Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for determining cholesterol by combining aqueous two-phase micellar system with ultra-high performance liquid chromatography

An ultra-high-efficiency liquid phase and two-phase water-phase technology is applied in the direction of measuring devices, instruments, scientific instruments, etc. It can solve the problems of the health impact of testing personnel, long processing time, and inconvenient operation, and achieves mild conditions, reduced process steps, The effect of easy operation

Active Publication Date: 2020-02-21
MARINE FISHERIES RES INST OF ZHEJIANG
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the problems of cumbersome steps, inconvenient operation and long processing time in the existing cholesterol detection method, which will affect the health of the test personnel, and provides a simple, green, accurate, fast, A method for the determination of cholesterol with a two-phase micellar system with high recovery rate combined with ultra-high performance liquid chromatography

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining cholesterol by combining aqueous two-phase micellar system with ultra-high performance liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) The muscle tissue of the aquatic product (large yellow croaker) was crushed into minced meat and used as a sample, and 1 mL of alkaline ionic liquid (1-butyl-3-methylimidazole hydroxide) was added to 0.1 g of the sample at a concentration of 0.3mol / L alkaline ionic liquid solution, catalyzed hydrolysis reaction at 75°C for 30min, centrifuged to get the supernatant;

[0034] (2) After mixing the ionic liquid (1-butyl-3-methylimidazolium imidazolium salt) and the non-ionic surfactant (Triton X-114) evenly, add deionized water until the total mass is 5g, stir and mix thoroughly Evenly, to obtain ionic liquid two-phase aqueous micelles, based on the total mass of the ionic liquid two-phase aqueous micelles system, the addition of the ionic liquid is 0.1%, and the addition of the nonionic surfactant is 3%;

[0035] (3) adding the supernatant in step (1) to the ionic liquid aqueous two-phase micelles in step (2) for shaking and mixing, standing at a constant temperature o...

Embodiment 2

[0045] (1) The muscle tissue of the aquatic product (shrimp) was crushed into minced meat and used as a sample, and 1 mL of alkaline ionic liquid (1-butyl-3-methylimidazole hydroxide) was added to 0.15 g of the sample at a concentration of 0.4mol / L alkaline ionic liquid solution, catalyzed hydrolysis reaction at 80°C for 20min, centrifuged to get the supernatant;

[0046] (2) After mixing the ionic liquid (1-butyl-3-methylimidazole chloride) and the non-ionic surfactant (Triton X-114) evenly, add deionized water until the total mass is 5g, stir and mix thoroughly Evenly, to obtain ionic liquid two-phase aqueous micelles, based on the total mass of the ionic liquid two-phase aqueous micelles system, the addition of the ionic liquid is 0.5%, and the addition of the nonionic surfactant is 5%;

[0047] (3) adding the supernatant in step (1) to the ionic liquid aqueous two-phase micelles in step (2) for shaking and mixing, standing at a constant temperature of 50° C. for 1.5 h to s...

Embodiment 3

[0057] (1) The muscle tissue of the aquatic product (portunus crab) was crushed into minced meat and then used as a sample, and 1 mL of alkaline ionic liquid (1-butyl-3-methylimidazole hydroxide) was added to 0.2 g of the sample at a concentration of 0.5 mol / L alkaline ionic liquid solution, catalyzed hydrolysis reaction at 90°C for 15 minutes, centrifuged to get the supernatant;

[0058] (2) After mixing the ionic liquid (1-butyl-3-methylimidazole acetate) and the non-ionic surfactant (Triton X-114) evenly, add deionized water until the total mass is 5g, and stir thoroughly Mix evenly to obtain ionic liquid two-phase aqueous micelles, based on the total mass of the ionic liquid two-phase aqueous micelles system, the addition of the ionic liquid is 1.0%, and the addition of the nonionic surfactant is 9%;

[0059] (3) adding the supernatant in step (1) to the ionic liquid aqueous two-phase micelles in step (2) for shaking and mixing, standing at a constant temperature of 60° C....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for determining cholesterol by combining an aqueous two-phase micellar system with ultra-high performance liquid chromatography. The method adopts basic ionic liquid catalysis to replace traditional saponification treatment to treat a sample through catalytic hydrolysis, extracts and separates the cholesterol by means of an aqueous two-phase micelle formed by an ionic liquid and a non-ionic surfactant, and combines UPLC for conducting cholesterol detection. The method is simple and convenient to operate, green, environment-friendly, precise, quick and high in recovery rate, and provides a scientific basis for extracting cholesterol in the substance by using the ionic liquid.

Description

technical field [0001] The invention relates to the technical field of aquatic product detection, in particular to a method for determining cholesterol in aquatic products by combining a two-phase micellar system with ultra-high performance liquid chromatography. Background technique [0002] Cholesterol, also known as cholesterol, is a derivative of cyclopentane polyhydrophenanthrene. Cholesterol is widely present in animals, especially in brain and nerve tissue, and is also high in kidney, spleen, skin, liver and bile. From a nutritional point of view, cholesterol is an indispensable and important substance for the human body. Most of it comes from its own synthesis, and a small part comes from dietary supplements. It not only participates in the formation of cell membranes, but also is the main raw material for the synthesis of bile acids, vitamin D and steroid hormones. . However, with the development of society and economy and the improvement of people's living standa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062G01N2030/067
Inventor 张小军朱俊向于丁一宋玉凤
Owner MARINE FISHERIES RES INST OF ZHEJIANG
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com