauze, add 16 g of agar powder to the supernatant, heat until t...

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Abstract

The invention provides a chlamydospore germination medium of rubrum chlamydospore and a germination method, wherein each liter of the chlamydospore germination medium of rubrum chlamydospore contains the following raw material components: glucose 20 g, alanine 10 g, Agaricus bisporus 200 g, Agar powder 16 g. The suspension of chlamydospores of Rhizoctonia rubrum was cultured in the sporulation medium in the dark at 4°C for 24 hours to break dormancy, and then cultured in the dark at 25°C for 24 hours, and the germination rate reached 27.55%. The medium and germination method can be used to screen the fungicides for the germination of the chlamydospores of Rhizoctonia bisporus, which will play a positive role in the prevention and control of Agaricus bisporus.

Description

technical field [0001] The present invention relates to the technical field of plant pathogenic fungi research, in particular to a kind of Rhizoctonia rubra ( Mycogone rosea ) Chlamydospore germination medium and germination method. Background technique [0002] by fungi of the genus Verrucospora ( Mycogone LK. ex Chev.) infection of Agaricus bisporus brown rot is a worldwide soil-borne fungal disease. In 1888, Paris, France reported for the first time the outbreak of Agaricus bisporus brown rot. Since then, it has occurred to varying degrees in the United Kingdom, the United States, the Netherlands, Australia and other countries, causing huge economic losses. The disease has occurred in Agaricus bisporus planting areas in other places and is becoming more and more serious. The loss rate of some mushroom houses reaches 30%, and the serious mushroom house loss rate is as high as 50%-60%, or even no harvest, which seriously affects the output and production of Agaricus bis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C12N1/14C12R1/645
CPCC12N1/14C12N3/00
Inventor 石妞妞杜宜新陈福如阮宏椿杨秀娟甘林代玉立
Owner INST OF PLANT PROTECTION FAAS
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