High-sensitivity D-dimer detection kit and use method thereof

Abstract

The invention discloses a high-sensitivity D-dimer detection kit and a use method thereof. The kit comprises a kit main body, a sealing cover, a protective cover and a reagent, wherein a solid phase carrier hole, a sample hole, a detection antibody hole, a fluorescein hole, a washing hole and a reading hole are sequentially formed in the kit main body; the kit comprises all reagents required for completing immunodetection, the end part of a solid-phase carrier loaded in the kit is coated with a capture antibody, and the solid-phase carrier is immersed into each hole in the kit, so that the combination of a target antigen in a detected sample and subsequent detection steps can be completed, and the manufacturing and maintenance costs are greatly reduced; the surface of the solid-phase carrier is coated with the polysaccharide compound with biocompatibility, the polysaccharide compound can form hydrogen bond weak interaction with protein molecules, and specific immune signals are effectively enhanced.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a high-sensitivity D-dimer detection kit and a use method thereof. Background technique [0002] D-dimer is derived from the cross-linked fibrin clot dissolved by plasmin, which is a specific degradation product produced by fibrin monomer cross-linked by activated factor XIIIa and then hydrolyzed by plasmin. markers of the fibrinolytic process. [0003] Under physiological conditions, the body maintains a dynamic balance of coagulation and fibrinolysis to ensure the timely formation and removal of fibrin, while the normal level of D-dimer in the human body is generally below 200 μg / l. If the coagulation and fibrinolysis systems are activated in vivo , the level of D-dimer will increase, so D-dimer can be used as one of the molecular markers of hypercoagulable state and hyperfibrinolysis in vivo, and the specificity of D-dimer reflects the presence of blood coagulation and fibri...

AI Technical Summary

Problems solved by technology

Latex agglutination method is simple and fast, suitable for point-of-care testing (POCT), but can only be used for qualitative determination, mostly for screening; ELISA method has high sensitivity, but poor quantitative accuracy, long operation time, and low automation, so it is not suitable for POCT Necessary; latex particle-enhanced immune turbidimetry can be easily applied to automatic biochemical instruments, but it requires instruments, takes a long time, and is suitable for processing a large number of samples. For emergency departments and primary hospitals, it cannot meet the purpose of rapid detection; immunogold The standard method is similar to the latex agglutination method, which has the advantages of simple and fast operation, but also has poor quantitative accuracy, especially poor repeatability, which limits its clinical application, especially not suitable for accurate quantification to help diagnose diseases. Quantitative detection of diagnostic body fluid marker proteins

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