Bacillus capable of inducing system resistance from tomato rhizosphere and screening method and application thereof

The technology of a bacillus and a screening method, applied in the field of microorganisms, can solve the problems of less tomato verticillium wilt and the like, and achieve the effects of good growth-promoting characters and good field growth-promoting functions.

Pending Publication Date: 2019-12-03
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few cases of plant rhizosphere growth-promoting bacteria controlling tomato Verticillium wilt

Method used

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  • Bacillus capable of inducing system resistance from tomato rhizosphere and screening method and application thereof
  • Bacillus capable of inducing system resistance from tomato rhizosphere and screening method and application thereof
  • Bacillus capable of inducing system resistance from tomato rhizosphere and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Screening and identification of Bacillus velezensis P1 strain.

[0032] The NA medium used in this example is beef extract peptone medium: beef extract 3.0g, peptone 10.0g, NaCl 5.0g, agar 20g, distilled water to 1000mL, adjust pH to 7.4-7.6, liquid medium without adding agar.

[0033] 1) Screening of Bacillus velezensis P1:

[0034] Collect the tomato rhizosphere soil in the pot experiment of Northeast Agricultural University Horticultural Station in Harbin City, Heilongjiang Province, which simulates continuous cropping in the field. The root surface soil is collected by shaking the root method, and the soil suspension is prepared according to the soil and water ratio of 1g:9mL. serial dilution to obtain 10 -3 Diluted concentration (i.e. dilute the suspension 100 times) of the soil suspension. Afterwards, smear 200 μl of soil suspension on 20ml of beef extract peptone agar medium (NA medium), repeat 3 plates, put them in a 28°C incubator and cultivate th...

Embodiment 2

[0043] Example 2. Determination of the growth-promoting ability and antagonistic potential of Bacillus velezensis P1 in the laboratory.

[0044] 1) Determination of IAA yield

[0045] The IAA-producing ability of the strain P1 screened in Example 1 was determined by the Salkowski colorimetric method. In autoclaved 20mL tryptone soybean broth liquid medium (TSB: tryptone 1.5%, soybean peptone 0.5%, sodium chloride 0.5%, dilute to 1000mL with distilled water, adjust the pH to 7.2±0.2, (for use after pressure steam sterilization), filter-sterilized tryptophan solution (final concentration of 100 mg / mL) was added, and the bacterial strain to be tested was inoculated.

[0046] At 28°C, cultured on a shaker for 48h. Make the fermentation broth at 8000r.min -1 Centrifuge for 10 min. Take 2 mL of supernatant, add 50 μL of 83% (10 mM / L) orthophosphoric acid, add 4 mL of Sackowski’s chromogenic reagent, mix well, and develop color at 25 ° C for 30 min in the dark. Absorbance value ...

Embodiment 3

[0059] Example 3. Application of Bacillus velezensis P1 strain for growth promotion and antagonistic functions in the field.

[0060] 1) Seed seedling cultivation and treatment

[0061] Disinfect the tomato seeds to be planted with Nongda 708 and sow them in the tomato continuous cropping soil, plant under the field conditions under the normal growth state, and carry out the following treatments (Table 2). Represents tomatoes inoculated with Verticillium dahliae, P1 represents tomatoes inoculated with Bacillus velezensis P1 strain, and "P1&Verticillium" represents tomatoes inoculated with both Bacillus velezensis P1 strain and Verticillium dahliae.

[0062] Table 2 Random block design

[0063]

[0064] 2) Treatment of Bacillus velezensis P1 and pathogenic bacteria

[0065] The PDA medium was autoclaved at 121°C for 15 minutes. Verticillium dahliae was inoculated into the medium. Incubate for 7 days at 28°C in the dark. Filter the culture through a double layer of gauze...

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Abstract

The invention discloses bacillus capable of inducing system resistance from tomato rhizosphere and a screening method and application thereof, and belongs to the field of microorganisms. In order to effectively relieve tomato continuous cropping obstacles and promote the growth of continuous cropping tomatoes, the invention provides Bacillus velezensis P1 which comes from tomato rhizosphere and can induce system resistance, and the strain preservation number of the Bacillus velezensis P1 is CCTCC No: M 2019456. The strain is obtained by collecting rhizosphere soil of continuous cropping tomatoes, carrying out continuous concentration gradient dilution, carrying out isolated culture on microorganisms in a soil suspension by adopting a plate coating method, and performing screening through aplate confrontation experiment. The strain has good growth-promoting traits and a good field growth-promoting function, and can inhibit the occurrence of verticillium wilt; and when the tomatoes areinfected by verticillium wilt pathogenic bacteria, the strain can induce tomato system resistance, and enhance the verticillium wilt resistance of the tomatoes. The strain has a good utilization prospect in the aspect of relieving tomato continuous cropping obstacles.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a bacillus from tomato rhizosphere capable of inducing systemic resistance, a screening method and application thereof. Background technique [0002] Tomato Verticillium wilt is an important disease in tomato production. It mostly occurs in the middle and late stages of tomato growth, causing the vascular bundles of the plant to become shallow and brown, and the leaves and plants gradually die from top to bottom, hindering the accumulation of nutrients in the tomato at the later stage. and transportation, seriously reducing the yield and quality of tomatoes. The pathogen of tomato verticillium wilt is Verticillium dahliae (Verticilliumdahliae), which is a fungus of the subphylum Idiomycetes. It has a wide range of hosts, is easy to spread, and can survive in the soil for a long time. In recent years, due to long-term continuous cropping cultivation, tomato verticillium ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/02C12N1/20C12N1/205C12R2001/07
Inventor 周新刚张建慧贾惠婷史亚晶陈少灿石记博吴凤芝
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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