An endophytic fungus z1 that increases the biomass of casuarina under high-salt environment

A technology of endophytic fungi and casuarina, which is applied in the field of microorganisms, can solve the problems of less research on endophytic fungi in woody plants, and achieve the effect of promoting plant biomass and increasing growth

Active Publication Date: 2021-08-31
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A series of previous studies have shown that the symbiont between plant endophytic fungi and the host is beneficial for the host to cope with the adverse environment and promote the growth and development of the plant. Less research on endophytic fungi

Method used

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  • An endophytic fungus z1 that increases the biomass of casuarina under high-salt environment
  • An endophytic fungus z1 that increases the biomass of casuarina under high-salt environment
  • An endophytic fungus z1 that increases the biomass of casuarina under high-salt environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Isolation of casuarina ephedra fungus

[0016] 1. Main equipment

[0017] AX224ZH electronic balance (OHAUS), LS-75HD high-pressure steam sterilizer (Jinan Laibao Medical Equipment Co., Ltd.), JB-CJ-1500FX ultra-clean workbench (Suzhou Jiabao Purification Engineering Equipment Co., Ltd.), DNP-9162 constant temperature culture box (Shanghai Jinghong), ZHWY2111B constant temperature shaking incubator (Shanghai Zhicheng), Beckman ultracentrifuge (USA), etc. Main instruments for strain identification: FQD-48APCR amplification instrument (BIOER), EPS-100 nucleic acid electrophoresis instrument (Shanghai Tianneng Technology), SeqStudio DNA sequencer (USA), mortar, ice pack, foam box, pipette (Thermo), 1.5ml and 2ml EP tubes (Axygen).

[0018] 2. Main Reagents and Media

[0019] Reagents: 70% alcohol, sodium hypochlorite, potato dextrose agar (PDA) medium, potato dextrose (PDB) medium, NaCl, Taq enzyme and PCR related reagents, primers (ITS1 / ITS4), OMEGA fungal ge...

Embodiment 2

[0037] Preparation of bacteria solution: inoculate the activated halotolerant Vitis vinifera endophyte Z1 strain in 60 mL potato dextrose liquid medium, and culture it on a constant temperature shaker for 72 hours (28 °C, 160 r min -1 ). Calculate the number of spores by hemocytometer, and dilute the cultivated bacterial solution to 5.5×10 by ten-fold dilution method with sterile water. 6 pcs L -1 .

[0038]This experiment adopts the soil cultivation pot experiment. The casuarinas selected for the experiment are annual seedlings with an average seedling height of 20cm and an average ground diameter of 3.0mm. They were provided by the Chihu State-owned Forest Farm in Hui'an, Fujian Province. On July 3, 2017, casuarinas with consistent growth were selected and planted in plastic pots with a diameter of 15 cm and a height of 10 cm. The soil required for the experiment was mixed and weighed, and an equal amount (4kg) of yellow core soil was placed in each pot. The nutrient cont...

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Abstract

The invention discloses an endophytic fungus Z1 capable of increasing the biomass of casuarina under a high-salt environment, belonging to the field of microorganisms. The taxonomic name of the endophytic fungus Z1 is: Botrytis ( Botryosphaeria sp. ), was deposited in the General Microorganism Center of China Committee for Culture Collection of Microorganisms on November 20, 2019; the culture preservation number is: CGMCC NO.18817. The bacterial solution of endophytic fungus Z1 can be used to plant casuarina seedlings in high-salt environment by watering rhizosphere soil or direct inoculation of seedlings, which can increase the biomass of casuarina. By establishing the casuarina-endophytic fungal symbiotic system, the growth of casuarina under high-salt stress can be improved, and the data basis and reference can be provided for enriching the species information of endophytic fungi in woody plants and for the production of biological fertilizers in casuarina industrial forests in accordance with.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an endophytic fungus Z1 which can increase the biomass of casuarina under high-salt environment. Background technique [0002] Salt stress seriously affects the growth and development of crops and forest plants, and is the main abiotic stress worldwide. Excess salt ions, such as Na + 、K + , Cl - After being ingested by plants, the ion concentration in plant cells will increase, and various biological enzymes in plant cells can only be active in a narrow range of ion concentrations, such as Na + ions and Cl - Ion requirements below 50mM for K + For ions, it is 0.1-0.2M. Therefore, changes in ion concentration affect the activity of biological enzymes, thereby affecting the growth of plants. Salt stress can also damage the plant cell membrane function by affecting a series of changes such as the composition, permeability, and transport of the plasma membra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G17/00A01P21/00C12R1/645
CPCA01G17/005C12N1/145C12R2001/645
Inventor 李键龙凤洪滔林勇明谢安强
Owner FUJIAN AGRI & FORESTRY UNIV
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