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Effective remediation method for heavy metal soil pollution

A technology of soil pollution and remediation method, which is applied in the field of remediation of heavy metal soil pollution, can solve the problems of small biomass, slow growth, weak adaptability, etc., and achieve the effect of increasing biomass, promoting growth and improving resistance

Inactive Publication Date: 2020-04-03
JIANGXI PURUIFENG ECOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its efficiency in remediating soil heavy metal pollution is still limited by slow growth, small biomass, weak adaptability and the availability of heavy metals in the soil.
The endophytic bacteria isolated from the superaccumulative plant Solanum nigrum can colonize the plant body of Solanum nigrum, promote the growth of Solanum nigrum, resist stress, increase biomass, and absorb, transport, and accumulate heavy metals in soil. This is of great significance for the practical application of the application of Solanum nigrum to remediate heavy metal-contaminated soil. However, in the prior art, there is no specific report on the use of the hyperaccumulative plant Solanum nigrum and its endophytic bacteria to jointly remediate heavy metal-contaminated soil.

Method used

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  • Effective remediation method for heavy metal soil pollution
  • Effective remediation method for heavy metal soil pollution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of endophytes:

[0032] a, plant pretreatment: rinse the collected fresh nightshade with clear water and cut its roots, stems and leaves into 2cm sections;

[0033] b. Surface disinfection: Soak the pretreated roots, stems, and leaf segments in 70% ethanol for 3 minutes, then soak them in 2% sodium hypochlorite for 3 minutes, and finally rinse them with sterile water several times, and take the last lotion and apply it on a plate Carry out constant temperature culture as the control of surface disinfection, if no microorganisms grow out on the control plate, it shows that the disinfection is complete, and all the isolates obtained in the subsequent steps are endophytic bacteria of Solanum nigrum;

[0034] c. Isolation and leaching of endophytes: the surface-sterilized root, stem, and leaf segments were placed in 10 mL of sterilized PBS containing 1% cellulase and 0.25% Tween80 under sterile conditions. Fully grind in the solution, transfer the grinding ...

Embodiment 2

[0043] (1) Preparation of endophytes:

[0044] a, plant pretreatment: rinse the collected fresh nightshade with clear water and cut its roots, stems and leaves into 2cm sections;

[0045] b. Surface disinfection: Soak the pretreated roots, stems, and leaf segments in 70% ethanol for 3 minutes, then soak them in 2% sodium hypochlorite for 3 minutes, and finally rinse them with sterile water several times, and take the last lotion and apply it on a plate Carry out constant temperature culture as the control of surface disinfection, if no microorganisms grow out on the control plate, it shows that the disinfection is complete, and all the isolates obtained in the subsequent steps are endophytic bacteria of Solanum nigrum;

[0046] c. Isolation and leaching of endophytes: the surface-sterilized root, stem, and leaf segments were placed in 10 mL of sterilized PBS containing 1% cellulase and 0.25% Tween80 under sterile conditions. Fully grind in the solution, transfer the grinding ...

Embodiment 3

[0055] (1) Preparation of endophytes:

[0056] a, plant pretreatment: rinse the collected fresh nightshade with clear water and cut its roots, stems and leaves into 2cm sections;

[0057] b. Surface disinfection: Soak the pretreated roots, stems, and leaf segments in 70% ethanol for 3 minutes, then soak them in 2% sodium hypochlorite for 3 minutes, and finally rinse them with sterile water several times, and take the last lotion and apply it on a plate Carry out constant temperature culture as the control of surface disinfection, if no microorganisms grow out on the control plate, it shows that the disinfection is complete, and all the isolates obtained in the subsequent steps are endophytic bacteria of Solanum nigrum;

[0058] c. Isolation and leaching of endophytes: the surface-sterilized root, stem, and leaf segments were placed in 10 mL of sterilized PBS containing 1% cellulase and 0.25% Tween80 under sterile conditions. Fully grind in the solution, transfer the grinding ...

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Abstract

The invention provides an effective remediation method for heavy metal soil pollution. The effective remediation method comprises the following steps: (1) preparing endophyte, namely cleaning a solanum nigrum plant sample, cutting into small sections, carrying out surface disinfection, grinding, separating and leaching the endophyte, diluting an endophyte leachate, coating a flat plate with the diluted endophyte leachate, carrying out streak culture until the endophyte is pure, and screening to obtain serratia endophyte; (2) remediation of heavy metal contaminated soil: a, inoculating endophyte: soaking the roots of solanum nigrum seedlings into an endophyte suspension, and culturing for 1-2 days; b, applying a soil conditioner, specifically, controlling the thickness to be 15-25cm, spraying and watering, and ploughing uniformly; c, planting: planting solanum nigrum inoculated with endophyte in the heavy metal contaminated soil, watering, and applying urea; d, mowing: carrying out light mowing on solanum nigrum, and reserving stubbles of 30cm. according to the method, hyper-accumulation plant solanum nigrum is inoculated with the endophyte to jointly repair the heavy metal contaminated soil, the heavy metal contaminated soil can be quickly and effectively repaired, and the method is simple, easy to implement and low in maintenance cost.

Description

technical field [0001] The invention relates to the field of soil remediation, in particular to an effective remediation method for heavy metal soil pollution. Background technique [0002] Soil is the resource that human beings rely on for survival, and it is also the most recyclable resource in nature. With the acceleration of urbanization and industrialization, the problem of heavy metal polluted soil in my country has become a focal environmental issue that cannot be ignored. The remediation methods of heavy metal soil pollution usually include physical control, chemical and biological remediation methods. Physical control is to use the principles of physics or physical chemistry to control heavy metal soil pollution. There are mainly methods such as foreign soil, soil replacement, soil upturning, topsoil removal, leaching, heat treatment, and electrolysis. The disadvantages are complicated operations and high treatment costs. And it is easy to cause the decrease of soi...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/105
Inventor 刘云文阳平
Owner JIANGXI PURUIFENG ECOLOGICAL TECH CO LTD
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