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Amphipathic nucleobase functionalized cellulose polymer, micelle and preparation method thereof

A technology of cellulose polymer and nucleobase function, which is applied in the field of polymer material preparation, can solve the problems of high synthesis cost, wide application and limitation, and achieve the effect of high-value utilization

Active Publication Date: 2019-12-03
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high synthesis cost of nucleic acid materials limits its wide application to a large extent. At present, the synthesis of nucleic acid still relies on precise solid-phase synthesis methods, which limits the synthesis and application of most nucleic acid materials to milligram levels.

Method used

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  • Amphipathic nucleobase functionalized cellulose polymer, micelle and preparation method thereof
  • Amphipathic nucleobase functionalized cellulose polymer, micelle and preparation method thereof
  • Amphipathic nucleobase functionalized cellulose polymer, micelle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] Preparation method of amphiphilic nucleobase functionalized cellulose polymer micelles

[0123] (1) Preparation of cellulose macromolecular initiator

[0124] (1) Weigh 25g of 1-allyl-3-methylimidazolium chloride salt and 1g of microcrystalline cellulose, respectively, and dry them under vacuum at 80°C for 24h;

[0125] (2) Add microcrystalline cellulose to 1-allyl-3-methylimidazolium chloride salt, pump with water at 80°C for 2 hours, and then pump with oil for 1 hour;

[0126] (3) Slowly add 16mL of N,N-dimethylformamide to the mixture in step (2), react for 10min, and take an ice-water bath;

[0127] (4) Add 5 mL of 2-bromoisobutyryl bromide to the mixture of step (3), react at room temperature for 18 hours, purify the reaction product with methanol, precipitate the product in methanol, and dry to obtain a cellulose macromolecule Initiator.

[0128] (2) Preparation method of adenine acrylate monomer

[0129] (1) Weigh 13.5g of adenine, 0.811g of 2,6-di-tert-butyl...

Embodiment 2

[0142] Preparation method of amphiphilic nucleobase functionalized cellulose polymer micelles

[0143] (1) Preparation of cellulose macromolecular initiator

[0144] (1) Weigh 48g of 1-allyl-3-methylimidazolium chloride salt and 2g of microcrystalline cellulose, respectively, and vacuum-dry them at 80°C for 24h;

[0145] (2) Add microcrystalline cellulose to 1-allyl-3-methylimidazolium chloride salt, pump with water at 80°C for 2 hours, and then pump with oil for 1 hour;

[0146] (3) Add 30mL of N,N-dimethylformamide to the mixture in step (2), react for 10min, and take an ice-water bath;

[0147] (4) Add 10mL of 2-bromoisobutyryl bromide to the mixture of step (3), react at room temperature for 18h, purify the reaction product with methanol, precipitate the reaction product in methanol, and dry to obtain cellulose large molecular initiator.

[0148] (2) Preparation method of thymine acrylate monomer

[0149] Weigh 12.6g of thymine, 0.811g of 2,6-di-tert-butyl-4-methylphen...

Embodiment 3

[0164] Preparation method of amphiphilic nucleobase functionalized cellulose polymer micelles

[0165] (1) Preparation of cellulose macromolecular initiator

[0166] (1) Weigh 10 g of 1-allyl-3-methylimidazolium chloride salt and 0.5 g of microcrystalline cellulose, respectively, and vacuum-dry them at 80° C. for 24 hours;

[0167] (2) Add microcrystalline cellulose to 1-allyl-3-methylimidazolium chloride salt, pump with water at 80°C for 2 hours, and then pump with oil for 1 hour;

[0168] (3) Add 8 mL of N,N-dimethylformamide to the mixture in step (2), react for 10 min, and bathe in ice water;

[0169] (4) Add 2mL of 2-bromoisobutyryl bromide to the mixture of step (3), react at room temperature for 18h, purify the reaction product with methanol, precipitate the reaction product in methanol, and dry to obtain cellulose large molecular initiator.

[0170] (2) Preparation of guanine acrylate monomer

[0171] Weigh 15.1g of guanine, 0.811g of 2,6-di-tert-butyl-4-methylphen...

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Abstract

The invention discloses an amphipathic nucleobase functionalized cellulose polymer, a micelle and a preparation method thereof, and relates to the technical field of preparation of a polymer material.The polymer has a structure as shown in a formula (I), wherein Nu is (as shown in description), x, y and n are integers, x is more than or equal to 10 and less than or equal to 100, y is more than orequal to 20 and less than or equal to 200, n is more than or equal to 200 and less than or equal to 1200, and R is (as shown in description) or H or a polymer chain. The amphipathic nucleobase functionalized cellulose polymer has the following beneficial effects: a grafted copolymer is formed by polymerizing biocompatible cellulose, a nucleobase monomer or an N,N-dimethylacrylamide monomer; and the amphipathic nucleobase functionalized cellulose polymer micelle has high storage stability and has high biocompatibility and biodegradability.

Description

technical field [0001] The invention relates to the technical field of polymer material preparation, in particular to an amphiphilic nucleobase functionalized cellulose polymer, micelles and a preparation method thereof. Background technique [0002] Nucleic acid including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) is considered as a novel polymer functional material. Utilizing the specific hydrogen bond interactions between DNA strands, it is possible to realize the facile construction of beautifully ordered nanomaterials, such as DNA origami. Uniform nanoparticles can be spontaneously formed during the annealing process by utilizing long DNA strands to interact with a pre-designed number of short complementary strand-specific hydrogen bonds. Another way to construct nucleic acid nanoparticles is through the self-assembly of amphiphilic nucleic acid polymers, using nucleic acids as hydrophilic segments and covalently linking a segment of hydrophobic polymers. ...

Claims

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Application Information

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IPC IPC(8): C08F251/02C08F222/22C08F220/54C08F2/38C08J3/05C08L51/02
CPCC08F2/38C08F251/02C08F2438/01C08J3/05C08J2351/02C08F220/54
Inventor 华赞汪钟凯高陈闫阳阳李建军
Owner ANHUI AGRICULTURAL UNIVERSITY
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