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Application of oxidized konjac microspheres to food or drug delivery system

A technology of microspheres and food, which is applied in the biological field and can solve problems that need to be studied

Active Publication Date: 2020-03-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, currently microsphere carriers formed from konjac glucomannan remain to be studied

Method used

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  • Application of oxidized konjac microspheres to food or drug delivery system
  • Application of oxidized konjac microspheres to food or drug delivery system
  • Application of oxidized konjac microspheres to food or drug delivery system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] In this example, sulfhydryl-modified oxidized konjac glucomannan OKGM-Cys was prepared according to the following method:

[0120] Weigh 3 parts of 100mg oxidized konjac glucomannan and dissolve them in 20mL of ultrapure water respectively. After the oxidized glucomannan is completely dissolved, add 0.083g of EDC [1-(3-dimethylaminopropyl)- 3-Ethylcarbodiimide hydrochloride] Activated for 1 hour, then added 0.038 g of cysteine ​​hydrochloride, and reacted on a magnetic stirrer at room temperature for 24 hours, during which nitrogen gas was introduced to remove oxygen.

[0121] The product was precipitated with an equal volume of ethanol, filtered, and the precipitate was washed 3 times with ethanol to remove EDC and unreacted cysteine ​​hydrochloride to obtain precipitated OKGM-Cys. Reconstitute the precipitate with 10 mL of ultrapure water, and stir at room temperature for 2 h until no visible white particles are seen, which can be considered as complete dissolution of...

Embodiment 2

[0123] In this example, microsphere carriers were prepared as follows:

[0124] Take 50 mg of the thiol-modified oxidized konjac glucomannan powder prepared in Example 1 and 6 mg of ferrous sulfate heptahydrate and dissolve them in 500 microliters of deionized water. After the powder is completely dissolved, dissolve 0.75 g of span 80 into 20 g of paraffin oil In, stir well. The water phase is gradually added to the oil phase under mild conditions to form a water-in-oil emulsion. Under the condition of a water bath at 35°C, air was gradually pumped into the emulsion, and at the same time, it was stirred at a speed of 500 rpm under a magnetic stirrer for 4 hours. After 4 hours, under the centrifugation condition of 3000 rpm and 2 min, the microparticles were washed with n-hexane for 3 times, and the microparticles were washed with methanol for 3 times. The microsphere precipitate washed in the last step was dissolved in 2 mL of ultrapure water with pH 3, and the sample was st...

Embodiment 3

[0130] To characterize the stability of the microsphere carrier obtained in Example 2, Comparative Examples 1 and 2 under simulated gastrointestinal fluid in vitro, the specific steps are as follows:

[0131] Dissolve 1 g of pepsin in 90 mL of ultrapure water, adjust the pH of the solution to 1.2 with hydrochloric acid solution, and set the volume to 100 mL to obtain 1% (w / v) simulated gastric juice; take 0.68 g of potassium dihydrogen phosphate, add Dissolve it in 50 mL of ultrapure water, adjust the pH of the solution to 6.8 with 0.5 mol / L sodium hydroxide, add 1 g of trypsin, and dilute to 100 mL to obtain 1% (w / v) simulated intestinal juice.

[0132] Take 100 μL of the three kinds of OKGM-Cys thiolated oxidized konjac glucomannan microspheres prepared above and add them to 400 μL of simulated gastric juice, and digest them in a water bath at 37°C for 30 min; Glucomannan microspheres were added to 400 μL of simulated intestinal fluid, and digested in a water bath at 37°C fo...

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Abstract

The invention brings forward a microsphere carrier, a method for preparing the microsphere carrier, an embedding object, a medicine, a food, and the application of the embedding object in preparing the medicine or the food. The microsphere carrier comprises thiol-modified oxidized konjac glucomannan. The microsphere carrier of the invention has strong stability in gastric juice and high adhesion performance in intestinal tract, so that functional factors embedded on the microsphere carrier have higher bioavailability.

Description

technical field [0001] The present invention relates to the field of biology. Specifically, the present invention relates to the application of oxidized konjac microspheres in food or drug delivery systems. More specifically, the present invention relates to microsphere carriers, methods for preparing microsphere carriers, embeddings, medicines, foods, and uses of embeddings in the preparation of medicines or foods. Background technique [0002] Polysaccharides are the most diverse and important biopolymers on earth. Various embedding systems have been established based on different types of polysaccharides, such as microsphere carriers based on starch, chitosan, and sodium alginate. Among them, Konjac Glucomannan (KGM), as a natural prebiotic glucomannan, can be extracted from the perennial herb Konjac. It is a heteroglucomannan formed by linking β-D-mannose and β-D-glucose through β-1,4- or β-1,3-glycosidic bonds. Since there is no enzyme to digest this konjac glucomann...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/36A61K31/7105A61P35/00A61P1/00A61P3/04A23L19/10A23P10/30
CPCA23V2002/00A61K9/5036A61K31/7105A23L19/115A23P10/30A61P1/00A61P3/04A61P35/00A23V2200/308A23V2200/32
Inventor 李媛任发政刘莹于政权
Owner CHINA AGRI UNIV
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