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Plant-derived exosome, and preparation method and application thereof

A plant-derived exosome technology, applied in the field of bioengineering, achieves good application prospects and research value, easy to control, and structurally complete effects

Active Publication Date: 2020-05-12
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still a lack of related applications and research on the extraction of plant exosomes and tissue engineering therapy.

Method used

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  • Plant-derived exosome, and preparation method and application thereof
  • Plant-derived exosome, and preparation method and application thereof
  • Plant-derived exosome, and preparation method and application thereof

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Embodiment Construction

[0032] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The operating methods not indicated in the following examples are generally in accordance with conventional conditions, or in accordance with the conditions suggested by the manufacturer.

[0033] 1. Preparation method of plant-derived exosomes:

[0034] 2-(N-morpholine) ethanesulfonic acid 4.26g, NaCl 5.85g, CaCl 2 ·H 2 O 0.27g was dissolved in ultrapure water, adjusted to pH 6.0 with 5M NaOH solution, and prepared 2-(N-morpholine)ethanesulfonic acid buffer solution;

[0035] Soak dried Sophora japonica in the above buffer solution for 12-18 hours, vacuumize for 10 seconds after soaking for the preset time, with an interval of 30 seconds between each time, a total of 3 times;

[0036]...

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Abstract

The invention discloses a plant-derived exosome and a preparation method thereof, and application of the plant-derived exosome in preparation of drugs or scaffolds for animal tissue regeneration therapy. The preparation method comprises the following steps: (1) taking any part of a natural plant, performing a 2-(N-morpholino)ethanesulfonic acid buffer soaking and permeation treatment, then discarding a supernatant, collecting a treated wet sample, and performing refrigerated centrifugation to extract and obtain an apoplast solution, wherein the soaking and permeation treatment is specificallyas follows: vacuumizing is performed 2 to 5 times after 6 to 24 hours of soaking, a time for each vacuumizing process is independently 5 to 15 seconds, and an interval between every two adjacent vacuumizing processes is independently 10 seconds to 1 minute; and (2) performing ultrahigh-speed centrifugation on the apoplast solution to obtain the plant-derived exosome, wherein conditions of the ultrahigh-speed centrifugation are as follows: a centrifugal force is not less than 100000g, a centrifugation time is 1 to 7 hours, and a temperature is 0 to 4 DEG C.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a plant-derived exosome and its preparation method and application. Background technique [0002] Tissue damage, including nerve tissue damage, bone damage, skin damage, etc., is a class of diseases that widely threaten human health. Inhibitory injury microenvironment is one of the main factors hindering tissue regeneration. After acute trauma, accompanied by ischemia and edema, severe oxidative stress occurs in the injured area, and excessive reactive oxygen species (ROS) are produced. Can further cause secondary damage to the tissue. Therefore, the regulation of the damaged local peroxidative microenvironment is an important approach for tissue regeneration therapy. [0003] Several plants have been proven to have antioxidant properties, and natural antioxidant drugs derived from plants, such as rutin, anthocyanins, curcumin, etc., have been used in antioxidant researc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A61K36/489A61P25/00A61P39/06A61L27/36A61L27/50
CPCC12N5/04A61K36/489A61P25/00A61P39/06A61L27/3637A61L27/3675A61L27/3687A61L27/3691A61L27/50C12N2509/00A61L2300/30A61L2400/06A61L2430/32A61K2236/333A61K2236/53
Inventor 高建青李黎明陈家宸李平
Owner ZHEJIANG UNIV
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