Amino acid nano hydrogel as well as preparation method and application thereof

A nano-hydrogel and amino acid technology, applied in the preparation method of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of multiple action sites, structural denaturation, uneven imprinted holes, etc., and achieve the preparation method The effect of simplicity and mild reaction conditions

Active Publication Date: 2020-01-14
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned polymers with imprinted holes prepared by molecular imprinting technology must use template proteins, cloned proteins or specific peptides as template molecules in the preparation process, and these expensive template protein molecules with unstable physical and chemical properties During the imprinting process, the structure will be denatured due to the elution of acidic and polar solvents, making it impossible to reuse; and the template elution often has incomplete elution, which will lead to uneven imprinted holes and affect the polymer. Absorptivity, even leakage occurs when the polymer adsorbs the target protein, resulting in inaccurate adsorption and other problems
In addition, the protein itself has a complex structure and many action sites, and the three-dimensional network structure of the prepared polymer is unstable, which may easily cause the collapse of the recognition site.

Method used

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  • Amino acid nano hydrogel as well as preparation method and application thereof
  • Amino acid nano hydrogel as well as preparation method and application thereof
  • Amino acid nano hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Prepare polymer A, namely hydrophobic amino acid nanohydrogel, including phenylalanine nanohydrogel and leucine nanohydrogel.

[0077] 1. Preparation of functional monomer N-acryloyl-L-phenylalanine

[0078] 3.63g (0.020mol) L-phenylalanine (L-Phe) and 1.6g (0.04mol) sodium hydroxide are dissolved in 20mL of water, under 0℃, slowly add 1.75mL (0.022mol) propylene while stirring Acid chloride, drip in 0.5h. After the addition is complete, continue stirring at room temperature for 6 hours. Adjusted to pH2 with concentrated hydrochloric acid, filtered with suction, washed with water to neutral, dried in vacuum at 50°C for 24h to obtain a white powdery solid, N-acryloyl-L-phenylalanine (APA), the yield was 45.7% (2.74) g).

[0079] 2. Preparation of functional monomer N-acryloyl-L-leucine

[0080] 2.6238g (0.02mol) L-leucine and 1.6g (0.04mol) sodium hydroxide are dissolved in 30ml of water, under 0℃, slowly add 1.625ml (0.02mol) acryloyl chloride dropwise while stirring, drop w...

Embodiment 1-2

[0088] Prepare polymer A, namely hydrophobic amino acid nanohydrogel, including phenylalanine nanohydrogel and leucine nanohydrogel. The steps for preparing phenylalanine nanohydrogel in this embodiment are as follows:

[0089] N-acryloyl-L-phenylalanine (APA) (285mg, 40mol%), N-isopropylacrylamide (213.3mg, 58mol%), N,N'-methylenebisacrylamide (10mg, 2mol%), ammonium sulfate (30mg) was dissolved in 50ml water containing 10mg sodium lauryl sulfate.

[0090] Blow nitrogen for 20 minutes, and heat in an oil bath at 65°C for 3 hours. The obtained polymer solution was put into a dialysis bag (MW 12000 ~ 14000) for 4 days and the water was changed twice a day. The dialysate inside the dialysis membrane is collected and freeze-dried to obtain phenylalanine nanohydrogel (APA@NPs).

[0091] In the same way, leucine nanohydrogels (A-Leu@NPs) were prepared, but the functional monomer N-acryloyl-L-leucine (A-Leu, 240.7mg, 40mol%) replaced N-acryloyl -L-Phenylalanine (APA).

Embodiment 2

[0093] Prepare polymer B, that is, positively charged amino acid nanohydrogel, including arginine nanohydrogel and histidine nanohydrogel.

[0094] 1. Preparation of functional monomer N-acryloyl-arginine

[0095] 1.7420g (0.01mol) L-arginine and 2.12g (0.02mol) sodium carbonate are dissolved in 30ml water, placed below 0℃, slowly add 0.95ml (0.012mol) acryloyl chloride dropwise while stirring, drop within 0.5h Finish. After the addition is complete, continue stirring for 3.0 hours at room temperature. Adjust the pH to 2 with concentrated hydrochloric acid, wash with saturated sodium chloride solution, extract with ethyl acetate:isopropanol (1:1) 2×40ml, dry the organic layer over anhydrous magnesium sulfate, filter, and evaporate under reduced pressure Solvent. The residue was separated by silica gel column chromatography [V(chloroform):V(methanol)=1:1] to obtain N-acryloyl-arginine (A-Arg) as a yellow liquid with a yield of 15.00%.

[0096] 2. Preparation of functional monomer ...

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Abstract

The invention discloses an amino acid nano hydrogel as well as a preparation method and application thereof. The amino acid nano hydrogel has an amino acid side chain, has interactions similar to natural protein-natural protein with protein, has specific recognition capabilities for human serum protein and biomarkers such as beta-amyloid protein, brain natriuretic peptide and a gastrin release peptide precursor, and can be adopted to adsorb a target protein. The amino acid nano hydrogel is capable of improving the recognition capability of a polymer, is simple in preparation method, mild in reaction condition, has a temperature response property, and has the potential of being developed into an artificial simulated antibody or protein purification high polymer material with stable properties. The amino acid nano hydrogel is a polymer A or a polymer B, wherein the polymer A consists of segments of a formula (I), a formula (II) and a formula (III) shown in the description; and the polymer B consists of segments of a formula (IV), a formula (II), a formula (V) and a formula (III) shown in the description.

Description

Technical field [0001] The present invention relates to the technical field of non-biological antibodies, and more specifically, to an amino acid nano hydrogel and a preparation method and application thereof. Background technique [0002] Antibodies currently play an important role in clinical detection, disease diagnosis and treatment, and purification of antigens and proteins. Artificially prepared natural antibodies are mainly obtained through methods such as polyclonal antibodies, monoclonal antibodies, and genetically engineered antibodies. Among them, monoclonal antibodies are the most commonly used in clinical diagnostic testing, but the preparation cycle is long, the cost is high, and it is difficult to scale production, so the price is expensive , And cannot be recycled; on the other hand, antibodies, like proteins, are sensitive to the environment, have poor stability, and are easily inactivated under high temperature and harsh conditions, which in turn brings great in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/54C08F220/58C08F222/38C08F220/60C08J3/075C07K14/765C07K1/14C08L33/24
CPCC07K14/765C08F220/54C08F220/60C08J3/075C08J2333/24C08F220/58C08F222/385
Inventor 朱全红朱泳妍吴登宇
Owner SOUTHERN MEDICAL UNIVERSITY
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