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Crocodile peptide chelated calcium as well as preparation method and application thereof

A chelated calcium, crocodile technology, applied in the application, food science, function of food ingredients, etc., can solve the problems of high blood sugar, low apparent absorption rate, toxic and side effects in diabetic patients, and achieve high bioavailability, surface The effect of high absorption rate, easy absorption and retention

Inactive Publication Date: 2020-05-19
福建鼍龙实业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many calcium supplement preparations at home and abroad, but inorganic calcium salts are dominant. Inorganic calcium salts are cheap but have a low apparent absorption rate and tend to stay in the kidneys, leading to diseases such as kidney stones. Long-term use of calcium carbonate may cause dry stools and loss of appetite , stomach pain and other side effects
In recent years, domestic and foreign studies on calcium supplements have focused on organic calcium, but organic calcium also has different toxic and side effects, such as calcium lactate is prone to fatigue when taken for a long time; calcium gluconate can cause blood sugar to rise in diabetic patients; calcium acetate can cause diarrhea etc.

Method used

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  • Crocodile peptide chelated calcium as well as preparation method and application thereof
  • Crocodile peptide chelated calcium as well as preparation method and application thereof
  • Crocodile peptide chelated calcium as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] A kind of preparation method of crocodile peptide chelated calcium, concrete steps are as follows:

[0086] Clean the crocodile meat, remove the fat with a knife and cut it into small pieces about 1 cm square. Take 500 grams of crocodile meat, add 1-2 liters of water, boil for 30 minutes, take it out after boiling, and dry it in an oven at 60°C for 10 minutes. Weigh 50g, add water with 8 times the mass of crocodile meat, stir with a mixer to form a homogenate, add papain with 3% mass of crocodile meat, and bathe in a water bath at 60°C for 5 hours to obtain an enzymatic hydrolysis solution. The enzymolysis solution was boiled in a water bath for 10 minutes to inactivate the enzyme. After cooling to room temperature, it was centrifuged at 12000 rpm for 20 minutes, then suction filtered, and finally the filtrate was sent to vacuum freeze-drying to obtain crocodile peptide powder.

[0087] Dissolve the crocodile peptide powder in water to make a solution with a concentrati...

Embodiment 2

[0089] A kind of mensuration of crocodile peptide chelated calcium chelation rate, concrete steps are as follows:

[0090] Weigh 0.2g of crocodile peptide chelated calcium sample into a microwave digestion tube, add 8ml of concentrated nitric acid, leave it at room temperature for 30min, and then digest it with a microwave digestion apparatus. After digestion, rinse with acid and dilute to 100ml with double distilled water. After taking 1ml of the solution and diluting it to an appropriate multiple, measure the calcium content of the solution with an inductively coupled plasma mass spectrometer (ICP-MS). Calculated as follows:

[0091]

[0092] In the above formula: c is that ICP-MS records the calcium content of solution, μ g / ml; n is the dilution factor; v is the volume after constant volume, 100ml; m 1 The chelated calcium quality that embodiment 1 prepares; m 2 is the mass of the sample taken, 0.2g; m3 is the CaCl added during preparation 2 mass, g; 40 is the relati...

Embodiment 3

[0094] The influence of different peptide calcium mass ratios on the chelation rate, the specific steps are as follows:

[0095] Clean the crocodile meat, remove the fat with a knife and cut it into small pieces about 1 cm square. Take 500 grams of crocodile meat, add 1-2 liters of water, boil for 30 minutes, take it out after boiling, and dry it in an oven at 60°C for 10 minutes. Weigh 50g, add water with 8 times the mass of crocodile meat, stir with a mixer to form a homogenate, add papain with 3% mass of crocodile meat, and bathe in a water bath at 60°C for 5 hours to obtain an enzymatic hydrolysis solution. The enzymolysis solution was boiled in a water bath for 10 minutes to inactivate the enzyme, cooled to room temperature, centrifuged at 12,000 rpm for 20 minutes, then suction filtered, and finally the filtrate was sent to vacuum freeze-drying to obtain crocodile peptide powder.

[0096] Take six 500ml blue cap bottles, add 1g, 2g, 3g, 4g, 5g, 6g of crocodile peptide po...

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Abstract

The invention discloses crocodile peptide chelated calcium. The crocodile peptide chelated calcium comprises 70%-85% by mass of the alligator peptide and 5%-10% by mass of calcium. The crocodile peptide chelated calcium is more easily absorbed and retained, while inorganic calcium is discharged out of the body.

Description

technical field [0001] This application relates to the field of biological products, especially but not limited to an alligator peptide chelated calcium and its preparation method and application. Background technique [0002] Calcium is an important mineral for living organisms to maintain health. In the human body, the calcium content accounts for about 1%-2% of the body mass (about 1200 grams for adults). About 99% of Ca is in the form of phosphate in bones and teeth, providing hardness and structure. Calcium has many biological functions such as intracellular metabolism, blood coagulation, nerve conduction, muscle contraction, cardiac function, bone growth and bone support structure. In China, the recommended dietary allowance of calcium for adults is about 800 mg a day, and for teenagers and the elderly, it is increased to 1200 mg a day. Calcium intake is critical for maintaining bone density and maximizing protection from negative calcium balance, especially in the ...

Claims

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Application Information

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IPC IPC(8): A23L33/165C12P21/06
CPCA23L33/165C12P21/06A23V2002/00A23V2200/30A23V2250/1578
Inventor 董欣黄志杰丁玉梅熊奕琳熊佑熊陈清西
Owner 福建鼍龙实业有限责任公司
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