Radiation biological dosimeter, preparation method and application thereof

Abstract

The invention relates to a radiation biological dosimeter, a preparation method and application thereof, and belongs to the field of biotechnology. The biological dosimeter is a SoxR radiation biological dosimeter formed by adding the EGFP gene downstream of the SoxR promoter of Escherichia coli or a Cda radiation biological dosimeter formed by adding the EGFP gene downstream of the Cda promoter of Escherichia coli. The radiation biological dosimeter can monitor the size of physical radiation dose on line and in real time, and has a wide range of detection and application. The population effect of microorganisms is applied as a biological signal amplification system, and the sensitivity is greatly improved compared with existing related detection technologies. In the later period, the radiation biological dosimeter is placed in a microfluidic chip for cultivation, so that on-line real-time monitoring can be realized and commercial production can be realized. The radiation biological dosimeter is convenient to carry, is fast in detection, can provide the size of cytotoxic substances and radiation doses in the environment at any time, and provides a reference for detecting the size of damage doses.

Description

technical field [0001] The present invention relates to a kind of radiation biological dosimeter, preparation method and application, in particular, to a kind of biometric method using biotechnology to modify the gene sequence of microorganisms as the radiation dose, for the low linear energy transfer (LinearEenergy Transfer, LET) physical Radiation and ultraviolet radiation are monitored, the detection time is short, the results are intuitive and visible, and there is a certain dose-effect relationship between the response intensity and the radiation dose. The fluorescence intensity value reflects the cytotoxicity of the radiation in the environment, and it is harmful to chemical cells. The reagent has response and detection broad-spectrum; it belongs to the field of biotechnology. Background technique [0002] Water pollution, noise pollution, air pollution and radiation are known as the four major environmental pollution. According to the biological effects of radiation,...

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Problems solved by technology

[0013] In view of the above-mentioned existing technologies only for the detection of chemical toxic reagents, less attention is paid to radiation factors, and the detection time of the Ames method in the previous detection methods is long, and the chemical sensor detection method has many interference signals, and there are high detection backgrounds and high sensitivity. Low defect, one of the purposes of the present invention is to provide a radiation biological dosimeter, which uses genetically engineered bacteria as a microbial sensor, and directly reflects the radiation dose through the fluorescent signal, and effectively uses the group effect to amplify the signal, innovatively established A radiation biological dosimeter. Compared with other chemical dosimeters, the radiation biological dosimeter has many advantages: non-toxic and harmless, convenient for online detection and preparation, low cost, and the interaction between organisms can be used. The group effect among microorganisms can realize signal amplification, facilitate detection, and improve the detection sensitivity of radiation biological dosimeters

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