Regeneration method of Camellia oleifera regeneration system
A system and technology of Camellia oleifera, applied in the directions of plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of low callus induction rate, low differentiation rate, can not meet the requirements of factory seedling raising, etc., to improve the induction rate and Differentiation rate, the effect of improving the differentiation rate
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[0031] Example 1
[0032] This embodiment provides a regeneration method of Camellia oleifera leaves, such as figure 1 Shown, including:
[0033] (1) Disinfection of explants
[0034] Pick the young leaves of ‘Changlin No. 4’ ordinary camellia, soak them in detergent for 5-10 minutes, then rinse them with tap water for 1-2 hours, and absorb the water with absorbent paper. On the ultra-clean workbench, soak the leaves with 70%-75% alcohol for 10-30s, clean them with sterile water 3-4 times, then disinfect the surface with 0.08-0.12% HgCl2 for 8-12min, then use sterile water Wash 6-8 times.
[0035] (2) Callus induction culture
[0036] The specific cultivation includes: putting the leaves on sterile filter paper, using a scalpel to draw 2-4 lines perpendicular to the main vein of the leaves, and then peeling the leaves with tweezers to inoculate the callus induction medium. Inoculate 3-8 leaves per dish (90mm). Place the inoculated petri dish in a culture room for dark culture at a ...
Example Embodiment
[0049] Example 2
[0050] This embodiment provides a method for regeneration of Camellia oleifera leaves, including:
[0051] (1) Callus induction culture
[0052] The leaf material is the leaf of the aseptic tissue culture seedling of "Changlin No. 4" common Camellia oleifera, and the specific culture is as in Example 1.
[0053] The callus induction medium is: 1 / 2MS minimal medium (powder), 1.0mg / L 2,4-D, 0.5mg / L TDZ, 50g / L sucrose, 8g / L agar, 250mg / L Hydrolyzed casein, 250mg / L of hydrolyzed milk protein, 100mg / L of proline, 100mg / L of glycine, 100mg / L of glutamine, 100mL / L of coconut water, and 1g / L of PPVP and 10mg / L silver nitrate, and adjust the pH to 5.6; the callus induction rate is 100%.
[0054] (2) Callus differentiation culture
[0055] The specific cultivation is as in Example 1.
[0056] The differentiation medium is: 1 / 2MS medium (powder), 0.05mg / L NAA, 1.0mg / L 6-BA, 0.05mg / L TDZ, 30g / L sucrose, 7g / L agar , 300mg / L hydrolyzed casein, 300mg / L hydrolyzed milk protein, 150...
Example Embodiment
[0061] Example 3
[0062] This embodiment provides a method for regeneration of Camellia oleifera leaves, including:
[0063] (1) Callus induction culture
[0064] The leaf material is the leaf of the aseptic tissue culture seedling of "Changlin No. 4" common Camellia oleifera, and the specific culture is as in Example 1.
[0065] The callus induction medium is: 1 / 2MS minimal medium (powder), 1.0mg / L 2,4-D, 1.0mg / L TDZ, 70g / L sucrose, 8g / L agar, 500mg / L Hydrolyzed casein, 500mg / L of hydrolyzed milk protein, 150mg / L of proline, 150mg / L of glycine, 150mg / L of glutamine, 100mL / L of coconut water, and 2g / L of PPVP and 10mg / L silver nitrate, and adjust the pH to 5.8; the callus induction rate is 100%.
[0066] (2) Callus differentiation culture
[0067] The specific cultivation is as in Example 1.
[0068] The differentiation medium is: 1 / 2MS medium (powder), 0.05 mg / L NAA, 2.0 mg / L 6-BA, 0.01 mg / L TDZ, 300 g / L sucrose, 7 g / L agar , 500mg / L hydrolyzed casein, 500mg / L hydrolyzed milk protei...
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