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Application of sulfated glucuronic acid-xylose-rhamnose in enhancing immune function

A technology of glucuronic acid and rhamnosan, which is applied in the application field of enhancing immune function, can solve the problems of inconsistent structure and composition, unclear mechanism of action, and unreported activity of glycan immune regulation function, and achieve high production efficiency , The effect of simple preparation process steps

Pending Publication Date: 2020-02-07
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Kim et al. found that polysaccharides obtained by hot water extraction have immunomodulatory effects, but the mechanism of action is still unclear
Therefore, the present invention seeks to design and provide a sulfated glucuronic acid-xylo-rhamnolan application for enhancing immune function, and further degrades and separates the sulfated polysaccharide with a molecular weight of 17.3kD through the acid hydrolysis Glucuronic acid-xylo-rhamna oligosaccharides, the structure of the polysaccharides is inconsistent with that of the polysaccharides of Enteromorpha polysaccharides reported in the past, and the research on the activity of the polysaccharides in immune regulation has not been reported

Method used

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  • Application of sulfated glucuronic acid-xylose-rhamnose in enhancing immune function
  • Application of sulfated glucuronic acid-xylose-rhamnose in enhancing immune function
  • Application of sulfated glucuronic acid-xylose-rhamnose in enhancing immune function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The application of a kind of sulfated glucuronide-xylo-rhamnolan to enhance immune function involved in this embodiment is realized through the following technical scheme:

[0021] S1. Extraction of crude polysaccharides of Enteromorpha: Weigh 1000g of dry Enteromorpha, add 30L of 0.1M HCl to extract at room temperature for 4 hours to obtain an extract, then filter the extract through diatomaceous earth, neutralize and centrifuge to get the supernatant, The clear was concentrated and precipitated with ethanol. Determination of crude polysaccharide (EP) yield was 20.1%;

[0022] S2. DEAE-BioGel Agarose FF ion column chromatography crude polysaccharide: Take 8g of crude polysaccharide (EP) and grade it through DEAE-BioGel Agarose FF column chromatography, use different concentrations of NaCl as the eluent, collect the fractions, and obtain the water-washed fractions ( EP1), 0.3M NaCl fraction (EP2), 1M NaCl fraction (EP3) and 2M NaCl fraction (EP4), each fraction was col...

Embodiment 2

[0032] This example tests the effect of sulfated glucuronide-xylo-rhamnolan on activating macrophage RAW264.7 cells

[0033] In this example, the sulfated glucuronide-xylo-rhamnolan obtained in Example 1 is used, and the polysaccharide is treated in the form of an aqueous solution. The specific operation of the liquid preparation is as follows: Accurately weigh 50 mg of polysaccharide, dissolve it in 1 mL of 0.01M Phosphate buffer (pH 7.2-7.4), filtered through a needle filter with a pore size of 0.22 μm to obtain a sterile glycan stock solution, stored at -80°C, and used in the following examples.

[0034] The main operations are as follows:

[0035] Materials: Macrophage RAW264.7 cells, using DMEM high-glucose medium containing 10% fetal bovine serum, 100U / mL penicillin and 100U / mL streptomycin, placed at 37°C, containing 5% CO 2 cultured in an incubator.

[0036] (1) Griess reagent method to detect the release of NO in RAW264.7 cells

[0037] The RAW264.7 cells in the lo...

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Abstract

The invention relates to the field of biological medicine, in particular to application of sulfated glucuronic acid-xylose-rhamnose in enhancing immune function. The glycan is obtained by acidolysis of enteromorpha, and the monosaccharide mainly comprises glucuronic acid (Glca), xylose (Xyl), rhamnose (RHA) and the like. The preparation method comprises the following steps: room-temperature acidolysis, filtration, neutralization and concentration, ethanol precipitation, anion column chromatography, hydrolysis, ethanol precipitation, Bio-Gel P-10 Gel column chromatography, and freeze drying. The glycan prepared by the method has the characteristics of stable performance, low cost, high safety, high medicinal value and the like, and can significantly activate mouse peritoneal macrophages torelease nitric oxide. The glycan can be applied to preparation of medicines and health products of immunopotentiators.

Description

Technical field: [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of sulfated glucuronic acid-xylo-rhamnolan derived from marine enteromorpha to enhance immune function. Background technique: [0002] Immunity is an extremely complex physiological response, which is the function of the body's immune system to recognize and eliminate heterogeneous molecules, so as to maintain the body's physiological balance and stability. The body relies on this function to identify "self" and "non-self" components to fight against tissue damage and infection caused by pathological agents such as pathogens, toxic compounds or irradiation. This process involves the participation of inflammatory mediators, inflammation-related signaling pathways and inflammatory cytokines. [0003] There are rich biological resources in the ocean. The high salinity, high pressure, anoxic, low (constant) temperature, low (no) light and oligotrophi...

Claims

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Application Information

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IPC IPC(8): A61K31/737A61P37/02A23L33/105C08B37/00
CPCA61K31/737A61P37/02A23L33/105C08B37/0003C08B37/00A23V2002/00A23V2200/324A23V2250/51
Inventor 刘英娟郭云良金维华朱琳王潇璐王悦武筱林
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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