Microsphere preparation loaded with transfer factors, preparation method of microsphere preparation, and application of microsphere preparation to aquaculture
A technology of transfer factor and aquaculture, which is applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, and veterinary vaccines, etc. It can solve the problems of lack of popularity in the field of aquaculture, uneconomical injections, and Fish stress and secondary infection and other problems, achieve the effect of improving fish body immunity, reducing fish body infection, and stabilizing activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] The invention provides a technical solution: a method for preparing a transfer factor-loaded microsphere preparation, comprising the following steps:
[0028] S1. Preparation of transfer factor: fresh spleens of healthy adult cows were used and sterilized to remove fascia and adipose tissue on the surface of the spleens, and then the spleens were mashed and homogenized to obtain a homogenate. Adjust the pH value of the homogenate to 3, and freeze and thaw repeatedly 5 times. The repeated freezing and thawing here refers to the cycle process of freezing and thawing the homogenate, which will cause multiple re-formation of ice crystals and damage the cell structure in the homogenate. Then the pH value was adjusted to 6.6, centrifuged at 3000r / min for 30min, and the supernatant was taken for hollow fiber filtration and 0.22μm microporous membrane filtration to obtain transfer factor extract.
[0029] S2. Screen transfer factor: The peptide and protein content in the transf...
Embodiment 2
[0032] The difference between this example and Example 1 mainly lies in: in step S1, the pH value of the homogenate was adjusted to 2, repeated freezing and thawing 3 times, then the pH value was adjusted to 6.5 and then centrifuged, and the supernatant was filtered to obtain the transfer factor extract. In step S2, the detected peptide and protein content was 6.32 mg / mL, the OD260 / OD280 value was 2.13, the protein assay was negative, and the transfer factor extract was qualified. In step S3, the qualified product is diluted to 1mg / mL with physiological saline, the mass volume concentration of the sodium alginate solution used is 1%, the oil phase is liquid paraffin containing 3% Span-80, the volume of the water phase and the oil phase The ratio is 1:1.5. The calcium chloride solution mass volume concentration that the present embodiment adopts is 6%, and the emulsified liquid mixes with calcium chloride solution and stirs 8min, and cleaning liquid adopts the sodium acetate s...
Embodiment 3
[0034] The difference between this example and Example 1 is that in step S1 of this example, the pH value of the homogenate was adjusted to 2.5, repeated freezing and thawing 6 times, and then the pH value was adjusted to 6.8, and the supernatant was filtered to obtain transfer. factor extract. In step S2, the detected peptide and protein content was 7.16 mg / mL, the OD260 / OD280 value was 2.24, the protein assay was negative, and the transfer factor extract was qualified. In step S3, the qualified product is diluted to 3mg / mL with physiological saline, the mass volume concentration of the sodium alginate solution used is 2%, the oil phase is liquid paraffin containing 5% Span-80, the volume of the water phase and the oil phase The ratio is 1:2.4. The calcium chloride solution mass volume concentration that present embodiment adopts is 10%, and the emulsified liquid mixes with calcium chloride solution and stirs 12min, and cleaning solution adopts the sodium acetate solution th...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More