Pretreatment method of epothilone B fermentation liquor

A technology of epothilone and fermented liquid, applied in the direction of organic chemistry, etc., can solve the problems affecting the quality of epothilone B final product, cumbersome plate and frame filtration or centrifugation operation, easy to block resin column sieve plate, etc., and achieve improvement Extraction efficiency and yield, saving column time, saving organic solvent effect
CN110964029AActive Publication Date: 2020-04-07LUNAN PHARMA GROUP CORPORATION
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
LUNAN PHARMA GROUP CORPORATION
Publication Date
2020-04-07

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Abstract

The invention belongs to the technical field of biology, and particularly discloses a pretreatment method of epothilone B fermentation liquor. An inorganic salt is added into the fermentation liquor,so that resin and thalli are thoroughly separated, only the resin phase remains after the thalli are removed, the subsequent extract is reduced to 1 / 3 of the original mixture, an organic solvent for further extraction is saved, and impurities in the thalli are prevented from being introduced into the subsequent purifying process; and impurities with the retention time similar to that of epothiloneB are removed from the source. In addition, the separated resin phase does not need to be centrifuged or filtered by a plate frame and is directly loaded into a column without blocking a sieve plate,so that the column passing time is saved. According to the pretreatment method of the epothilone B fermentation liquor, the thalli and the resin are well separated, subsequent extraction steps are simplified, the usage amount of subsequent extraction organic solvents is reduced, the extraction efficiency and yield are improved, the universality is high, and the industrialization cost is low.
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Description

technical field

[0001] The invention belongs to the technical field of bioseparation, and in particular relates to a pretreatment method of a macrolide antitumor drug epothilone B fermented liquid. Background technique

[0002] Epothilones (epotuilones) were discovered in the secondary metabolites of the myxobacteria Sorangium cellulosum in 1993. Its structure is based on the sixteen-membered lactone macrocycle as the center body and the thiazole ring ligand as the side chain. A class of cytotoxic compounds, an antineoplastic drug with tubulin polymerization and microtubule stabilizing effects similar to paclitaxel. Paclitaxel has achieved great success at the end of the 20th century, but its source is extremely scarce, its own toxic side effects and multidrug-resistant cell lines and other factors also limit its use. Compared with paclitaxel, epothilone has no restriction of plant source, no disadvantages such as complex structure and water insolubility of paclitaxel, and ...

Claims

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