Selenium-rich lactic acid bacteria preparation and preparation method thereof

A technology of lactic acid bacteria and preparations, applied in the directions of biochemical equipment and methods, microorganism-based methods, bacteria, etc., can solve problems such as limited guiding significance, and achieve the effect of increasing the content of selenium

Pending Publication Date: 2020-04-10
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Judging from the layout of relevant intellectual property rights, the current research on selenium-enriched lactic acid bacteria is only limited to a few strains of Lactobacillus; more importantly, the existing research is the use of lactic acid bacteria in synthetic media for chemical inorganic selenium reagents. (mainly sodium selenate), the relevant results have limited guiding significance for the food industry

Method used

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  • Selenium-rich lactic acid bacteria preparation and preparation method thereof
  • Selenium-rich lactic acid bacteria preparation and preparation method thereof

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Effect test

Embodiment 1

[0029] A preparation method for selenium-enriched lactic acid bacteria preparation, comprising the following steps:

[0030] (1) Activated strains: Inoculate the cryopreserved Lb.plantarum ST-III strains in MRS liquid medium, culture anaerobically at 37°C for 18-22 hours, subculture 2-3 times in a cycle, and obtain bacterial cultures Wherein the composition of MRS liquid medium is as follows: 10g peptone, 10g beef extract, 5g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1g Tween 80, 200mg magnesium sulfate, 54mg of manganese sulfate and 1000mL of distilled water, adjust the pH to 6.5, and sterilize at 121°C for 15 minutes;

[0031] (2) Preparation of Enshi selenium-enriched tea extract: Weigh 2g of Enshi selenium-enriched tea sample, add Soak in 100mL of pure water for 10min, take the supernatant, filter it through a 0.44μm sterile filter membrane, and get Enshi selenium-enriched tea extract;

[0032] (3) Con...

Embodiment 2

[0040] A preparation method for selenium-enriched lactic acid bacteria preparation, comprising the following steps:

[0041] (1) Activated strains: Inoculate the cryopreserved Lb.plantarum ST-III strains in MRS liquid medium, culture anaerobically at 37°C for 18-22 hours, subculture 2-3 times in a cycle, and obtain bacterial cultures Wherein the composition of MRS liquid medium is as follows: 10g peptone, 10g beef extract, 5g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1g Tween 80, 200mg magnesium sulfate, 54mg of manganese sulfate and 1000mL of distilled water, adjust the pH to 6.5, and sterilize at 121°C for 15 minutes;

[0042] (2) Preparation of ordinary green tea (Liu'an Guapian) extract: Weigh 2g of ordinary green tea (Liu'an Guapian) sample, add Soak in 100mL of pure water for 10min, take the supernatant, and filter through a 0.44μm sterile filter membrane to obtain ordinary green tea extract;

[0043...

Embodiment 3

[0049] A preparation method for selenium-enriched lactic acid bacteria preparation, comprising the following steps:

[0050] (1) Activated strains: freeze-preserved Lb.plantarum ATCC14917 strains (Whole-genome sequencing reveals the mechanisms for evolution of streptomycin resistance in Lactobacillus plantarum. Zhang F, Gao J, Wang B, Huo D, Wang Z, Zhang J, Shao Y.JDairy Sci.2018Apr; 101(4):2867-2874) was inoculated in MRS liquid medium, cultured anaerobically at 37°C for 18-22 hours, and subcultured 2-3 times in a cycle to obtain bacterial cultures; The composition of MRS liquid medium is as follows: 10g peptone, 10g beef extract, 5g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1g Tween 80, 200mg magnesium sulfate, 54mg sulfuric acid Manganese and 1000mL distilled water, adjust the pH to 6.5, and sterilize at 121°C for 15min;

[0051] (2) Preparation of Enshi selenium-enriched tea extract: Weigh 2g of Enshi s...

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Abstract

The invention discloses a preparation method for a selenium-rich lactic acid bacteria preparation. The preparation method comprises the following steps: (1) inoculating a cryopreserved Lb.plantarum ST-III strain into an MRS liquid culture medium, and carrying out anaerobic culture so as to obtain a thallus culture; (2) weighing 2 g of a green tea sample, adding purified water, carrying out soakingfor 10 min, taking a supernatant, and carrying out filtering with a sterile filtering membrane so as to obtain a green tea extract; (3) preparing an MRS-TE culture medium, then naturally cooling theMRS-TE culture medium to a room temperature, and adding the obtained green tea extract; (4) centrifuging the obtained thallus culture, and collecting thalli; (5) cleaning the collected thalli with normal saline for three times, re-suspending the thalli in the obtained MRS-TE culture medium, carrying out full and uniform shaking, and carrying out standing at 37 DEG C for fermentation; and (6) centrifuging the obtained fermented thallus, carrying out washing with sterile normal saline for three times, and carrying out centrifuging to remove the supernatant so as to obtain the selenium-rich lactic acid bacteria preparation. The preparation method provided by the invention has the advantage that organic selenium is efficiently enriched from the green tea by adopting a fermentation means.

Description

technical field [0001] The invention relates to the technical field of lactic acid bacteria preparations, in particular to a selenium-enriched lactic acid bacteria preparation and a preparation method thereof. Background technique [0002] Selenium is an essential trace mineral element for the human body and an important element for maintaining the normal growth and metabolism of life; it has been jointly confirmed by the Food and Agriculture Organization of the United Nations (Food and Agriculture Organization, FAO) and the World Health Organization (World Health Organization, WHO) as an essential element for the human body An important trace element, which is closely related to the maintenance of normal physiological functions of the body and the occurrence and development of various diseases, and is an essential component of many enzyme activity centers in mammals, such as glutathione peroxidase (glutathione peroxidase, GPx ), deiodinase (iodothyroninedeiodinases, ID), et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/25
CPCC12N1/20
Inventor 鄢明辉刘振民游春苹扶晓菲
Owner BRIGHT DAIRY & FOOD CO LTD
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