Magnetic covalent organic framework material for glycopeptide enrichment and its preparation method and application
A covalent organic framework and magnetic technology, applied in the field of biomaterials, can solve the problems of unsatisfactory glycopeptide enrichment performance and lack of functional modification of hydrophilic performance, and achieve improved hydrophilic performance, good magnetic response performance, high magnetic The effect of saturation intensity
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Embodiment 1
[0037] Embodiment 1 hydrothermal method prepares superparamagnetic Fe 3 o 4 nano magnetic ball
[0038] The Fe used in the following examples 3 o 4 The specific preparation process of nano magnetic balls is as follows: the raw material 2.43g FeCl 3 ·6H 2 O, 3.6g NaAc (sodium acetate) and 0.4g Na 3 CT (sodium citrate) was added to a reaction kettle containing 60mL of ethylene glycol, and magnetically stirred for 1 hour to mix the above-mentioned raw materials evenly; then the stirring bar was removed, and the temperature of the reaction kettle was raised to 200°C for 12 hours of reaction; then the reaction kettle Cool to room temperature, magnetically separate the reaction solution and collect the isolated solid product; then wash the solid product with ethanol and deionized water successively (each washing solution is washed five times), to obtain Fe 3 o 4 nano magnetic balls.
[0039] The Fe obtained by this method 3 o 4 Nanomagnetic spheres can be uniformly dispers...
Embodiment 2- Embodiment 8
[0041] Embodiment 2-embodiment 8 prepares Fe 3 o 4 / COFs nanoparticles (220nm Fe 3 o 4 nano magnetic balls)
[0042] Take the raw material according to table 1, and prepare Fe according to the following operating process in conjunction with the process parameters given in table 1 3 o 4 / COFs nanoparticles:
[0043] Will Fe 3 o 4 Nanomagnetic spheres, 1,3,5-tris(4-aminophenyl)benzene (Tab) and 2,5-divinyl-1,4-benzenedicarbaldehyde (Dva) were added to the Methyl sulfoxide solvent in the Erlenmeyer flask, and then the two organic ligands were completely dissolved under ultrasonic conditions, and the Fe 3 o 4 The magnetic nanospheres are completely dispersed to obtain a uniformly dispersed mixed solution. Then, under ultrasonic conditions, acetic acid is added dropwise to the obtained mixed solution. After the addition is completed, the entire reaction system is incubated at room temperature. Finally, the resulting reaction solution is magnetically separated and the isol...
Embodiment 9- Embodiment 16
[0047] Embodiment 9-embodiment 16 prepares Fe 3 o 4 / COF-GSH material (use 270nm Fe 3 o 4 / COFs nanoparticles)
[0048] The following Examples 9-16 adopted the mercaptoene click reaction method, and the reaction device used was a two-neck flask reaction vessel heated by an oil bath and magnetically stirred.
[0049] Take the raw material according to table 2, and prepare Fe according to the following operating process in conjunction with the process parameters given in table 2 3 o 4 / COF-GSH material:
[0050] Azobisisobutyronitrile, glutathione and Fe 3 o 4 / COFs nanoparticles were sequentially added to 20mL composite solvent (the composite was obtained by mixing ethanol and deionized water according to the volume ratio of 1:3), and ultrasonic treatment was used to make Fe 3 o 4 / COFs nanoparticles are completely dispersed, and azobisisobutyronitrile and glutathione are completely dissolved. Then, nitrogen purging was used to deoxygenate the mixed liquid for 1 h; th...
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