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Method for determining contents of vitamin K1 and vitamin K2 in modified milk powder

A method of determination, vitamin technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve simple steps, good reproducibility, accurate qualitative and quantitative results

Active Publication Date: 2020-02-21
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention provides a method for preparing vitamin K in milk powder 1 and vitamin K 2 Assay method to address vitamin K 1 and vitamin K 2 The problem of simultaneous and accurate measurement of content

Method used

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  • Method for determining contents of vitamin K1 and vitamin K2 in modified milk powder
  • Method for determining contents of vitamin K1 and vitamin K2 in modified milk powder
  • Method for determining contents of vitamin K1 and vitamin K2 in modified milk powder

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Embodiment 1

[0035] 1 Instruments and equipment

[0036] High performance liquid chromatography (with fluorescence detector, Waters, USA); Xbridge C18 column (3.5μm, 4.6×150cm, Waters, USA); zinc powder reduction column (50mm×4.6mm, Shanghai Anpu Experimental Technology Co., Ltd. company); 3-18K refrigerated centrifuge (Sigma, Germany); AB204-S electronic balance (Mettler Toledo, Germany); MS3 vortex mixer (IKA, Germany); SB-800DTD ultrasonic cleaner (Ningbo, China) Zhizhi Biotechnology Co., Ltd.); SW22 constant temperature water bath oscillator (Ningbo Xinzhi Biotechnology Co., Ltd., China); HGC-24 nitrogen blowing instrument (China Hengao Technology Co., Ltd.); Milli-Q ultrapure water system (USA Millipore Corporation).

[0037] 2 Materials and reagents

[0038] Methanol, n-hexane, dichloromethane (chromatographically pure, Merck, Germany); glacial acetic acid (chromatographically pure, Tianjin Concord Technology Co., Ltd.); absolute ethanol, zinc chloride, sodium chloride, sodium acet...

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Abstract

The invention discloses a method for determining contents of vitamin K1 and vitamin K2 in modified milk powder. The method comprises the following steps of: (1) extracting vitamin K1 and vitamin K2 inthe modified milk powder to be determined by adopting modes of enzymolysis and saponification to obtain a sample extracting solution; (2) detecting the vitamin K1 and the vitamin K2 in the sample extracting solution by adopting a post-column zinc powder reduction-high performance liquid chromatography-fluorescence detection method to obtain liquid chromatography data; and (3) according to the liquid chromatogram standard working curves of the vitamin K1 and the vitamin K2, obtaining the contents of the vitamin K1 and the vitamin K2 in the modified milk powder to be determined. The method is accurate in qualitative and quantitative analysis, high in sensitivity and good in reproducibility, and can meet the detection requirements of the vitamin K content in the modified milk powder.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for preparing vitamin K in milk powder 1 and vitamin K 2 method for the determination of content. Background technique [0002] Vitamin K is a kind of derivatives of naphthoquinone group with biological activity of phylloquinone. It participates in many functions such as hemostasis, bone metabolism, cell growth, and maintenance of cardiovascular health. It is an essential fat-soluble vitamin for the human body. Naturally occurring vitamins can be classified into vitamin K according to their side chain forms 1 (phylloquinone, VK1) and vitamin K 2 (menaquinones, VK2) are widely found in nature and are closely related to human metabolism. They are also the two most studied and applied compounds. Vitamin K 1 and vitamin K 2 All are fat-soluble vitamins, sensitive to light and alkali, heat-resistant, and have different intensities of biological activity in living...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/74
CPCG01N30/02G01N30/06G01N30/74G01N2030/067
Inventor 公丕学孙珊珊刘桂亮张艳侠薛霞王明栋刘艳明卢兰香
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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