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Seaweed cracking method and application thereof in fermentation hydrogen production

A kind of seaweed, cracking liquid technology, applied in the direction of fermentation, can solve the problems of refractory brown macromolecular substances, consumption of organic matter, long reaction time, etc., to achieve cheap and easy to obtain substrate sources, promote full release, and eliminate inhibition. Effect

Inactive Publication Date: 2020-03-06
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have found that it is difficult to effectively crack algae cells and release organic matter by single acid, alkali treatment and ultrasonic treatment.
In heat treatment, only when the temperature is high (greater than 121-180°C), can the seaweed be effectively decomposed, but high temperature is accompanied by an increase in energy consumption, and Maillard reaction will occur during the reaction process, making reducing sugar and Protein polymerization and condensation reactions not only consume organic matter that can be used for hydrogen production, but also form refractory brown macromolecules
Although the reaction conditions of enzymatic hydrolysis treatment are mild, the reaction time required is long, and the enzyme preparation is expensive, so it is difficult to realize the popularization and application in engineering
In addition, the phenolic acid contained in the seaweed cracking solution has antibacterial effect, which will inhibit the activity of hydrogen-producing microorganisms, thereby reducing the hydrogen production effect of the system. Degradation of phenolic acids

Method used

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  • Seaweed cracking method and application thereof in fermentation hydrogen production
  • Seaweed cracking method and application thereof in fermentation hydrogen production
  • Seaweed cracking method and application thereof in fermentation hydrogen production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The cracking effect of 10-30kGy dose irradiation on seaweed:

[0029] Take 30g of dried and pulverized seaweed particles and place them in a 1L ground-mouth bottle, add 970mL of deionized water to it to obtain a mixed solution with a seaweed concentration of 30g TS / L. Prepare 3 bottles of seaweed and water mixture in the same way. The three mixtures were irradiated with gamma rays at doses of 10, 20 and 30 kGy at room temperature, and the radiation source was 60 Co. After the irradiation, the seaweed cracking solution was obtained; the indicators in the untreated seaweed and the seaweed cracking solution treated with each irradiation dose were analyzed, and the cracking effect of the seaweed was obtained as shown in Table 1.

[0030] Table 1 The cracking effect of 10-30kGy dose irradiation on seaweed

[0031]

[0032] It can be seen from Table 1 that the released amount of SCOD, polysaccharide and protein increases with the increase of ionizing radiation dose in s...

Embodiment 2

[0034] 0-30kGy dose irradiation with 2% H 2 SO 4 The cracking effect of coupling processing seaweed:

[0035] Take 30g of dried and pulverized seaweed particles and place them in a 1L ground-mouth bottle, add 970mL of deionized water to it to obtain a mixed solution with a seaweed concentration of 30g TS / L. Prepare 4 bottles of seaweed and water mixture in the same way. Add 2% concentrated H of the mixed solution quality of seaweed and water to 4 bottles of mixed solution 2 SO 4 , and received γ-ray irradiation with doses of 0, 10, 20 and 30kGy at room temperature, and the radiation source was 60 Co. After the irradiation, adjust the pH of the mixed solution to 7.0 with NaOH to obtain the seaweed cracking solution; analyze the untreated seaweed and each irradiation dose with H 2 SO 4 The results of seaweed cracking are shown in Table 2 by coupling the various indicators in the obtained seaweed cracking solution.

[0036] Table 2 0-30kGy dose irradiation with 2% H 2 SO...

Embodiment 3

[0040] 0-30kGy dose irradiation coupled with 2% NaOH cracking effect of seaweed treatment:

[0041] Take 30g of dried and pulverized seaweed particles and place them in a 1L ground-mouth bottle, add 970mL of deionized water to it to obtain a mixed solution with a seaweed concentration of 30g TS / L. Prepare 4 bottles of seaweed and water mixture in the same way. Add 2% NaOH powder of seaweed and water mixed solution to 4 bottles of mixed solution, and accept gamma ray irradiation with doses of 0, 10, 20 and 30 kGy respectively at room temperature, and the radioactive source is 60 Co. After the irradiation, use H 2 SO 4 Adjust the pH of the mixed solution to 7.0 to obtain the seaweed cracking solution; analyze the untreated seaweed and various indicators in the seaweed cracking solution coupled with each irradiation dose and NaOH, and obtain the cracking effect of the seaweed as shown in Table 3.

[0042] Table 3 Cracking effect of 0-30kGy dose irradiation and 2% NaOH coupled...

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Abstract

The invention discloses a seaweed cracking method and an application of the cracking method in fermentation hydrogen production, which belong to the technical field of biological fermentation hydrogenproduction. According to the method, seaweed is treated by coupling acid or alkali and ionization irradiation to obtain seaweed cracking liquid; according to the invention, full release of organic components in seaweed can be effectively promoted; the obtained seaweed cracking liquid is used as a substrate for efficient hydrogen production, a cheap and easily available substrate source is provided for a biological hydrogen production process, and the cost of fermentation hydrogen production is reduced.

Description

technical field [0001] The invention belongs to the technical field of hydrogen production by biological fermentation, and in particular relates to a method for cracking seaweed and its application in hydrogen production by fermentation. Background technique [0002] Hydrogen has the advantages of high calorific value, clean combustion products, easy storage and transportation, etc. It has a wide range of uses and is an ideal clean energy. There are many ways to prepare hydrogen, and the traditional preparation methods often rely on fossil energy, which reduces the environmental benefits of hydrogen. In contrast, biohydrogen production has the advantages of mild reaction conditions and renewable energy sources. In particular, hydrogen production by dark fermentation, which has high hydrogen production efficiency and can use a variety of organic substances as substrates, has received extensive attention. [0003] The choice of substrate directly determines the operating cos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00
CPCC12P3/00C12P2201/00C12P2203/00
Inventor 王建龙阴亚楠
Owner TSINGHUA UNIV
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