Application of composition of natural plant extracts including rosemary extract, honeysuckle extract and ligusticum wallichii extract in cosmetics for sun protection, after-sun repair, inflammation resistance, photoaging resistance and whitening
A plant extract and after-sun repair technology, applied in the field of natural plant extract composition, can solve the problems of easy clogging of pores, skin damage, poor air permeability, etc., and achieve the effects of reducing inflammation, mild skin effect, and low production cost
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Embodiment 1
[0042] Example 1. Effects of rosemary extract, honeysuckle extract, and Chuanxiong extract on cell activity.
[0043] Immortalized human epidermal HaCaT cells were used at 1×10 5 The density of each well was inoculated into a 96-well plate. After the cells adhered to the wall, the original cell culture medium was replaced with 100 μL of 1640 medium containing drugs. Respectively (0mg / mL, 0.25mg / mL, 0.5mg / mL, 0.75mg / mL, 1mg / mL, 1.5mg / mL, 2mg / mL). After 24 hours, add CCK-8 to detect the proliferation activity of the cells. Add 10 μl of CCK-8 solution to each well, and incubate in a 37°C incubator for 1 hour, and the color of the medium turns orange; the specific incubation time depends on different cells, and the detection wavelength is the absorbance value at 450nm, the higher the absorbance value, the greater the proliferative activity of the cells, and vice versa. The result is as Picture 1-1 , 1-2, 1-3, compared with the control group without drugs, when the concentratio...
Embodiment 2
[0044] Example 2. Comparison of the protective effects of the composition, rosemary extract, and honeysuckle extract on UV-irradiated HaCaT cells.
[0045] Immortalized human epidermal HaCaT cells were used at 1×10 5 The density of each hole was inoculated into a 96-well plate. After the cells adhered to the wall, the original cell culture medium was replaced with 100 μL of 1640 medium containing drugs. Experimental groups: control group, ultraviolet group, composition group (containing 0.25mg / mL rosemary extract and 0.25mg / mL honeysuckle extract), rosemary extract group (0.25mg / mL, 0.5mg / mL), honeysuckle extract group (0.25mg / mL, 0.5mg / mL). After the drug was incubated for 24 hours, the drug medium was replaced with PBS and irradiated by a cell sunlight ultraviolet simulator (purchased from France) at a dose of 50 mJ / cm 2 UVB+550mJ / cm 2 UVA, in which the cells in the experimental control group were neither treated with drugs nor irradiated with UVA. After the end of ultra...
Embodiment 3
[0046] Example 3. Comparison of the protective effect of the composition, rosemary extract, and Chuanxiong extract on UV-irradiated HaCaT cells.
[0047] The implementation process refers to Example 2, experimental grouping: control group, ultraviolet group, composition group (containing 0.25mg / mL rosemary extract and 0.25mg / mL Chuanxiong extract), rosemary extract group (0.25mg / mL mL, 0.5mg / mL), Chuanxiong extract group (0.25mg / mL, 0.5mg / mL). The result statistics are as follows image 3 shown. The activity of HaCaT cells after ultraviolet irradiation was significantly reduced, and semi-lethal phenomenon appeared. After adding the composition, rosemary, and Chuanxiong extracts, the cell activity was enhanced. Compared with the UV control group, the difference was significant and statistically significant, * *p<0.01, ***p<0.001, compared with the rosemary extract group and Chuanxiong extract group, the cell viability increased significantly in the composition group, the diff...
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