Deep fungal infection detection kit and detection method thereof

A technology for infection detection and deep fungus, applied in the field of biomedical diagnosis, can solve the problems of high false positive detection, false negative detection, low detection rate, etc., and achieve high sensitivity, high throughput, and easy operation
CN110628933AInactive Publication Date: 2019-12-31南京东万先卓生物科技研发有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
南京东万先卓生物科技研发有限公司
Publication Date
2019-12-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a fungal DNA extraction method and a two-step PCR amplification technology for detecting clinical fungal infections. The method comprises simple DNA extraction method,design of specific primers for candida albicans, cryptococcus neoformans, and aspergillus fumigatus, and establishment of a detection method for simultaneously detecting the above three fungi. The method is sensitive and specific, and has important significance for clinical diagnosis of three fungal infections.
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Description

technical field

[0001] The invention belongs to the field of biomedical diagnosis, and in particular relates to a deep fungal infection detection kit. Background technique

[0002] In recent years, due to the aging population, organ transplantation, tumor radiotherapy and chemotherapy, hematopoietic stem cell transplantation, the application of ultra-wide antibiotics and various catheter interventional treatments, the incidence of fungal infection has increased year by year. According to the site of infection, it can be divided into superficial infection and deep infection. Superficial infection includes skin, mucous membrane, nails, hair, subcutaneous tissue, etc., and deep infection includes lung, blood, gastrointestinal tract and other organ and tissue infections. The current effective treatment for fungal infections is still the use of antibiotics, so accurate and timely diagnosis of the disease is very important.

[0003] Currently, clinical methods for diagnosing fung...

Claims

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