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Multifunctional pediococcus acidilactici CCFM1105, fermented food produced from multifunctional pediococcus acidilactici CCFM1105 and application of multifunctional pediococcus acidilactici CCFM1105

A CCFM1105, Pediococcus lactis technology, applied in the field of microorganisms, can solve the problems of high price and difficult to obtain, and achieve the effects of reducing content, improving liver enlargement, and removing reducing ability

Active Publication Date: 2020-05-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these natural substances are expensive and difficult to obtain. In addition, due to the tolerance of the human body, it is not yet known whether a large amount of intake will cause potential harm to the human body.

Method used

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  • Multifunctional pediococcus acidilactici CCFM1105, fermented food produced from multifunctional pediococcus acidilactici CCFM1105 and application of multifunctional pediococcus acidilactici CCFM1105
  • Multifunctional pediococcus acidilactici CCFM1105, fermented food produced from multifunctional pediococcus acidilactici CCFM1105 and application of multifunctional pediococcus acidilactici CCFM1105
  • Multifunctional pediococcus acidilactici CCFM1105, fermented food produced from multifunctional pediococcus acidilactici CCFM1105 and application of multifunctional pediococcus acidilactici CCFM1105

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Pediococcus lactis CCFM1105 has good PFOS adsorption capacity

[0066] Bacterial adsorption purification and activation culture of Pediococcus lactis CCFM1105, inoculated into MRS liquid medium at 1% (v / v) inoculum, and cultivated at 37°C for 12 hours. Then the cells were collected by centrifugation at 8000r / min for 5min, the precipitate was cleaned up with saline and then centrifuged at 8000r / min for 5min to remove the precipitation to obtain live cells, namely wet cells. Suspend the wet bacteria weight in a 20mg / L PFOS solution and make the final bacterial cell concentration reach 1g dry bacteria / L (suspend the wet bacteria weight in ultrapure water without PFOS as a blank control). Use 0.1M NaOH or HCl solution to quickly adjust the pH of the PFOS solution containing bacteria to 3.0, add a small amount of NaOH or HCl (less than 0.5ml), the ionic strength of the effect on the PFOS adsorption can be ignored. Subsequently, a 250ml Erlenmeyer flask containing 10...

Embodiment 2

[0071] Example 2: Pediococcus lactis CCFM1105 has a good ability to scavenge diphenyltrinitrophenylhydrazine free radicals (DPPH), hydroxyl free radicals and reducing ability in vitro;

[0072] After thoroughly mixing 1 mL of Pediococcus lactis CCFM1105 intact cell suspension with 1 mL of freshly prepared DPPH absolute ethanol solution (0.2 mmol / L), react for 30 min at 37°C in the dark. Mix DPPH and PBS (pH 7.2) as a control sample, and culture under the same conditions. After centrifugation at 7000×g for 10 minutes, the absorbance was measured at 517nm, and the ability of lactic acid bacteria to scavenge DPPH free radicals was calculated according to the following formula:

[0073] DPPH radical scavenging rate (%)=[1-A517 (sample) / A517 (control)]×100%.

[0074] Mix 1 mL 1,10-phenanthroline 1 mL PBS (pH 7.2), 1 mL Pediococcus lactis CCFM1105 intact cell suspension or 1 mL FeSO4 (referred to as "mixture 1"). Add 1mLH to "Mixture 1" 2 O 2 Measure the absorbance at 536nm in a water ba...

Embodiment 3

[0077] Example 3: Pediococcus lactis CCFM1105 can significantly improve liver enlargement in PFOS-exposed mice 24 6-week-old male C57BL / 6J mice. After one week of adaptation to the environment, they were randomly divided into four groups according to their body weight: blank control group, model group, Quercetin intervention group, Pediococcus lactis CCFM1105 intervention group, each group contains 6 mice, animal grouping and treatment methods are shown in Table 2.

[0078] Table 2 Animal experiment grouping and treatment methods

[0079]

[0080] The mice in Example 2 were weighed on the 31st day and then euthanized, blood was taken from the orbit, and the liver was taken out and weighed to calculate the organ coefficient. The organ coefficient of the mouse liver was calculated according to the following formula:

[0081] Liver organ coefficient = liver wet weight (g) / mouse weight before euthanasia (100g)

[0082] The experimental results are as image 3 As shown, the results indic...

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Abstract

The invention discloses multifunctional pediococcus acidilactici CCFM1105, fermented food produced from the multifunctional pediococcus acidilactici CCFM1105 and application of the multifunctional pediococcus acidilactici CCFM1105. The pediococcus acidilactici CCFM1105 has excellent capability of adsorbing PFOS in vitro so that the concentration of the PFOS can be decreased, has good capability ofscavenging 1,1-diphenyl-2-picrylhydrazyl free radicals and scavenging hydroxyl free radicals and good reducing capability, improves hepatomegaly, caused by exposure to PFOS, of mice, raises the levels of CAT, SOD and antioxidant capability, improves intestinal flora disturbance, reduces abundance of an S24-7 family, lactobacillus, bifidobacterium and parabacteroides in intestinal tracts of mice,and reduces the incidences of diseases of liver diseases, hypertension, diabetes, obesity and the like.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to multifunctional Pediococcus lactis CCFM1105, its fermented food and applications. Background technique [0002] Perfluorinated compounds have been widely used in various industries due to their hydrophobic and oleophobic properties, as well as good thermal, chemical and biological stability. Used in ready-to-wear (such as waterproof, anti-fouling outdoor clothing) and home textiles (such as carpets, furniture fabrics, etc.), take-out food containers, personal care products (such as dental floss) and fire fighting foam, etc. Among them, PFOS, as one of the final conversion products of a variety of fluorine compounds, can directly cause harm to the human body through environmental exposure, or enter the body of animals and plants to bioaccumulate in the human body through the food chain, so it has been increasingly attracted by researchers. The more attention. Althou...

Claims

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Application Information

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IPC IPC(8): A23C9/12A23C13/16A23C19/032A23L11/00A23L19/20A23L29/00A61K35/744A61P1/16A61P3/04A61P3/10A61P9/12C12N1/20C12R1/01A23L11/50
CPCA23L19/20A23L29/065A23C9/1203A23C13/16A23C19/0323A61K35/744A61P1/16A61P3/04A61P3/10A61P9/12A23L11/50C12R2001/01C12N1/205A23V2400/413
Inventor 王刚陈卫孙姗姗乌翛冰赵建新张灏
Owner JIANGNAN UNIV
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