Method for separating triterpene alcohols and sterols from rice bran crude sterols

A technology of triterpene alcohols and sterols, which is applied in the field of rice bran crude sterols, can solve the problems of rice bran crude sterol resources such as idleness, discarding, and environmental pollution, and achieve the effects of realizing waste recycling, solving solid waste treatment problems, and considerable economic benefits

Active Publication Date: 2020-12-22
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the survey, the source of crude rice bran sterols is very rich. A rice bran oil plant with an annual output of 100,000 tons will produce about 500 tons of waste rice bran crude sterols a year. Due to technical limitations, the utilization of rice bran crude sterols has not yet been realized. , causing the precious rice bran crude sterol resources to be idle or discarded as solid waste, resulting in environmental pollution and huge waste of resources

Method used

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  • Method for separating triterpene alcohols and sterols from rice bran crude sterols
  • Method for separating triterpene alcohols and sterols from rice bran crude sterols
  • Method for separating triterpene alcohols and sterols from rice bran crude sterols

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Effect test

Embodiment 1

[0041] Embodiment 1: a kind of method for separating sterol and triterpene alcohol from rice bran crude sterol, comprises the following steps:

[0042] 1) Saponification: Mix 1g of rice bran crude sterol with 100ml of ethanol and 1.4g of potassium hydroxide, ultrasonically decompose it at 40KHZ for 10min, put it into a flask and heat it to reflux for about 6h under the protection of nitrogen to obtain a saponified solution.

[0043] 2) Extraction: Open the reflux device immediately after saponification, continue heating, and evaporate about half of the ethanol. After evaporating and concentrating, 100ml of water was added, and then extracted several times with diethyl ether, and the organic solvent layers were combined to obtain the extract.

[0044] 3) Neutralization and evaporation: adding 25% dilute hydrochloric acid to the extract to make the pH after neutralization 7, then washing with water to separate the liquid, evaporating and spin-drying to obtain unsaponifiable prod...

Embodiment 2

[0068] Embodiment 2: a kind of method for separating sterol and triterpene alcohol from rice bran crude sterol, comprises the following steps:

[0069] 1) Saponification: Mix 1 g of rice bran crude sterol with 100 ml of ethanol and 1.4 g of potassium hydroxide, ultrasonically decompose it at 40 KHz for 10 min, and leave it at room temperature for about 24 h to obtain a saponified solution.

[0070] 2) Extraction: After saponification, heat the saponified solution to evaporate about half of the ethanol. After evaporating and concentrating, 100 ml of water was added, followed by repeated extraction with ethyl acetate, and the ethyl acetate layers were combined to obtain an extract.

[0071] 3) Neutralization and evaporation: adding 25% dilute hydrochloric acid to the extract to make the pH after neutralization 7, then washing with water to separate the liquid, evaporating and spin-drying to obtain unsaponifiable product C.

[0072] 4) Column separation: the unsaponifiable matte...

Embodiment 3

[0078] Embodiment 3: a kind of method for separating sterol and triterpene alcohol from rice bran crude sterol, comprises the following steps:

[0079] 1) Saponification: Mix 5 g of rice bran crude sterol with 500 ml of ethanol and 9 g of potassium hydroxide, ultrasonically decompose it at 40 KHz for 30 min, and leave it at room temperature for about 24 h to obtain a saponified solution.

[0080] 2) Extraction: After saponification, heat the saponified solution to evaporate about half of the ethanol. After evaporating and concentrating, 300ml of water was added, and then extracted several times with ethyl acetate, and the ethyl acetate layers were combined to obtain an extract.

[0081] 3) Neutralization and evaporation: adding 25% dilute hydrochloric acid to the extract to make the pH after neutralization 7, then washing with water to separate the liquid, evaporating and spin-drying to obtain unsaponifiable product C.

[0082] 4) Column separation: the unsaponifiable matter ...

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Abstract

The method of being separated from the rough alcohol of the rice bran and the melol alcohol involves the coarse alcohol of the rice bran.1) Mixing the coarse alcohol and alcohol and alkali of rice bran to be mixed with alcohol and alkali, and the saponification liquid B is obtained after the saponification reaction;Extracting liquid; 3) Add the extracting solution to the acid, wash and rotate after neutralizing the pH value, and recycle the organic solvent layer to obtain no saponin C; 4) Step 3) Separate Silicon columns on the silicon column of no saponin C, useThe flow phase was washed away, the elution was collected, and through the thin layer of detection, the alcohol eluctation and triple glycolic liquid were combined, and the glycol and tripleterol were rotated.It can be directly separated from the thick alcohol alcohol of the waste rice bran to obtain the alcohol and tripleterol to achieve waste utilization and avoid pollution of the coarse and alcohol of rice bran to the environment.High income, product purity is as high as 99 %.

Description

technical field [0001] The invention relates to rice bran crude sterols, in particular to a method for separating triterpene alcohols and sterols from rice bran crude sterols. Background technique [0002] As people pay more and more attention to health, triterpenes and phytosterols play an increasingly important role in the fields of food, medicine, health care, feed addition, beauty and skin care. Triterpenes are a class of terpenoids whose basic core is composed of 30 carbon atoms. They have the functions of lowering cholesterol, moisturizing and skin care, protecting liver and liver, anti-tumor, regulating immunity, anti-inflammation, analgesia and sedation, anti-aging, anti-inflammatory oxidation etc. Sterol is a kind of sterol compound, which is widely distributed in nature. It has good anti-inflammatory and antioxidant effects, can inhibit the body’s absorption of cholesterol, prevent and treat coronary atherosclerotic heart disease, promote wound healing, and make m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00C07G99/00
CPCC07J9/00C07G99/00
Inventor 谢素原吴琳梓谢福发张前炎张美林
Owner XIAMEN UNIV
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