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Ultrasonic-mediated silk fibroin composite collagen hydrogel and preparation method thereof

A technology of collagen hydrogel and silk fibroin, which is applied in the field of ultrasound-mediated silk fibroin composite collagen hydrogel and its preparation, can solve the problem of narrow adjustable range of structure and performance, troubles for researchers to innovate, and limited application range, etc. problem, to achieve the effect of efficient and quick preparation method, easy industrialization and popularization and application, and wide application prospect

Inactive Publication Date: 2020-01-17
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Of course, as a tissue engineering scaffold, although pure silk fibroin hydrogel has good cell affinity, it also has disadvantages such as poor flexibility, brittleness, and narrow adjustable range of structure and performance. range of applications
[0005] At present, there are few reports on ultrasound-mediated silk fibroin-collagen hydrogel, and the easy phase separation phenomenon after direct mixing of collagen and silk fibroin solutions is still a constraint for researchers to innovate

Method used

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  • Ultrasonic-mediated silk fibroin composite collagen hydrogel and preparation method thereof
  • Ultrasonic-mediated silk fibroin composite collagen hydrogel and preparation method thereof
  • Ultrasonic-mediated silk fibroin composite collagen hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of ultrasound-mediated silk fibroin composite collagen hydrogel

[0040] (1) Dissolve 0.45g of collagen completely in 1M 100mL acetic acid solution at 2-8°C, adjust the pH value to 7.0, and obtain a uniform collagen solution for use.

[0041] (2) Dissolve 10g degummed silk in CaCl 2 / C 2 h 5 OH / H 2 O (n=1:2:8) ternary solution, dissolved at 75±2°C for 4 hours, and then dialyzed in deionized water for 3 days with a dialysis bag with a molecular weight cut-off of 3500Da to obtain a concentration of 5% (w / The silk fibroin solution of v) is diluted to a concentration of 3% (w / v) with deionized water for subsequent use;

[0042] (3) Measure 50mL of the 5% silk fibroin solution prepared in step (2), use ultrasonic power of 1000 watts, ultrasonic power ratio of 40% and normal temperature to ultrasonically treat the silk fibroin solution for 1min, and then place it in 2-8 Stand-by at ℃;

[0043] (4) Measure 20mL of collagen solution and 10mL of silk...

Embodiment 2

[0045] Example 2 Preparation of ultrasound-mediated silk fibroin composite collagen hydrogel

[0046] (1) Dissolve 1 g of collagen completely in 0.3M 200mL acetic acid solution at 2-8°C, adjust the pH value to 5.0, and obtain a uniform collagen solution for use.

[0047] (2) 10g degummed silk was dissolved in 9.3M LiBr solution, dissolved at 60±5°C for 4h, and after the dissolution was complete, it was dialyzed in deionized water for 3 days with a molecular weight cut-off of 1000Da to obtain a concentration of 3% (w / v ) of the silk fibroin solution and adjust the pH to 5 for stand-by;

[0048] (3) Measure 30 mL of the 3% silk fibroin solution prepared in step (2), treat the silk fibroin solution for 10 seconds with an ultrasonic power of 500 watts, an ultrasonic power ratio of 60% and normal temperature, and then place it in 2-8 Stand-by at ℃;

[0049] (4) Measure 30mL collagen solution and 10mL silk fibroin solution in turn from (1) and (3) and mix them well to form a mixed...

Embodiment 3

[0050] Example 3 Ultrasound-mediated preparation of silk fibroin composite collagen hydrogel

[0051] (1) Dissolve 8g of collagen completely in 0.3M 200mL acetic acid solution at 2-8°C, adjust the pH value to 5.0, and obtain a uniform collagen solution for use.

[0052] (2) 15g degummed silk was dissolved in 40% CaCl 2 In the solution, dissolve at 98±2°C for 4 hours. After the dissolution is complete, use a dialysis bag with a molecular weight cut-off of 12,000 Da to dialyze in deionized water for 3 days to obtain a silk fibroin solution with a concentration of 8% (w / v) and adjust the pH to 5. stand-by;

[0053] (3) Measure 50 mL of the 8% silk fibroin solution prepared in step (2), process the silk fibroin solution for 30 seconds with an ultrasonic power of 500 watts, an ultrasonic power ratio of 60% and an ice bath for 30 seconds, and then place it in 2- Stand-by at 8°C;

[0054] (4) Measure 10mL of collagen solution and 10mL of silk fibroin solution from (1) and (3) in t...

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PUM

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Abstract

The invention discloses an ultrasonic-mediated silk fibroin composite collagen raw hydrogel and a preparation method thereof. A regenerated silk fibroin solution is treated in ultrasonic short time topromote conformation transformation and physical crosslinking, and then the regenerated silk fibroin solution is mixed with collagen solution according to a certain proportion and uniformly stirred,the solution pH value is regulated, and the incubation is performed under a certain temperature to generate composite hydrogel. The mass ratio of the silk fibroin to the collagen is 99: 1-1: 99. The ultrasonic-mediated fibroin composite collagen hydrogel prepared by the invention is safe, nontoxic, good in biocompatibility, short in gelation time, controllable in degradation time, excellent in mechanical property, simple in preparation process, energy-saving, environment-friendly, low in cost and easy to industrialize, popularize and apply, and is physically blended in the whole process.

Description

technical field [0001] The invention relates to the field of hydrogels, in particular to an ultrasonic-mediated silk fibroin composite collagen hydrogel and a preparation method thereof. Background technique [0002] Hydrogel is a gel with a three-dimensional network structure that is formed by polymer chains and retains water in the spaces between polymers. It can well simulate the water environment of the extracellular matrix, and provide a microenvironment similar to the natural extracellular matrix for cell adhesion, proliferation and differentiation. It is an ideal material for regeneration, repair and reconstruction of tissue damage, so it is widely used in biological Medical fields, such as medical dressings, cartilage tissue repair, cell transplantation, drug delivery and tissue engineering scaffolds, etc. However, the current hydrogel products for tissue repair still face many problems, especially the lack of mechanical properties and the mismatch between the degra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08J3/075A61L27/48A61L27/52
CPCA61L27/48A61L27/52C08J3/075C08J2389/00C08J2489/00C08L89/00
Inventor 肖芸龙仕和朱向东张兴栋
Owner SICHUAN UNIV
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