Lactobacillus gasseri LG08 with uric acid degradation effect and application thereof

A kind of Lactobacillus gasseri, the technology of action, applied in the field of microorganisms, can solve the problem of affecting the mitigation effect, degrading inosinic acid reports, whether unreported strains can degrade uric acid and the like

Active Publication Date: 2020-02-04
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it should be pointed out that there are many precursors that can synthesize uric acid contained in food. For example, some umami flavor agents that improve flavor contain not only disodium 5'-guanylate, but also disodium 5'-inosinate. None of the above strains have been reported to degrade inosinic acid
In addition, uric acid is also continuously produced during the body's own metabolic process. Although the above-mentioned strains can efficiently metabolize part of the precursors of uric acid synthesis, it has not been reported whether the above-mentioned strains can degrade the produced uric acid, which directly affects their response to the reduction of uric acid excretion. The relieving effect of hyperuricemia, such as primary hyperuricemia caused by decreased glomerular filtration, increased renal tubular reabsorption, and decreased renal tubular secretion, and this type of hyperuricemia accounted for the primary About 90% of episodic hyperuricemia

Method used

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  • Lactobacillus gasseri LG08 with uric acid degradation effect and application thereof
  • Lactobacillus gasseri LG08 with uric acid degradation effect and application thereof
  • Lactobacillus gasseri LG08 with uric acid degradation effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening of bacterial strains with the effect of degrading uric acid

[0026] 1) Strain screening

[0027] Extract 5mL of the diluted solution from healthy old man's feces in Bama Town, Hechi City, Guangxi Zhuang Autonomous Region, China (the crowd had never taken antibiotics before collection, had no history of taking probiotics, and had no history of gastrointestinal diseases), and serially diluted 10 times to 10 -6 , 200 μL of each dilution concentration was spread on the selective MRS medium, anaerobically cultured at 37°C for 48-72h, and different single colonies on the plate were isolated and obtained. The above-mentioned different single colonies were picked with a sterilized inoculation loop, streaked and purified on the MRS solid medium, and cultured anaerobically at 37°C for 48 hours to obtain a total of 9 pure lactic acid bacteria strains, which were named L1-L9 respectively.

[0028] 2) Analysis of the tolerance of isolated strains to simulated g...

Embodiment 2

[0046] Embodiment two strain identification

[0047] According to the bacterial strain L8 that embodiment one obtains, identification method is as follows:

[0048] The obtained strain L8 was identified by 16s rDNA sequencing and physiological and biochemical identification. The 16s rDNA universal primer sequence 27F was: 5'-AGAGTTTGATCCTGGCTCAG-3' (Seq ID No: 1), and 1492R was: 5'-GGTTACCTTGTTACGACTT-3' (Seq ID No: 2).

[0049] The 16S rDNA gene sequence of strain L8 was amplified and sequenced, and the PCR amplification product was sent to Shanghai Sangon Bioengineering (Shanghai) Co., Ltd. for sequencing. The nucleotide sequence of 16S rDNA of strain L8 is shown in Seq ID No: 3 . After 16s rDNA gene comparison, the similarity rate with the Lactobacillus gasseri strain in Genebank was 100%. Combined with the physiological and biochemical identification results, the strain L8 was identified as a strain of Lactobacillus gasseri, named Lactobacillus gasseri LG08, and its phys...

Embodiment 3

[0054] Example Lactobacillus trigerii LG08 degrades nucleosides and nucleotide product analysis

[0055] This example provides a method for detecting metabolites after degradation of adenosine, guanosine, inosine, adenosine, guanylate, inosine and 5'-guanylate disodium by Lactobacillus gasseri LG08 , including the following steps:

[0056] Add 5mmol / L adenosine, guanosine, inosine, adenylic acid, guanylic acid, inosine and 5'-guanylic acid disodium respectively in the ammonium acetate buffer solution, and the dry weight concentration of bacteria is 0.4g / L add PBS buffer solution to wash the bacteria cells, mix culture at 37°C for 2 hours, and then use ultrasonic crushing. Centrifuge for 15 min, take the supernatant and measure the concentration of metabolites (hypoxanthine, guanine, xanthine, uric acid) by mass spectrometry.

[0057] The mass spectrometry conditions are as follows: triple quadrupole mass spectrometer (Agilent 1290 series), reversed-phase chromatographic col...

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Abstract

The invention relates to the field of microorganisms, and particularly relates to Lactobacillus gasseri LG08 with a uric acid degradation effect and application thereof. The Lactobacillus gasseri LG08is preserved in the China General Microbiological Culture Collection Center (CGMCC) in July 18, 2018, with the preservation number being CGMCC No. 16131, and is classified and named as Lactobacillusgasseri, with the preservation address being the Institute of Microbiology, Chinese Academy of Sciences, No.3, Academy No.1, Beichen West Road, Chaoyang District, Beijing. The Lactobacillus gasseri LG08 has an excellent nucleoside and nucleotide degradation capability, can decompose most precursors for uric acid synthesis, reduce the body absorption of precursors for uric acid synthesis and directly and efficiently decompose uric acid in intestinal tracts, and can further inhibit the generation of uric acid and relieve inflammation and other side reactions by reducing the serum endotoxin leveland inhibiting the xanthine oxidase activity.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Lactobacillus gasseri LG08 capable of degrading uric acid and an application thereof. Background technique [0002] In recent years, with the continuous improvement of people's material living standards, the public's intake of high-purine foods such as seafood, meat, and beer has continued to increase. This has not only improved the happiness of the tip of the tongue, but also brought many chronic metabolic diseases , such as hyperuricemia. According to statistics, there are about 120 million patients with hyperuricemia in my country, which is close to 10% of the total population. Among them, middle-aged and elderly men and postmenopausal women are high-risk groups. Under normal circumstances, uric acid in the human body is continuously produced and excreted, and is maintained within a certain concentration range. However, once this balance is broken, it is easy to cause the uri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P19/06C12R1/225
CPCA61K35/747A61P19/06C12R2001/225C12N1/205
Inventor 方曙光吴明科朱建国刘春
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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