Antimicrobial polymers and antimicrobial hydrogels
A hydrogel, polymer technology, used in antibacterial drugs, topical antibacterial agents, botanical devices and methods, etc.
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Embodiment 1
[0151] In this example, a method with Durable antimicrobial hydrogel coatings for contact lenses. The thus developed PEI-decane-PEGMA hydrogel coating exhibited excellent broad-spectrum antibacterial activity against both Gram-negative and Gram-positive bacteria and fungi. The % kill and log reduction of the coating were above 99.99% and 4.0, respectively.
[0152] Materials and methods
[0153] Material
[0154] Branched polyethyleneimine (PEI, Sigma-Aldrich, M n=10 4 , M w / M n =2.5) freeze-dried to dryness. Chlorine-functionalized polyethylene glycol methacrylate (Cl-PEGMA) was synthesized as briefly described below. First, 11.8 mL (35.3 mmol) of PEGMA was dissolved in 100 mL of toluene, 11.25 mL (141.2 mmol) of chloroacetyl chloride was added to the solution, and then stirred and refluxed at 120° C. for 24 hours. After the reaction, the solution was subjected to solvent evaporation, followed by adding dichloromethane (100 mL) to the concentrate for dissolution. P...
Embodiment 2
[0210] In this example, polyethyleneimine-grafted-polyethylene glycol methacrylate (PEI-PEGMA) and its alkylated form with the addition of decane groups (PEI-decane- PEGMA) in combination with UV photopolymerization to develop a durable antimicrobial hydrogel coating for biomedical devices. These PEI-PEGMA and PEI-decane-PEGMA coatings showed excellent broad-spectrum antibacterial activity against both Gram-negative and Gram-positive bacteria and fungi. The % kill and log reduction of the hydrogel were higher than 99.99% and 7.0, respectively. The hydrogel is biocompatible with human cells as it was shown to have over 95% cell viability when tested in vitro against human dermal fibroblast (HDF) cells.
[0211] Materials and methods
[0212] Material
[0213] Branched polyethyleneimine (PEI, Sigma-Aldrich, M n =10 4 , M w / M n =2.5) freeze-dried to dryness. 1-bromodecane, sodium hydroxide, potassium carbonate, isopropanol, polyethylene glycol methacrylate (PEGMA, M n =...
Embodiment 3
[0323] In this example, a polymer was developed by UV photopolymerization of polyethyleneimine-graft-polyethylene glycol methacrylate (PEI-PEGMA) followed by sonication in ethanol and water for 2 h to remove residual monomers. Non-leachable cationic polymer-based hydrogels for wound infection control. The UV-visible absorbance test supported the conclusion of non-leaching, as no signal was detected in the solution (100 mg of hydrogel in 5 mL of water) where the hydrogel was soaked for 24 h, while even at a low concentration of 1 μg / mL of hydrogel polymer A peak can also be observed at 200 nm in .
[0324] In a biofilm infection model comparing the efficacy of hydrogels with commercially available antimicrobial wound dressings (Allevyn Ag and AlgisiteAg, Smith & Nephew), PEI hydrogels performed better, killing 4 logs (over 99.99%) of resistant Staphylococcus aureus (MRSA USA300), while commercial antimicrobial dressings only reduced bacterial counts by one log (over 90%). Fur...
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