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Method for improving drought tolerance of plants by improving photorespiration

A technology of photorespiration and drought tolerance, applied in botany equipment and methods, biochemical equipment and methods, plant peptides, etc., can solve the problem of drought tolerance reduction

Active Publication Date: 2019-12-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, our study found that plants overexpressing GDH and MS genes in chloroplasts, while inhibiting the expression of PLGG1 gene, had significantly reduced drought tolerance compared with the control

Method used

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  • Method for improving drought tolerance of plants by improving photorespiration
  • Method for improving drought tolerance of plants by improving photorespiration
  • Method for improving drought tolerance of plants by improving photorespiration

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1, the construction of carrier

[0039] GDH genes can be derived from prokaryotes or eukaryotes. The GDH genes provided by the present invention include but are not limited to the genes shown in Table 2. In order to construct the transformation vector, the GDH gene derived from Escherichia coli and the corresponding terminator sequence were artificially synthesized, including the chloroplast signal peptide, the GDH coding gene and the terminator. The nucleotide sequence is shown in SEQ ID NO.6 (the amino acid sequence is SEQ ID NO.6). ID NO. 7), BamHI and KpnI sites were set at the 5' and 3' ends, respectively.

[0040] MS genes can be derived from prokaryotes or eukaryotes. The MS genes provided by the present invention include but are not limited to the genes shown in Table 3. The MS gene of rice was artificially synthesized, including chloroplast signal peptide, MS coding gene and terminator, the nucleotide sequence is shown in SEQ ID NO. The ' ends are ...

Embodiment 2

[0047] Example 2, rice transformation

[0048] The method of obtaining transgenic rice is to use the existing technology (Lu Xiongbin, Gong Zuxun (1998) Life Science 10: 125-131; Liu Fan et al. (2003) Molecular Plant Breeding 1: 108-115). The mature and plump "Xiushui-134" seeds were selected and shelled to induce callus as the transformation material. Take the Agrobacterium strains constructed in Example 1 containing the plasmids pCambia1300-bar-GDH-MS-OsBASS-RNAi (GMOsBi) and pCambia1300-bar-GDH-MS-OsPGGL1-RNAi (GMOsPi) respectively. Pick a single colony to inoculate and prepare for transformation with Agrobacterium. The callus to be transformed is put into the Agrobacterium bacterium liquid with an OD of about 0.6 (the preparation of the Agrobacterium bacterium liquid: inoculate the Agrobacterium into the culture medium, and cultivate to an OD of about 0.6; the medium consists of 3 g / L K 2 HPO 4 , 1g / L NaH 2 PO 4 , 1g / L NH 4 Cl, 0.3g / L MgSO 4 ·7H 2 O, 0.15g / L KCl, 0...

Embodiment 3

[0049] Example 3. Soybean Transformation

[0050]The steps used here to obtain transgenic soybeans were derived from existing techniques (Deng et al., 1998, PlantPhysiology Communications 34:381-387; Ma et al., 2008, Scientia Agricultura Sinica 41:661-668; Zhou et al., 2001 , Journal of Northeast Agricultural University 32:313-319). Select healthy, plump and mature soybeans of "Tianlong No. 1", sterilize them with 80% ethanol for 2 minutes, rinse with sterile water, and place them in a desiccator filled with chlorine gas (produced by the reaction of 50ml NaClO and 2ml concentrated HCl) Sterilize for 4-6 hours. The sterilized soybeans were sown into B5 medium in an ultra-clean workbench, and cultivated at 25°C for 5 days, while the optical density was 90-150μmol photons / m 2 ·s level. When the cotyledons turn green and burst the seed coat, sterile sprouts will grow. The sprouts with the hypocotyls removed were cut in half in length so that both explants had cotyledons and ep...

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Abstract

The invention discloses a method for improving drought tolerance of plants by improving photorespiration, which comprises the following steps: inhibiting or knocking out bile acid sodium cotransportergenes in the plants, and simultaneously overexpressing glyoxylate dehydrogenase genes and malate synthase genes. The method creatively discovers that the expression of the BASS6 gene in the plant isinhibited; glycolic acid dehydrogenase genes and malic acid synthase genes are overexpressed in chloroplast, light respiration can be remarkably reduced, the photosynthetic efficiency of plants can beimproved, the biomass or yield of the plants can be increased, more importantly, the drought tolerance of the transgenic plants is remarkably higher than that of plants inhibiting PLGG1 gene expression, and the drought tolerance is improved by 5%-50%.

Description

[0001] (1) Technical field [0002] The invention relates to a method for improving the drought resistance of plants by improving photorespiration. The photosynthetic efficiency, biomass or yield of plants can be improved by means of transgenic methods, and plant planting resources can be improved. [0003] (2) Background technology [0004] The increase in the number of human beings and the improvement of living standards require more food and feed to be consumed, which requires more food to be harvested on limited land. Therefore, it is very important to breed new high-yielding plant varieties. [0005] The photosynthesis products of the whole plant are all derived from the enzyme catalyzed CO 2 converted to organic carbon compounds. Ribulose 1,5-diphosphate carboxylase / oxygenase (RubisCO) is a carboxylase in the Calvin-Benson (CB) cycle. As RubisCO and CO 2 or O 2 can react, RubisCO and O 2 The reaction produces phosphoglycolic acid, which enters the photorespiration c...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/53C12N15/54A01H5/00A01H6/46A01H6/54
CPCC12N15/8273C12N15/8218C07K14/415C12N9/1025C12N9/0006C12Y203/03009C12Y101/99014
Inventor 王东芳张先文沈志成
Owner ZHEJIANG UNIV
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