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Granular threonine extraction and preparation process

A preparation process, technology of threonine, applied in the field of separation, extraction and preparation of granular threonine, can solve the problems of easy moisture absorption and agglomeration, reduce the use value of mother liquor, and unfavorable addition, so as to improve quality and added value, Ease of storage and transportation, and improve fluidity

Inactive Publication Date: 2020-04-17
齐齐哈尔龙江阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this process, the concentrated solution of the L-threonine fermentation broth intercepted by the ceramic membrane contains a large amount of L-threonine. If this part of the feed solution is directly subjected to flocculation and sedimentation treatment, the utilization value of the mother liquor will be reduced, and it will be discharged to The environment will cause environmental pollution and waste of resources
In addition, due to the small particle size of powdered threonine, it is easy to absorb moisture and agglomerate, which is not conducive to transportation and storage. In addition, during the process of feed addition and use, the fluidity is poor and the dust is large, which is not conducive to addition, which brings a negative impact on the development of threonine industry. here comes a certain limit

Method used

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  • Granular threonine extraction and preparation process

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Including the following steps:

[0025] Step 1) Fermentation: Escherichia coli engineering bacteria seed solution (the concentration of the seed solution is 1×10 8 cfu / mL) according to 10% of the inoculum amount into a fermenter containing fermentation medium for fermentation, the temperature is 30°C, the tank pressure is 0.04MPa, the ventilation rate is 0.5vvm, the rotation speed is 100rpm, and the fermentation time is 36h, then Inject Chlamydomonas reinhardtii according to 10% inoculum (the concentration of Chlamydomonas reinhardtii is 1×10 5 cfu / mL), continue to ferment for 48 hours, stop the fermentation, and collect the fermented liquid;

[0026] The components of the fermentation medium are: glucose 20g / L, glycerol 20g / L, corn steep liquor 20g / L, ammonium sulfate 2g / L, potassium dihydrogen phosphate 0.2g / L, dipotassium hydrogen phosphate 0.2g / L, Magnesium sulfate heptahydrate 0.1g / L, ferrous sulfate heptahydrate 0.01g / L, manganese sulfate monohydrate 0.01g / L, pH...

Embodiment 2

[0036] Including the following steps:

[0037] Step 1) Fermentation: Escherichia coli engineering bacteria seed solution (the concentration of the seed solution is 1×10 8cfu / mL) according to the inoculation amount of 6%, was inserted into the fermenter containing the fermentation medium for fermentation, the temperature was 32°C, the tank pressure was 0.04MPa, the ventilation rate was 0.4vvm, the rotation speed was 100rpm, and the fermentation time was 48h. Then insert Chlamydomonas reinhardtii (the concentration of Chlamydomonas reinhardtii is 1×10 5 cfu / mL), continue to ferment for 48 hours, stop the fermentation, and collect the fermented liquid;

[0038] The components of the fermentation medium are: glucose 30g / L, glycerol 30g / L, corn steep liquor 30g / L, ammonium sulfate 3g / L, potassium dihydrogen phosphate 0.3g / L, dipotassium hydrogen phosphate 0.3g / L, Magnesium sulfate heptahydrate 0.2g / L, ferrous sulfate heptahydrate 0.02g / L, manganese sulfate monohydrate 0.02g / L, pH...

Embodiment 3

[0048] Including the following steps:

[0049] Step 1) Fermentation: Escherichia coli engineering bacteria seed solution (the concentration of the seed solution is 1×10 8 cfu / mL) according to 10% of the inoculum amount into a fermenter containing fermentation medium for fermentation, the temperature is 30°C, the tank pressure is 0.04MPa, the ventilation rate is 0.5vvm, the rotation speed is 100rpm, and the fermentation time is 36h, then Inject Chlamydomonas reinhardtii according to 10% inoculum (the concentration of Chlamydomonas reinhardtii is 1×10 5 cfu / mL), continue to ferment for 48 hours, stop the fermentation, and collect the fermented liquid;

[0050] The components of the fermentation medium are: glucose 20g / L, glycerol 20g / L, corn steep liquor 20g / L, ammonium sulfate 2g / L, potassium dihydrogen phosphate 0.2g / L, dipotassium hydrogen phosphate 0.2g / L, Magnesium sulfate heptahydrate 0.1g / L, ferrous sulfate heptahydrate 0.01g / L, manganese sulfate monohydrate 0.01g / L, pH...

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Abstract

The invention belongs to the technical field of amino acid production, and discloses a granular threonine extraction and preparation process. The granular threonine extraction and preparation processcomprises the following steps: step 1) fermentation; step 2) fermentation liquor separation and extraction; step 3) concentration and crystallization; step 4) crystal separation; step 5) drying; step6) primary mother liquor crystallization and separation; step 7) chromatographic separation; step 8) crystallization and separation; and step 9) granulation. The extraction process comprises that in aseparation and extraction stage after threonine fermentation, threonine fermented mash is treated by using a ceramic membrane and a horizontal screw centrifuge in a combined manner, and granular threonine is obtained through a dry rolling granulation process. The process can improve the yield of granular threonine, is simple and feasible, effectively reduces the environment-friendly sewage treatment pressure and production loss, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of amino acid production, and in particular relates to a separation, extraction and preparation process of granular threonine. Background technique [0002] L-threonine (Threonine, abbreviated as Thr) is a white crystal or crystalline powder, a polar amino acid, odorless, slightly sweet taste. It melts and decomposes at 253°C. Soluble in water at high temperature, the solubility at 25°C is 20.5g / 100ml, insoluble in ethanol, ether and chloroform. The specific optical rotation is 27.6°-29°, and the isoelectric point pI is 5.64. [0003] L-threonine is an essential amino acid that constitutes human, animal and plant proteins. It is mainly used in the fields of medicine, chemical reagents, food fortifiers and feed additives. Especially in the field of feed additives, the amount is growing rapidly. It is often added to the feed of young piglets and poultry. It is the second limiting amino acid in pig feed and the third...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C227/40C07C229/22
CPCC07B2200/07C07C227/40C07C229/22
Inventor 韩杨关健董力青高雅坤冯珍泉王建文卢树军
Owner 齐齐哈尔龙江阜丰生物科技有限公司
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