Halohydrin dehalogenase mutant and application thereof

A halohydrin dehalogenase and mutant technology, applied in the biological field, can solve the problems of excellent catalytic performance, inability to meet industrial production, and few industrial production.
CN110699345APending Publication Date: 2020-01-17YANCHENG INST OF TECH
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
YANCHENG INST OF TECH
Publication Date
2020-01-17

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Abstract

The invention first discloses a halohydrin dehalogenase mutant and application thereof. According to the invention, a database mining technology is used to mine potential halohydrin dehalogenase genes, and the halohydrin dehalogenase genes are induced to express in engineering bacteria to obtain a new halohydrin dehalogenase. Semi-rational design and modification of the enzyme is performed, and (R, S)-PGE is used as a model substrate to obtain two mutants with improved stereoselectivity, which are Q159L and N160Lrespectively. The enantioselectivity of the two mutants is increased approximatelyfrom 9.85 to 21.80 and 21.10respectively. The selectivity of the mutant N160L is opposite to selectivity of the original enzyme. The selectivity to various substrates of the two mutants is improved to a certain extent. In particular, the E value of the mutant N160L for the substrate P-methylphenyl glycidyl ether is increased to 25.77, which is 13.93 times greater than E value of the original HHDHHis.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a halohydrin dehalogenase mutant and application thereof. Background technique

[0002] As important intermediates in pharmaceutical and chemical industries, halohydrin compounds are widely used in organic synthesis and pharmaceutical research and development. However, most halides in nature have disadvantages such as poor degradation ability, high toxicity and potential carcinogenicity. It has become one of the main environmental pollutants, so people pay more and more attention to its degradation treatment in order to reduce the pollution of this kind of organic halides to the natural environment and the potential threat to human beings. The use of traditional physical and chemical methods not only has harsh reaction conditions, but also may cause secondary pollution, while the use of biodegradation methods has outstanding advantages such as no pollution to the environm...

Claims

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