High-flux ultra-sensitive fluorescent gold nano-cluster immunochromatographic test strip and application thereof

A technology of fluorescent gold nanometers and test strips, which can be used in measuring devices, analytical materials, instruments, etc., and can solve the problem of low fluorescence quantum yield
CN110873793AInactive Publication Date: 2020-03-10CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2020-03-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a high-flux ultra-sensitive fluorescent gold nano-cluster immunochromatographic test strip and application thereof. The fluorescent gold nano-cluster immunochromatography teststrip comprises a sample pad, a nitrocellulose membrane, absorbent paper and a PVC viscous bottom plate; the nitrocellulose membrane is coated with two target object detection lines and a quality control line. The invention also discloses a complete set of fluorescent gold nano-cluster coupling antibody probe, which is composed of a streptavidin fluorescent gold nano-cluster labeled biotinylated anti-small molecule compound A antibody and a streptavidin fluorescent gold nano-cluster labeled biotinylated anti-small molecule compound B antibody. The fluorescent gold nano-cluster is a green fluorescent gold nano-cluster which takes 6-aza-2-thiopyrimidine and arginine as ligands and has super-strong fluorescence. The test strip has the advantages that the method is simple and rapid, the resultis visual and accurate, quantitative / semi-quantitative detection can be achieved, and high-flux ultra-sensitive online detection of veterinary drug residues can be achieved.
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Description

technical field

[0001] The invention belongs to the field of veterinary drug detection in food safety, and in particular relates to a high-throughput ultrasensitive fluorescent gold nano-cluster immunochromatographic test strip and an application thereof. Background technique

[0002] Immunochromatography technology is a unique immune analysis method that appeared in the early 1980s. It has the advantages of fast, simple, sensitive and convenient, and low price. It can realize online rapid detection and monitoring of target objects. It is currently used in food safety and medical diagnosis , environmental monitoring and other fields have been widely used. The immunochromatographic technique based on colloidal gold as a marker is the most successful and widely used rapid screening method, but its sensitivity is often difficult to meet people's requirements. Therefore, it is necessary to establish a high-throughput and ultra-sensitive immunochromatographic method for the simu...

Claims

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