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A method for separation and purification of Atractylodes oligosaccharides

A technology for separation and purification of oligosaccharides, applied in the field of natural medicinal chemistry, can solve the problems of difficult oligosaccharide components, difficult to obtain pure products, high polarity, etc., achieve significant immune activity, improve separation and purification effects, increase yield and The effect of purity

Active Publication Date: 2022-02-15
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fructooligosaccharides in Atractylodes atractylodes has good biological activity and low toxicity, but its separation and purification are difficult
First of all, oligosaccharide components have no UV absorption, and common UV detectors cannot detect them, so it is difficult to track and detect oligosaccharide components during the purification process
In addition, the solubility properties of oligosaccharides with different degrees of polymerization are quite different, and a small amount of samples may be precipitated during the separation and purification process, and the polarity of the oligosaccharide components is relatively high. It is difficult to obtain pure products from general separation materials. Therefore, it is impossible Obtain high purity oligosaccharides

Method used

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  • A method for separation and purification of Atractylodes oligosaccharides
  • A method for separation and purification of Atractylodes oligosaccharides
  • A method for separation and purification of Atractylodes oligosaccharides

Examples

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Effect test

Embodiment 1

[0025] A method for separating and purifying Atractylodes oligosaccharides, comprising the steps of:

[0026] (1) Extraction: add 1 ± 0.01g of Atractylodes atractylodes powder into 20mL of 60v% ethanol aqueous solution, heat to reflux extraction, the extraction temperature is 85°C, and the extraction time is 2.5h to obtain the crude extract of Atractylodes oligosaccharides;

[0027] (2) Decolorization: use D101 macroporous resin column to decolorize the crude extract of Atractylodes oligosaccharides, wash 3 column volumes with deionized water, collect the eluate and rotary evaporate at 60 °C, and then freeze-dry to obtain crude Atractylodes atractylodes Oligosaccharide powder, the yield is 18.65%;

[0028] Take 0.02±0.0001g Atractylodes crude oligosaccharide powder, dissolve it in pure water, and use high-performance liquid chromatography for detection. The conditions of high-performance liquid chromatography are: mobile phase: acetonitrile in phase A; Column temperature: 30°...

Embodiment 2

[0037] A method for separating and purifying Atractylodes oligosaccharides, comprising the steps of:

[0038] (1) Extraction: Add 1 ± 0.01g of Atractylodes atractylodes powder into 25mL of 80v% ethanol aqueous solution, heat to reflux extraction, the extraction temperature is 80°C, and the extraction time is 2.5h, to obtain the crude extract of Atractylodes oligosaccharides;

[0039] (2) Decolorization: use D101 macroporous resin column to decolorize the crude extract of Atractylodes oligosaccharides, wash 3 column volumes with deionized water, collect the eluate and rotary evaporate at 60 °C, and then freeze-dry to obtain crude Atractylodes atractylodes Oligosaccharide powder, the yield is 17.54%;

[0040](3) Separation and purification of DP3-DP8: Separation and purification of the crude oligosaccharide powder of Atractylodes atractylodes is carried out by size exclusion chromatography. Gel P-2, using ultrapure water as the mobile phase for isocratic elution, the flow rate ...

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Abstract

The invention relates to a method for separating and purifying Atractylodes atractylodes oligosaccharides, which belongs to the technical field of natural medicine chemistry. Firstly, Atractyrrhizae powder is added to 40-100v% ethanol aqueous solution at a material-to-liquid ratio of 1:10-1:25, and heated to reflux Extraction, degreasing, decolorization and freeze-drying to obtain Atractylodes crude oligosaccharide powder, then use molecular exclusion chromatography and hydrophilic interaction chromatography to separate and purify the crude oligosaccharides, and use high performance liquid phase tracking detection to obtain different polymerization degrees Pure Atractylodes oligosaccharides. The method of the invention can effectively improve the separation and purification effect of the oligosaccharide, and improve the yield and purity of the oligosaccharide. The atractylodes oligosaccharide prepared by the invention has significant immune activity and can be used to prepare immune-promoting drugs.

Description

technical field [0001] The invention belongs to the technical field of natural medicinal chemistry, and in particular relates to a method for separating and purifying Atractylodes oligosaccharides. Background technique [0002] Atractylodes is the dry rhizome of Atractylodes lancea (Thunb) DC or Atractylodeschinensis Koidz of Compositae. Modern pharmacological studies have proved that Atractylodes atractylodis has unique chemical components and significant pharmacological activities, and has anti-ulcer, anti-arrhythmia, blood pressure-lowering, diuretic, liver-protecting, anti-inflammatory, and antibacterial effects. At present, most researches on the active ingredients of Atractylodes atractylodes are volatile oils, but in clinical medicine, Atractylodes atractylodes is widely used in the form of water decoction and has good curative effect, which shows that the water-soluble components of Atractylodes atractylodes have good pharmacological activity and application value. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/06
CPCC08B37/0051C08B37/0003C07H1/08C07H3/06
Inventor 吕广萍张盈月黄和庄丹王慧阳秦靖刘春尧
Owner NANJING TECH UNIV