Lactobacillus gasseri LG08 with uric acid degradation effect and application thereof
A kind of Lactobacillus gasseri, the technology of action, applied in the field of microorganisms, can solve the problem of affecting the mitigation effect, degrading inosinic acid reports, whether unreported strains can degrade uric acid and the like
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] Example 1 Screening of bacterial strains with the effect of degrading uric acid
[0026] 1) Strain screening
[0027] Extract 5mL of the diluted solution from healthy old man's feces in Bama Town, Hechi City, Guangxi Zhuang Autonomous Region, China (the crowd had never taken antibiotics before collection, had no history of taking probiotics, and had no history of gastrointestinal diseases), and serially diluted 10 times to 10 -6 , 200 μL of each dilution concentration was spread on the selective MRS medium, anaerobically cultured at 37°C for 48-72h, and different single colonies on the plate were isolated and obtained. The above-mentioned different single colonies were picked with a sterilized inoculation loop, streaked and purified on the MRS solid medium, and cultured anaerobically at 37°C for 48 hours to obtain a total of 9 pure lactic acid bacteria strains, which were named L1-L9 respectively.
[0028] 2) Analysis of the tolerance of isolated strains to simulated g...
Embodiment 2
[0046] Embodiment two strain identification
[0047] According to the bacterial strain L8 that embodiment one obtains, identification method is as follows:
[0048] The obtained strain L8 was identified by 16s rDNA sequencing and physiological and biochemical identification. The 16s rDNA universal primer sequence 27F was: 5'-AGAGTTTGATCCTGGCTCAG-3' (Seq ID No: 1), and 1492R was: 5'-GGTTACCTTGTTACGACTT-3' (Seq ID No: 2).
[0049] The 16S rDNA gene sequence of strain L8 was amplified and sequenced, and the PCR amplification product was sent to Shanghai Sangon Bioengineering (Shanghai) Co., Ltd. for sequencing. The nucleotide sequence of 16S rDNA of strain L8 is shown in Seq ID No: 3 . After 16s rDNA gene comparison, the similarity rate with the Lactobacillus gasseri strain in Genebank was 100%. Combined with the physiological and biochemical identification results, the strain L8 was identified as a strain of Lactobacillus gasseri, named Lactobacillus gasseri LG08, and its phys...
Embodiment 3
[0054] Example Lactobacillus trigerii LG08 degrades nucleosides and nucleotide product analysis
[0055] This example provides a method for detecting metabolites after degradation of adenosine, guanosine, inosine, adenosine, guanylate, inosine and 5'-guanylate disodium by Lactobacillus gasseri LG08 , including the following steps:
[0056] Add 5mmol / L adenosine, guanosine, inosine, adenylic acid, guanylic acid, inosine and 5'-guanylic acid disodium respectively in the ammonium acetate buffer solution, and the dry weight concentration of bacteria is 0.4g / L add PBS buffer solution to wash the bacteria cells, mix culture at 37°C for 2 hours, and then use ultrasonic crushing. Centrifuge for 15 min, take the supernatant and measure the concentration of metabolites (hypoxanthine, guanine, xanthine, uric acid) by mass spectrometry.
[0057] The mass spectrometry conditions are as follows: triple quadrupole mass spectrometer (Agilent 1290 series), reversed-phase chromatographic col...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com