Lipidomics analysis method of blood deficiency mouse model

A mouse model and analysis method technology, which is applied to the analysis of materials, scientific instruments, material separation, etc., can solve the problems of inability to measure low-abundance metabolites, small sample size, sensitivity, and low resolution, and achieve sample determination and The effect of high analysis efficiency, good repeatability, and simple pre-treatment

Inactive Publication Date: 2020-02-21
SHANXI UNIV
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Problems solved by technology

NMR and MS are the two main analytical platforms used in metabolomics. Sample pretreatment using NMR is relatively simple and requires a small sample volume, making it a powerful high-throughput technique that enables rapid analysis. A large number of samples, but its sensitivity, resolution is low, and it cannot measure low-abundance metabolites

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  • Lipidomics analysis method of blood deficiency mouse model
  • Lipidomics analysis method of blood deficiency mouse model
  • Lipidomics analysis method of blood deficiency mouse model

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Embodiment Construction

[0050] The lipidomics analysis method of the blood-deficiency mouse model in the present invention does not need standard compounds, and can quickly complete the accurate lipidomics analysis of the endogenous lipid molecules in the blood-deficiency mice.

[0051] Step 1, the construction and evaluation of blood deficiency mouse model, the specific steps are as follows:

[0052] On the first day, the blank control group was subcutaneously injected with 20 mg / kg of sterile water, and the model group was subcutaneously injected with 20 mg / kg of acetylphenylhydrazine;

[0053] In the morning of the fourth day, the blank control group was subcutaneously injected with 10 mg / kg of sterile water, the model group was subcutaneously injected with 10 mg / kg of acetophenhydrazine, and two hours later, the blank control group was intraperitoneally injected with 20 mg / kg of sterile water. Intraperitoneal injection of 20mg / kg of cyclophosphamide;

[0054] From the fourth day to the seventh d...

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Abstract

The invention discloses a lipidomics analysis method of a blood deficiency mouse model, and belongs to the technical field of analysis of endogenous lipid substance change in the blood deficiency mouse body. The lipidomics analysis method comprises the following steps: ultrasonically crushing spleen tissues of the blood deficiency model mouse to be detected by using methanol, methyl tert-butyl ether and ultrapure water, centrifuging and concentrating an extracting solution, redissolving the extracting solution with methanol and isopropanol, carrying out filtering and sample feeding, using a liquid phase Waters ACQUITY UPLC HSS T3 chromatographic column for separation, using a mobile phase A and a mobile phase B composed of acetonitrile, water, ammonium formate, formic acid and isopropanolfor elution, using a full-scanning mode of a high-resolution mass spectrometer for detection in a positive ion mode and a negative ion mode respectively, carrying out qualitative and relative quantitative analysis on measured data according to a chemical formula of target lipid molecules. The method has the advantages of being sensitive, accurate and independent from standard compounds, and can beefficiently suitable for lipidomics analysis on the blood deficiency mouse model.

Description

technical field [0001] The invention belongs to the technical field of analyzing the changes of endogenous lipid substances in blood-deficiency mice, and in particular relates to a lipidomics analysis method of a blood-deficiency mouse model. Background technique [0002] Blood deficiency refers to blood deficiency, viscera, meridian, body malnutrition, with pale or chlorotic complexion, pale lips, tongue, nails, dizziness, palpitations and dreams, numb hands and feet, women with less menstrual flow, pale complexion, late or menstrual period Closure, thready pulse, etc. are common syndromes. Traditional Chinese medicine believes that the causes of blood deficiency mainly include excessive blood loss, or prolonged illness due to the depletion of yin and blood, or dysfunction of the spleen and stomach, and the inability of water and grain essence to metabolize blood. [0003] Metabolomics is a research technique that can be used to study differential metabolites and disease m...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8813
Inventor 刘月涛李鑫琦秦雪梅
Owner SHANXI UNIV
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