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Method for optimizing ultrasonic-assisted extraction of tuber salep polysaccharide based on response surface methodology

A technology of ultrasonic-assisted extraction and response surface methodology, which is applied in the fields of polysaccharide/gum-containing food ingredients, applications, pharmaceutical formulations, etc., can solve the problems of reduced activity, loss of thermally unstable components, difficult industrial production, etc., and achieves polysaccharide yield. High, reduced extraction time, high antioxidant activity effect

Active Publication Date: 2019-11-29
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, infrared and microwave-assisted extraction requires high temperature, which will lead to the loss of some thermally unstable components and reduced activity at high temperature; the cost of supercritical fluid extraction is high, and it is difficult to industrialize production

Method used

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  • Method for optimizing ultrasonic-assisted extraction of tuber salep polysaccharide based on response surface methodology
  • Method for optimizing ultrasonic-assisted extraction of tuber salep polysaccharide based on response surface methodology
  • Method for optimizing ultrasonic-assisted extraction of tuber salep polysaccharide based on response surface methodology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 (response surface method)

[0024] a. Use a pulverizer to pulverize the dry products of Zhongyabai and tubers, pass through a 40-mesh sieve, and store in a ziplock bag at a temperature of 4°C;

[0025] b. Degrease the medium white and coarse powder obtained in step a with petroleum ether Soxhlet reflux for 20 hours at a temperature of 60° C., filter with suction, and evaporate the solvent from the filter residue under flowing air;

[0026] c. Soxhlet reflux with ethanol for 20 hours to remove the pigment and small molecules obtained in step b, and remove the pigment and small molecules, and filter the filter residue under flowing air to volatilize the solvent;

[0027] d. Using Design Expert 8.0.6 software to conduct Box-Behnken design BBD experiments with 3 factors and 3 levels, taking polysaccharide yield as the response value, ultrasonic power 390W, extraction temperature 60°C, total extraction time 50min, 4s off, 4s on Obtain extract under the conditio...

Embodiment 2

[0040] Embodiment 2 (comparison)

[0041] Hot water extraction:

[0042] a. Pulverize the dry products of Zhongyabai and tubers with a pulverizer, pass through a 40-mesh sieve, and store in a ziplock bag at a temperature of 4°C;

[0043] b. Degrease the medium white and coarse powder obtained in step a with petroleum ether Soxhlet reflux for 20 hours at a temperature of 60° C., filter with suction, and evaporate the solvent from the filter residue under flowing air;

[0044] c. Soxhlet reflux with ethanol for 20 hours to remove the pigment and small molecules obtained in step b, and remove the pigment and small molecules, and filter the filter residue under flowing air to volatilize the solvent;

[0045] d. Use a magnetic stirrer to extract the sample obtained in step c for 180 min at an extraction temperature of 100°C and an extraction liquid-to-solid ratio of 40 mg / mL, and use formula 1 to calculate the yield of the extraction liquid;

[0046] e. Centrifuge the Zhong Ya Ba...

Embodiment 3

[0048] The polysaccharide that embodiment 1 and embodiment 2 obtains carries out polysaccharide content and uses phenol-sulfuric acid colorimetric method to measure, and uronic acid content uses m-hydroxybiphenyl colorimetric method to measure, and protein content uses BCA method to measure, and its result is as table 3:

[0049] Table 3 Comparison of ultrasound-assisted extraction optimized by response surface methodology and traditional hot water extraction

[0050]

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Abstract

The invention relates to a method for optimizing the ultrasonic-assisted extraction of tuber salep polysaccharide based on response surface methodology. The method includes: crushing tuber salep, thenconducting sieving to separate impurities, performing degreasing with petroleum ether, conducting depigmentation with ethanol, carrying out natural air drying, then using water as a solvent, utilizing Design Expert 8.0.6 software for Box-Behnken design, taking the polysaccharide yield as the response value, adopting three-factor and three-level response surface analysis process to optimize the ultrasonic-assisted extraction of tuber salep polysaccharide, then centrifuging and concentrating the extracted liquid, performing precipitation with anhydrous ethanol, then conducting centrifugation, and subjecting the polysaccharide precipitate to vacuum freeze drying, thus obtaining the tuber salep polysaccharide. Compared with the traditional method, the method provided by the invention has obviously shortened time, significantly lowered temperature and higher antioxidation activity, is easy for scale-up production, and is one of the ideal methods for preparation of tuber salep polysaccharide. The study can provide reference for application and development of tuber salep polysaccharide in anti-oxidation and anti-aging functional food.

Description

technical field [0001] The invention relates to a method for optimizing the ultrasonic-assisted extraction of Rhizoma chinensis and polysaccharides based on the response surface method. The Rhizoma Rhizoma and polysaccharides obtained by the method can be used as raw materials for the preparation of medicines or health products related to the treatment or prevention of antioxidant diseases. Background technique [0002] Central Asian white and (TUBER SALEP), also known as European white and the dry tuber of Orchis chusua D.Don. Functions and indications: generating dampness and heat, strengthening yang and producing essence, nourishing the heart and producing blood, etc. Central Asian white and domestic distribution in Jilin, Heilongjiang, Sichuan, foreign distribution in the former Soviet Union, Japan and so on. Central Asia white and is a good source of glucomannan (16-55%). [0003] Polysaccharides, also known as polysaccharides, are polymeric sugar polymer carbohydrate...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P39/06A23L33/125
CPCA23V2002/00A61K31/715A23L33/125A61P39/06C08B37/0003A23V2200/30A23V2250/51
Inventor 阿布力米提·伊力热合巴提·努尔夏提阿吉艾克拜尔·艾萨
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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