Material supplementing control method for chemoautotrophic microorganism culture process

Abstract

The invention relates to a material supplementing control method for chemoautotrophic microorganism culture process, which comprises the following steps: adding a microorganism culture medium into anaeration reactor, and adjusting the aeration rate without inoculation until the DO concentration is not increased any more, thereby determining the DOmax of the culture system and the corresponding aeration rate; after microorganism inoculation, until DO tends to be lowest and is stabilized for 1-2 h, controlling DO concentration at the moment to be not lower than 5% DOmax; in the microorganism culture process, when DO rises to 30%-70% DOmax, turning on a material supplementing system, and stopping material supplementing when DO falls by 10%-50%; and repeating the steps until the culture process is finished. The microorganisms obtained through the method are high in activity, rapid growth and propagation can be achieved, and the problem that a bacterium source needed by industrial application grows slowly is solved.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a feeding control method in the culture process of chemoautotrophic microorganisms. The method can realize the rapid growth and reproduction of chemoautotrophic microorganisms with high activity. Background technique [0002] Autotrophic microorganisms include photoautotrophs and chemoautotrophs. Among them, many bacteria in the sewage treatment process belong to chemoautotrophic microorganisms, which mainly obtain energy from the oxidation of inorganic substances, and use the energy released by oxidation to produce ATP to assimilate carbon dioxide to produce nutrients needed by synthetic cells. Therefore, such microorganisms grow slowly, have a long generation time, and have a slow value-added speed, and are not easy to be quickly cultivated, and it is difficult to apply them in large scale in industry. Therefore, for autotrophic microorganisms, in additi...

AI Technical Summary

Problems solved by technology

This method is to add the trace element mixture at a specific time, and control the amount and way of adding glucose in combination with the change of sugar content. There are many indicators to be monitored and the operation is complicated.

[0006] The existing technology is based on the aerobic and facultative oxygen conditions of the system, controls the dissolved oxygen within a certain range, and provides the required dissolved oxygen concentration for the growth of microorganisms. The feeding method involved is mainly based on the utilization of the substrate Supplement the appropriate amount of substrates to provide the required substrate concentration for the growth of microorganisms. The adaptability between the control parameters is not good, and the microbial activity needs to be improved.