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Preparation method of high-purity stachyose

A stachyose, high-purity technology, applied in the field of stachyose, can solve the problems of low stachyose purity and unsatisfactory stachyose purity, and achieve the effects of facilitating large-scale industrial production, improving purity, and convenient operation

Inactive Publication Date: 2020-02-07
安徽民祯生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the purity of the stachyose product that existing preparation method obtains is not ideal enough, the purity of the stachyose that makes is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] A kind of preparation method of high-purity stachyose provided by the invention comprises the following steps:

[0025] 1) After washing the raw materials, dry them;

[0026] 2) adding the raw materials into water at 60-90°C to extract and squeeze the juice to obtain sugar liquid;

[0027] 3) adding yeast to the sugar solution, adjusting the pH value of the solution to 4-6, and aeration and fermentation at a temperature of 30-40°C for 20-30 hours;

[0028] 4) adding unslaked lime in fermented liquid until the solubility of unslaked lime reaches saturation;

[0029] 5) After the temperature is raised to 50-55°C, carbon dioxide is introduced to generate calcium carbonate precipitation;

[0030] 6) filter to remove impurities;

[0031] 7) passing the filtrate into a nanofiltration membrane for purification to obtain a purified solution;

[0032] 8) passing the purified solution into an ion exchange resin to obtain a stachyose solution;

[0033] 9) Concentrating and dr...

Embodiment 1

[0046] The preparation method of high-purity stachyose is characterized in that, comprising the following steps:

[0047] 1) Wash the Cordyceps ginseng and dry it;

[0048] 2) Put the Cordyceps root in water with 3 times the mass of the raw material, extract at 60°C for 2 hours, filter to separate the extract from the residue, and collect the extract; add water with twice the mass of the residue to the residue, and extract at 60°C for 2 hours , filtered, and mixed twice to obtain the sugar solution;

[0049] 3) Add yeast to the sugar solution, adjust the pH value of the solution to 4, and ventilate and ferment for 20 hours at a temperature of 30° C.; the amount of yeast added is 0.3% of the mass of the sugar solution;

[0050] 4) adding unslaked lime in fermented liquid until the solubility of unslaked lime reaches saturation;

[0051] 5) After the temperature is raised to 50° C., carbon dioxide is introduced to generate calcium carbonate precipitation; the amount of carbon ...

Embodiment 2

[0057] The preparation method of high-purity stachyose is characterized in that, comprising the following steps:

[0058] 1) After washing the snail dish and Luohan dish, dry them in the air;

[0059] 2) Add snail vegetables and Luo Hancai to water with 5 times the weight of the raw materials, extract at 90°C for 2 hours, filter to separate the extract from the residue, and collect the extract; Extract for 1 hour, filter, and mix the extracts twice to get the sugar solution; the mass ratio of snails and arhats is 1:1;

[0060] 3) Add yeast to the sugar solution, adjust the pH value of the solution to 6, and ventilate and ferment for 30 hours at a temperature of 40° C.; the amount of yeast added is 0.5% of the mass of the sugar solution;

[0061] 4) adding unslaked lime in fermented liquid until the solubility of unslaked lime reaches saturation;

[0062] 5) After the temperature is raised to 55° C., carbon dioxide is introduced to generate calcium carbonate precipitation; the ...

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Abstract

The invention relates to the field of stachyose, and discloses a preparation method of high-purity stachyose. The preparation method comprises the steps of performing leaching in water, performing squeezing to obtain a sugar solution, and adding yeast to the sugar solution, wherein the yeast can effectively decompose impurities of protein, glucose and the like in the sugar solution; adding quick lime to enable he impurities of resinite, colloid and the like in the sugar solution to be agglomerated, and inflating carbon dioxide to enable calcium carbonate and agglomerated impurities precipitate, so that impurity removal is facilitated, and the purity of the stachyose is improved; and performing treating through a nanofiltration film and ion exchange resins so as to obtain the high-purity stachyose. The preparation method is simple in steps and convenient to operate, the process is easy to control, and scaleable industrial production is convenient. Experiment results indicate that the yield of the stachyose can achieve 80.39%, and the purity is 92.36% or above.

Description

technical field [0001] The invention relates to the field of stachyose, in particular to a method for preparing high-purity stachyose. Background technique [0002] Stachyose is a naturally occurring tetrasaccharide, a functional oligosaccharide that can significantly promote the proliferation of beneficial bacteria such as Bifidobacteria. The pure product is a white powder with a slightly sweet taste and a sweetness of 22% of that of sucrose. It has a pure taste without any bad taste or peculiar smell. Its molecular structure: "galactose-galactose-glucose-fructose". [0003] Stachyose has a very obvious proliferation effect on beneficial bacteria such as bifidobacteria and lactobacilli in the human gastrointestinal tract, and can quickly improve the environment in the human digestive tract and regulate the balance of micro-ecological flora. It can promote the formation of beneficial bacteria in the digestive tract as dominant bacteria, inhibit the production of spoilage b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H3/06
CPCC07H1/08C07H3/06
Inventor 宋建民王德海宛荣生张琴王颂黄祥君
Owner 安徽民祯生物工程有限公司