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Sensitive probe, method for detecting tetracycline and applications

A tetracycline and probe technology, applied in the field of sensitive probes, can solve the problems of low sensitivity of immunochromatographic detection, and achieve the effects of easy modification of functional groups, good specificity and large specific surface area

Pending Publication Date: 2020-03-17
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the defects and deficiencies in the prior art, the present invention provides a sensitive probe, a tetracycline detection method and its application to solve the technical problem of low sensitivity of immunochromatography detection

Method used

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  • Sensitive probe, method for detecting tetracycline and applications
  • Sensitive probe, method for detecting tetracycline and applications
  • Sensitive probe, method for detecting tetracycline and applications

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0039] Following the above-mentioned technical scheme, this embodiment provides a sensitive probe MoS 2-Abs and its preparation method, including using molybdenum disulfide nanosheet as a signal carrier, combined with tetracycline monoclonal antibody to form a sensitive probe, specifically including the following steps:

[0040] (1) Preparation of signal carrier

[0041] will contain 2g MoS 2 A 400mL mixed aqueous solution of powder and 0.6g sodium cholate was ultrasonically treated at room temperature for 20h. Ultrasonic waves generated cavitation bubbles, which were helpful for high-energy jets, destroyed massive crystals, and formed a dark green dispersion solution. MoS 2 The crystals are exfoliated longitudinally into 2D thin nanosheets, which are stably dispersed in the surfactant solution.

[0042] Using the differential zonal centrifugation pathway, MoS can be 2 size fractionation and removal of the active agent. Graded MoS 2 The sizes are: 450~550nm (L-MoS 2 ), ...

Embodiment 2

[0047] Embodiment 2: Preparation of immunochromatographic test strips for rapid detection of tetracycline antibiotics

[0048] A preparation method for a high-sensitivity immunochromatographic test strip for rapid detection of tetracycline, comprising the following steps:

[0049] The immunochromatographic test strip sensor consists of five parts, including a backing plate, a nitrocellulose (NC) membrane, a binding pad, a sample pad and an absorbent pad. First, the conjugation pads and sample pads made of glass fibers were soaked in 2% bovine serum albumin to seal the microwells and dried overnight at 37 °C. Next, 1 mg / mL antigen (TET-BSA tetracycline-bovine serum albumin conjugate) and 1 mg / mL goat anti-mouse immunoglobulin were coated on the NC membrane at a streak rate of 1 μL / cm as detection (T) line and control (C) line, the distance between detection (T) line and control (C) line is 5mm. Attach the NC membrane, binding pad, sample pad and absorbent pad to the liner in ...

Embodiment 3

[0050] Example 3: Performance evaluation of immunochromatographic test strips for rapid detection of tetracycline

[0051] Performance evaluation of the immunochromatographic test strip for rapid detection of tetracycline as described above:

[0052] (1) Sensitivity determination

[0053] Tetracycline was dissolved in deionized water, and serially diluted to make test solutions with different concentrations within the range of 0.012-3 ng / mL, and deionized water was used as a blank control. Dip the sample pad of the test strip into the MoS-containing 2 - In 100 μL test solution of Abs probe, after immunochromatography, the visual results were observed within 11 min. For sensitivity evaluation, these standard solutions were detected by ordinary test strips and newly developed test strips, respectively. When the T line was observed by the naked eye to be significantly lighter than the negative control strip, the corresponding minimum tetracycline concentration was defined as t...

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Abstract

The invention discloses a sensitive probe, a method for detecting tetracycline and applications. The sensitive probe comprises a signal carrier and a tetracycline monoclonal antibody, wherein the signal carrier is a molybdenum disulfide nanosheet, and the particle sizes of the molybdenum disulfide nanosheet are 450-550 nm, 200-300 nm and 40-60 nm. In immunodetection, the two-dimensional molybdenumdisulfide nanosheet is used as the signal carrier to construct the probe, control of a very limited amount of antibodies is the key for improving sensitivity, the specific surface area of molybdenumdisulfide is large, the surface is easy in functional group modification, the adsorption capacity is high, the biocompatibility is good, a small amount of antibody-labeled molybdenum disulfide servesas a detection probe to realize high-sensitivity target object detection, a naked eye detection limit can be as low as 0.023 ng / mL, the sensitivity is improved by at least 100 times compared with thatof a traditional gold-labeled test strip, and the sensitive probe can be successfully applied to tetracycline detection in milk, honey and beef samples.

Description

technical field [0001] The invention belongs to the field of biological detection, and relates to a sensitive probe, a tetracycline detection method and its application, in particular to a sensitive probe that uses molybdenum disulfide nanosheets as a signal carrier and marks a monoclonal antibody, and can be used together with a test strip to rapidly Detection and application of tetracycline antibiotics. Background technique [0002] The excessive use of tetracycline and other antibiotics has caused serious drug residues in meat products and seafood products, and has become a food safety hazard that endangers human health. At present, the qualitative and quantitative detection of tetracycline at home and abroad is more classical. Liquid chromatography -Mass Spectrometry / Mass Spectrometry (LC-MS / MS), Gas Chromatography-Mass Spectrometry (GC-MS), High Performance Liquid Chromatography (HPLC), although these methods have high accuracy, the instruments are expensive and the pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/53G01N33/551
CPCG01N33/9446G01N33/577G01N33/558G01N33/551
Inventor 王丽贾珮补彤李睿孙新玉刘英男
Owner NORTHWEST A & F UNIV
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